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1. Kadiene EU, Ouddane B, Gong HY, Kim MS, Lee JS, Pan YJ, Hwang JS*, Souissi S*., "Differential gene expression profile of male and female copepods in response to cadmium exposure," Ecotoxicology and Environmental Safety 204 (2020) 111048., https://doi.org/10.1016/j.ecoenv.2020.111048., Aug, 2020.
https://authors.elsevier.com/c/1bW8MXad0VsI9
2. Chiang KY, Lin WC, Tsai TY, Lin CW, Huang SJ, Huang CY, Wu SH, Ken CF, Gong HY*, Chen JY*, Wu JL*., "Dual expression of transgenic delta-5 and delta-6 desaturase in tilapia alters gut microbiota and enhances resistance to Vibrio vulnificus infection," PLoS ONE 15(7): e0236601. https://doi.org/ 10.1371/journal.pone.0236601, Jul, 2020.
Attachments:
1. journal.pone.0236601.pdf
3. Hsu TH, Huang CW, Lee HT, Kuo YH, Liu KM, Lin CH, Gong HY*., "Population genetic analysis for stock enhancement of silver sea bream (Rhabdosargus sarba) in Taiwan.," Fishes (2020) 5, 19. doi:10.3390/fishes5020019, Jun, 2020.
Attachments:
1. fishes-05-00019-v2.pdf
4. Wu SH, Chou HY, Liu PC, Wu JL, and Gong HY*., "Granulin peptide GRN-41 of Mozambique tilapia is a novel antimicrobial peptide against Vibrio species.," Biochemical and Biophysical Research Communications (2019) 515: 706-711., https://doi.org/10.1016/j.bbrc.2019.06.022, Jun, 2019.
In our previous study, the novel GRN-41 peptide generated from alternative splicing of the Mozambique tilapia PGRN1 gene was identified to be a potent peptide that protected against V. vulnificus in the transgenic zebrafish model by modulating innate immune-related genes. In this study, the anti-bacterial activities of synthetic Mozambique tilapia GRN-41 peptide (OmGRN-41) against various bacterial pathogens were investigated. The results showed that OmGRN-41 had bactericidal activity against Vibrio species, including V. vulnificus, V. alginolyticus, and V. harveyi, but exhibited bacteriostatic activity against V. parahaemolyticus. OmGRN-41 maintained bactericidal activity (64 μM) against V. vulnificus at pH 2 to pH 10 or after heat treatment for 1 h at high temperatures between 40 °C and 100 °C. TEM observations revealed that the outer membrane of V. vulnificus was disrupted by OmGRN-41, leading to morphological rupture and loss of cytoplasmic contents. Additionally, little hemolytic activity against tilapia and sheep erythrocytes was detected after treatment with 128 μM OmGRN-41. OmGRN-41 can effectively enhance the survival of Nile tilapia infected by V. vulnificus. Our results suggest that the OmGRN-41 is a novel antimicrobial peptide possessing bactericidal activity, especially against Vibrio species. These results indicate that OmGRN-41 can be applied in human Vibriosis treatment and has the potential to defend against Vibrio spp. infection in critical aquaculture organisms.
5. Chu PY, Kuan JH, TunCC, Hsu TH, Gong HY, Lin CH, Huang CW*., "Establishment of a Database of Microsatellite Markers for the Yellowfin Seabream (Acanthopagrus latus) in the Coastal Sea of Taiwan," J. Fish. Soc. Taiwan, 2018, 45(2): 59-64., Mar, 2018.
6. Hsu TH, Lee HT, Chen JY, Gong HY, Huang CW*., "Development of microsatellite multiplex PCR assays for the black seabream (Acanthopagrus schlegelii).," J. Fish. Soc. Taiwan, 2018, 45(2): 65-75., Mar, 2018.
7. Wu SH, Lin HJ, Lin WF, Wu JL and Gong HY*, "A potent tilapia secreted granulin peptide enhances the survival of transgenic zebrafish infected by Vibrio vulnificus via modulation of innate immunity," Fish & Shellfish Immunology 75 (2018): 74-90. (https://doi.org/10.1016/j.fsi.2018.01.044), (IF: 3.148), Marine & Freshwater Biology (Ranking: 7/105=6.7%), Jan, 2018.
Progranulin (PGRN) is a multi-functional growth factor that mediates cell proliferation, survival, migration, tumorigenesis, wound healing, development and anti-inflammation activity. A novel alternatively spliced transcript from short-form PGRN1 gene encoding a novel, secreted GRN peptide composed of 20-a.a. signal peptide and 41-a.a. GRN named GRN-41 was identified to be abundantly expressed in immune-related organs including spleen, head kidney, gill and intestine of Mozambique tilapia. The expression of GRN-41 and PGRN1 were further induced in the spleen of tilapia challenged with Vibrio vulnificus at 3 hours post infection (hpi) and 6 hpi, respectively. In this study, we established three transgenic zebrafish lines expressing the secreted GRN-41, GRN-A and PGRN1 of Mozambique tilapia specifically in muscle. The relative percent of survival (RPS) was enhanced in adult transgenic zebrafish expressing tilapia GRN-41 (68%), GRN-A (32%) and PGRN1 (36%) compared with control transgenic zebrafish expressing AcGFP after challenge with V. vulnificus. It indicates tilapia GRN-41 is a potent peptide against V. vulnificus infection. The secreted tilapia GRN-41 can induce the expression of innate immune response-related genes, such as TNFa, TNFb, IL-8, IL-1β, IL-6, IL-26, IL-21, IL-10, complement C3, lysozyme (Lyz) and the hepatic antimicrobial peptide hepcidin (HAMP), in adult transgenic zebrafish without V. vulnificus infection. The tilapia GRN-41 peptide can enhance the innate immune response by further elevating TNFb, IL-1β, IL-8, IL-6, and HAMP expression in early responsive time to the V. vulnificus challenge in transgenic zebrafish. Our results suggest that the novel GRN-41 peptide generated from alternative splicing of the tilapia PGRN1 gene is a potent peptide that defends against V. vulnificus in the transgenic zebrafish model by modulation of innate immunity.

Keywords: Tilapia; Progranulin; Granulin; Innate immunity; Vibrio; Transgenic zebrafish; Alternative splicing
8. Huang SJ, Cheng CL, Chen JR, Gong HY, Liu W, Wu JL*., "Inducible liver-specific overexpression of gankyrin in zebrafish results in spontaneous intrahepatic cholangiocarcinoma and hepatocellular carcinoma formation.," Biochem Biophys Res Commun. 490: 1052-1058., Jun, 2017.
Liver cancer is the second leading cause of death worldwide. As such, establishing animal models of the disease is important for both basic and translational studies that move toward developing new therapies. Gankyrin is a critical oncoprotein in the genetic control of liver pathology. In order to evaluate the oncogenic role of gankyrin without cancer cell inoculation and drug treatment, we overexpressed gankyrin under the control of the fabp10a promoter. A Tet-Off system was used to drive expression in hepatocytes. At seven to twelve months of age, gankyrin transgenic fish spontaneously incurred persistent hepatocyte damage, steatosis, cholestasis, cholangitis, fibrosis and hepatic tumors. The tumors were both hepatocellular carcinoma (HCC) and intrahepatic cholangiocarcinoma (ICC). ICC is the second most frequent primary liver cancer in human patients and the first to develop in this tumor model. We further investigated the role of complement C3, a central molecule of the complement system, and found the expression levels of both in mRNA and protein are decreased during tumorigenesis. Together, these findings suggest that gankyrin can promote malignant transformation of liver cells in the context of persistent liver injury. This transformation may be related to compensatory proliferation and the inflammatory microenvironment. The observed decrease in complement C3 may allow transforming cells to escape coordinated induction of the immune response. Herein, we demonstrate an excellent zebrafish model for liver cancers that will be useful for studying the molecular mechanisms of tumorgenesis.
9. Gong HY*, Wu SH, Chen CY, Huang CW, Lu JK, Chou HY, "Complete Genome Sequence of Streptococcus iniae 89353, a Virulent Strain Isolated from Diseased Tilapia in Taiwan," Genome Announcements 5: e01524-16., Jan, 2017.
10. Pan CY, Liu YH, Gong HY, Chen JY*., "Transcriptome analysis of the effect of polyunsaturated fatty acids against Vibrio vulnificus infection in Oreochromis niloticus.," Fish & Shellfish Immunology 62:153-163., Jan, 2017.
11. Chen YY, Chen JC*, Kuo YH, Lin YC, Chang YH, Gong HY, Huang CL., "Lipopolysaccharide and β-1,3-glucan-binding protein (LGBP) bind to seaweed polysaccharides and activate the prophenoloxidase system in white shrimp Litopenaeus vannamei," Developmental and Comparative Immunology 55:144-151., Feb, 2016.
Lipopolysaccharide and β-1,3-glucan-binding protein (LGBP), important pattern recognition proteins (PRPs), recognize lipopolysaccharide (LPS) and β-1,3-glucan (βG), known as pathogen-associated molecular patterns (PAMPs), and subsequently trigger innate immunity. Several seaweed polysaccharides and seaweed extracts increase immune parameters and resistance to pathogens. Here, we constructed the expression vector pET28b-LvLGBP and transferred it into E.coli BL21 (DE3) for protein expression and to produce the recombinant protein LGBP (rLvLGBP) in white shrimp Litopenaeus vannamei. We examined the binding of rLvLGBP with seaweed-derived polysaccharides including alginate, carrageenan, fucoidan, laminarin, Gracilaria tenuistipitata extract (GTE), and Sargassum duplicatum extract (SDE), and examined the phenoloxidase activity of shrimp haemocytes incubated with a mixture of rLvLGBP and each polysaccharide. We also examined the binding of rLvLGBP with LPS and βG, and the phenoloxidase activity of shrimp haemocytes incubated with a mixture of rLvLGBP and LPS (rLvLGBP-LPS) or a mixture of rLvLGBP and βG (rLvLGBP-βG). An ELISA binding assay indicated that rLvLGBP binds to LPS, βG, alginate, carrageenan, fucoidan, laminarin, GTE, and SDE with dissociation constants of 0.1138∼0.1770 μM. Furthermore, our results also indicated that the phenoloxidase activity of shrimp haemocytes incubated with a mixture of rLvLGBP and LPS, βG, alginate, carrageenan, fucoidan, laminarin, GTE, and SDE significantly increased by 328%, 172%, 200%, 213%, 197%, 194%, 191%, and 197 %, respectively compared to controls (cacodylate buffer). We conclude that LvLGBP functions as a PRP, recognizes and binds to LPS, βG, alginate, carrageenan, fucoidan, laminarin, GTE, and SDE, and subsequently leads to activating innate immunity in shrimp.

Keywords:
Litopenaeus vannamei, Lipopolysaccharide and β-1,3-glucan-binding protein (LGBP), Recognition, Seaweed polysaccharide, Phenoloxidase
12. 龔紘毅*、胡紹揚、吳金洌 (Hong-Yi Gong, Shao-Yang Hu, Jen-Leih Wu), "基因轉殖螢光觀賞魚發展及其不孕技術開發(Progress of transgenic fluorescent ornamental fish and development of its infertility control technologies)," 農業生技產業季刊 (Agricultural Biotechnology Industry Quarterly) 43: 34-48。, Dec, 2015.
大綱:
基因轉殖螢光觀賞魚起源及產業化介紹 (Introduction of origin and industrialization of transgenic fluorescent ornamental fish)

基因轉殖螢光觀賞魚關鍵技術 (Key technologies of transgenic fluorescent ornamental fish)
(一)基因轉殖技術 (Gene transfer technology)
(二)啟動子及增強子表現技術 (Expression technology driven by promoter and enhancer)
(三)新穎螢光蛋白基因開發 (Discovery of novel fluorescent protein genes)
(四)新品種螢光觀賞魚開發 (Development of new fluorescent ornamental fish species)
(五)不孕控制技術 (Infertility control technology)

基因轉殖魚誘導性不孕技術 (Inducible infertility control technologies in transgenic fish)
(一)利用生殖細胞專一性啟動子表現誘發性毒性蛋白抑制生殖腺發育與功能 (Expression of inducible toxin protein by germ cell-specific promoter to inhibit gonad development and function)
(二)誘導性啟動子誘發破壞原始生殖細胞(PGCs)遷移而導致不孕 (Disruption of PGCs migration leads to infertility driven by Inducible promoter)
(三)Cre/loxP與Gal4/UAS之誘導性不孕系統 (Cre/loxP and Gal4/UAS inducible infertility systems)

臺灣在螢光觀賞魚產業的關鍵角色 (Taiwan plays a key role in fluorescent ornamental fish industry)
13. Hsin-I Liu, Pinwen Peter Chiou, Hong-Yi Gong and Hsin-Yiu Chou*, "Cloning of the Major Capsid Protein (MCP) of Grouper Iridovirus of Taiwan (TGIV) and Preliminary Evaluation of a Recombinant MCP Vaccine against TGIV," International Journal of Molecular Sciences 16: 28647–28656. (IF: 2.862), Dec, 2015.
14. Cheng CL, Huang SJ, Wu CL, Gong HY, Ken CF, Hu SY, Wu JL., "Transgenic Expression of Omega-3 PUFA Synthesis Genes Improves Zebrafish Survival during Vibrio vulnificus Infection," Journal of Biomedical Science 22:103., Nov, 2015.
BACKGROUND:
Highly desaturated n-3 polyunsaturated fatty acids (PUFAs), such as eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), are synthesized by desaturases and elongase. They exert hepatoprotective effects to prevent alcoholic fatty liver syndrome or cholestatic liver injury. However, it is unclear how n-3 PUFAs improve immune function in liver. Vibrio vulnificus, a gram-negative bacterial pathogen, causes high mortality of aquaculture fishes upon infection. Humans can become infected with V. vulnificus through open wounds or by eating raw seafood, and such infections may result in systemic septicemia. Moreover, patients with liver diseases are vulnerable to infection, and are more likely than healthy persons to present with liver inflammation following infection. This study quantified n-3 PUFAs and their anti-bacterial effects in Fadsd6 and Elvol5a transgenic zebrafish.

RESULTS:
Two transgenic zebrafish strains with strong liver specific expression of Fadsd6 and Elvol5a (driven by the zebrafish Fabp10 promoter) were established using the Tol2 system. Synthesis of n-3 PUFAs in these strains were increased by 2.5-fold as compared to wild type (Wt) fish. The survival rate in 24 h following challenge with V. vulnificus was 20 % in Wt, but 70 % in the transgenic strains. In addition, the bacteria counts in transgenic fish strains were significantly decreased. The expression levels of pro-inflammatory genes, such as TNF-α, IL-1β, and NF-κB, were suppressed between 9 and 12 h after challenge. This study confirms the anti-bacterial function of n-3 PUFAs in a transgenic zebrafish model.

CONCLUSIONS:
Fadsd6 and Elvol5a transgenic zebrafish are more resistant to V. vulnificus infection, and enhance survival by diminishing the attendant inflammatory response.
15. Jenn-Kan Lu*, Hsin-Yiu Chou, Hong-Yi Gong, Pinwen P. Chiou, Chang-Wen Huang, Li-Li Chen, Jen-Leih Wu, "The Marine Aquaculture Biotechnology Status and its Sustainability in Taiwan," Current Biotechnology (2015) 4(3): 311-318., Oct, 2015.
Background: Aquaculture faces many challenges over the next decade, notably, combating diseases andepizootics, broodstock improvement and domestication, development of appropriate feeds and feeding mechanisms, hatchery and grow-out technology, as well as water-quality management. These all present considerable scope for biotechnological and other technology interventions. Aquaculture biotechnology can be described as the scientific application of biological concepts that enhance the productivity and economic viability of its various industrial sectors (Liao and Chao, 1997) [71].
Objective: This paper is to introduce the conception of sustainable aquaculture, status, objective and how to enter the way of sustainability in Taiwan. The paper provides an overview of the current trends in marine aquaculture and highlights how the Taiwan aquaculturists employed various technological innovations that occurred in the management and production of aquatic resources. The purpose of the paper is to explain the role technological innovation played in meeting the growing worldwide consumer demand for aquatic species, and in dealing with scientific uncertainty regarding the potential negative impact of these advances on the aquatic environment, fish health and the socioeconomic circumstances.

Methods: We use the OECD definition of biotechnology: “The application of science and technology to living organisms, as well as parts, products and models thereof, to alter living or non-living materials for the production of knowledge, goods and services”. To revolutionize aquaculture, it is essential to apply modern biotechnology techniques. Taiwan aquaculturists conducting biotechnology research would adopt at least one of six technologies or processes, biotechnological techniques include (a) water environmental bioremediation technology, (b) -omics, bioinformatics and molecular breeding program to select superior aquatic seeds, (c) molecular nutrition and feed biotechnology to develop functional feed and additives, and(d) biosecurity management strategies for aquatic animals, (e) smart aquaculture facility ; including recirculation system and aquaponics system, and (f) application of ICT and IoT technology to establish national wide seafood traceability system.

Results: We find it useful to follow up on this overall definition, and elaborate on its practical applications within the marine culture biotechnology sector. One way of following up is to divide into six different researches and business subareas, as follows:

a) Microbial agents for environmental remediation, bio-control and probiotics

b) New genetic markers and breeding program to speed up the selection of new strain with good traits for industrial use;

c) To develop green functional feed additives by nutrigenomics and molecular nutrition concepts, economical alternatives to fish meal as a protein source in aquaculture feeds

d) Providing new techniques for biosecurity management of aquatic species;

e) Seafood traceability to ensuring bio-safety and food safety of aquaculture and fisheries;

f) Smart aquaculture facility, including recirculation system and aquaponics system

Conclusions: The paper briefly reports the current progress in Taiwan and thrust areas in the use of probiotics for water environmental remediation, molecular markers for fish breeding and production of monosex and transgenesis, biotechnology in aquaculture nutrition and health management, gene banking, intelligent aquaculture facility and the seafood traceability system. The conclusions recapitulate some of the findings gained in this interdisciplinary analysis on the role of marine culture biotechnology in the management of aquatic resources, and emphasize the long-run perspective of technological innovation in fish and shellfish production.
16. Chung WJ, Huang CL, Gong HY, Ou TY, Hsu JL, Hu SY*, "Recombinant production of biologically active giant grouper (Epinephelus lanceolatus) growth hormone from inclusion bodies of Escherichia coli by fed-batch culture.," Protein Expression and Purification (2015) 110C: 79-88., (IF: 1.43), Feb, 2015.
Growth hormone (GH) performs important roles in regulating somatic growth, reproduction, osmoregulation, metabolism and immunity in teleosts, and thus, it has attracted substantial attention in the field of aquaculture application. Herein, giant grouper GH (ggGH) cDNA was cloned into the pET28a vector and expressed in Shuffle® T7 Competent Escherichia coli. Recombinant N-terminal 6× His-tagged ggGH was produced mainly in insoluble inclusion bodies; the recombinant ggGH content reached 20% of total protein. For large-scale ggGH production, high-cell density E. coli culture was achieved via fed-batch culture with pH-stat. After 30 h of cultivation, a cell concentration of 41.1 g/l dry cell weight with over 95% plasmid stability was reached. Maximal ggGH production (4.0 g/l; 22% total protein) was achieved via mid-log phase induction. Various centrifugal forces, buffer pHs and urea concentrations were optimized for isolation and solubilization of ggGH from inclusion bodies. Hydrophobic interactions and ionic interactions were the major forces in ggGH inclusion body formation. Complete ggGH inclusion body solubilization was obtained in PBS buffer at pH 12 containing 3 M urea. Through a simple purification process including Ni-NTA affinity chromatography and refolding, 5.7 mg of ggGH was obtained from 10 ml of fed-batch culture (45% recovery). The sequence and secondary structure of the purified ggGH were confirmed by LC–MS/MS mass spectrometry and circular dichroism analysis. The cell proliferation-promoting activity was confirmed in HepG2, ZFL and GF-1 cells with the WST-1 colorimetric bioassay.
17. Thomas T. Chen*, Chun-Mean Lin, Maria J. Chen, Jay H. Lo, Pinwen Peter Chiou, Hong-Yi Gong, Jen-Leih Wu, Mark Hung-Chih Chen, and Charlie Yarish, "Chapter 13. Transgenic Technology in Marine Organisms," Springer Handbook of Marine Biotechnology. 2015, pp387-412. Kim, Se-Kwon (Ed.). 1560p., ISBN: 978-3-642-53970-1 (Print) 978-3-642-53971-8 (Online), Jan, 2015.
18. Wang YD, Huang SJ, Chou HN, Liao WL, Gong HY, Chen JY*., "Transcriptome analysis of the effect of Vibrio alginolyticus infection on the innate immunity-related complement pathway in Epinephelus coioides.," BMC Genomics. (2014) 15(1):1102., (IF: 4.04), Dec, 2014.
BACKGROUND:
Orange-spotted grouper (Epinephelus coioides) with protogynous hermaphroditic features are one of the most economically important aquaculture species in Taiwan. However, larvae stage grouper are susceptible to infection by the bacterial pathogen Vibrio alginolyticus. To better understand the molecular mechanisms of the immune response to V. alginolyticus in Epinephelus coioides larvae, we used high-throughput deep sequencing technology to study the effect of infection on gene expression.

RESULTS:
A total of 114,851,002 reads were assembled, consisting of 9,687,355,560 nucleotides; these were further assembled into 209,082 contigs with a mean length of 372 bp. Gene ontology (GO) analysis of the transcriptome revealed 12 cellular component subcategories, 16 molecular function subcategories, and 42 biological process subcategories (P value
19. Huang SH*, Huang KS, Yu CH, and Gong HY, "Metabolic profile analysis of a single developing zebrafish embryo via monitoring of oxygen consumption rates within a microfluidic device," Biomicrofluidics (2013) 7: 064107., (IF: 3.771; BIOCHEMICAL RESEARCH METHODS, 18/78; Nanoscience & Nanotechnology, 22/30), Dec, 2013.
A combination of a microfluidic device with a light modulation system was developed to detect the oxygen consumption rate (OCR) of a single developing zebrafish embryo via phase-based phosphorescence lifetime detection. The microfluidic device combines two components: an array of glass microwells containing Pt(II) octaethylporphyrin as an oxygen-sensitive luminescent layer and a microfluidic module with pneumatically actuated glass lids above the microwells to controllably seal the microwells of interest. The total basal respiration (OCR, in pmol O2/min/embryo) of a single developing zebrafish embryo inside a sealed microwell has been successfully measured from the blastula stage (3 h post-fertilization, 3 hpf) through the hatching stage (48 hpf). The total basal respiration increased in a linear and reproducible fashion with embryonic age. Sequentially adding pharmacological inhibitors of bioenergetic pathways allows us to perform respiratory measurements of a single zebrafish embryo at key developmental stages and thus monitor changes in mitochondrial function in vivo that are coordinated with embryonic development. We have successfully measured the metabolic profiles of a single developing zebrafish embryo from 3 hpf to 48 hpf inside a microfluidic device. The total basal respiration is partitioned into the non-mitochondrial respiration, mitochondrial respiration, respiration due to ATP turnover, and respiration due to proton leak. The changes in these respirations are correlated with zebrafish embryonic development stages. Our proposed platform provides the potential for studying bioenergetic metabolism in a developing organism and for a wide range of biomedical applications that relate mitochondrial physiology and disease.
20. Li YH, Chen HY, Li YW, Wu SY, Liu W, Lin GH, Hu SY, Chang ZK, Gong HY, Liao CH, Chiang KY, Huang CW, and Wu JL.*, "Progranulin regulates zebrafish muscle growth and regeneration through maintaining the pool of myogenic progenitor cells.," Scientific Reports 3: 1176., (IF: 5.078; Multidisciplinary Sciences, 5/55), Jan, 2013.
Myogenic progenitor cell (MPC) is responsible for postembryonic muscle growth and regeneration. Progranulin (PGRN) is a pluripotent growth factor that is correlated with neuromuscular disease, which is characterised by denervation, leading to muscle atrophy with an abnormal quantity and functional ability of MPC. However, the role of PGRN in MPC biology has yet to be elucidated. Here, we show that knockdown of zebrafish progranulin A (GrnA) resulted in a reduced number of MPC and impaired muscle growth. The decreased number of Pax7-positive MPCs could be restored by the ectopic expression of GrnA or MET. We further confirmed the requirement of GrnA in MPC activation during muscle regeneration by knockdown and transgenic line with muscle-specific overexpression of GrnA. In conclusion, we demonstrate a critical role for PGRN in the maintenance of MPC and suggest that muscle atrophy under PGRN loss may begin with MPC during postembryonic myogenesis.
21. Huang CW, Li YH, Hu SY, Chi JR, Lin GH, Lin CC, Gong HY, Chen RH, Chang SJ, Liu FG, and Wu JL*., "Differential expression patterns of growth-related microRNAs in the skeletal muscle of Nile tilapia (Oreochromis niloticus).," Journal of Animal Science 90(12):4266-4279., (IF=1.92; AGRICULTURE, DAIRY & ANIMAL SCIENCE, 6/51), Dec, 2012.
MicroRNA (miRNA) are a class of small, single-stranded, non-coding RNA that regulate mRNA expression at the post-transcriptional level and play important roles in many fundamental biological processes. There is emerging evidence that miRNA are critical regulators of widespread cellular functions, such as differentiation, proliferation, and migration. At present, little is known about miRNA expression profiles related to skeletal muscle growth in aquatic organisms. This study aimed to investigate the phenotypic variation in the body growth of the Nile tilapia (Oreochromis niloticus) and to identify and quantify the differential expression levels of selected growth-related transcriptomic miRNA in the skeletal muscle of this fish. To this end, we performed next-generation sequencing to define the full miRNA transcriptome in muscle tissue from Nile tilapia and to detect differentially expressed miRNA between 2 strains of Nile tilapia. These tilapia strains exhibited significant (P< 0.05) phenotypic variation with respect to growth-related traits (body length and BW), mitochondrial DNA (mtDNA) haplotype diversity, and the differential expression of selected growth-related genes. The results obtained from the transcriptome analysis and real-time quantitative reverse transcription PCR (qRT-PCR) revealed significant differences in miRNA expression between fast-growing and control strains of tilapia. Digital gene expression (DGE) profiling was performed based on the obtained read abundance, and we identified down-regulated miRNA, including let-7j, miR-140, miR-192, miR-204, miR-218a, miR-218b, miR-301c, and miR-460, and up-regulated miRNA, including let-7b, let-7c, miR-133, miR-152, miR-15a, miR-193a, miR-30b, and miR-34, associated with body growth in tilapia. These results were further validated using real-time qRT-PCR and microarray profiling. In summary, the up- and down-regulation of miRNA involved in the GH/IGF-1 axis signaling pathway suggests that the differential expression levels of growth-related miRNA may serve as molecular markers that are predictive of specific functional and diagnostic implications. The obtained data on genetic polymorphisms in miRNA-target interactions are particularly useful for Nile tilapia breeding programs.
22. Liu W, Chen JR, Hsu CH, Li YH, Chen YM, Lin CY, Huang SJ, Chang ZK, Chen YC, Lin CH, Gong HY, Lin CC, Kawakami K, and Wu JL*, "A zebrafish model of intrahepatic cholangiocarcinoma by dual expression of hepatitis B virus X and hepatitis C virus core protein in liver.," Hepatology 56(6):2268-2276., (IF= 11.190; GASTROENTEROLOGY & HEPATOLOGY, 3/74), Dec, 2012.
The mechanisms that mediate the initiation and development of intrahepatic cholangiocarcinoma (ICC) associated with hepatitis B and C virus (HBV and HCV, respectively) infection remain largely unclear. In this study, we conditionally co-expressed hepatitis B virus X (HBx) and hepatitis C virus core (HCP) proteins in zebrafish livers, which caused fibrosis and consequently contributed to ICC formation at the age of 3 months. Suppressing the transgene expression by doxycycline (Dox) treatment resulted in the loss of ICC formation. The biomarker networks of zebrafish ICC identified by transcriptome sequencing and analysis were also frequently involved in the development of human neoplasms. The profiles of potential biomarker genes of zebrafish ICC were similar to those of human cholangiocarcinoma. Our data also showed that the pSmad3L oncogenic pathway was activated in HBx and HCP-induced ICC and included phosphorylation of p38 mitogen-activated proteinbase (MAPK) and p44/42 mitogen-activated protein kinase (ERK1/2), indicating the association with transforming growth factor beta 1 (TGF-β1) signaling pathway in ICC. Bile duct proliferation, fibrosis, and ICC were markedly reduced by knockdown of TGF-β1 by in vivo morpholinos injections. Conclusion: These results reveal that TGF-β1 plays an important role in HBx- and HCP-induced ICC development. This in vivo model is a potential approach to study the molecular events of fibrosis and ICC occurring in HBV and HCV infection.
23. Wang WL, Liu W, Gong HY, Hong JR, Lin CC, Wu JL., "Activation of cytokines expression occurs through the TNFα/NF-κB-mediated pathway in birnavirus infected cells.," Fish & Shellfish Immunology 31(1):10-21., (IF= 3.034; MARINE & FRESHWATER BIOLOGY, 10/102; VETERINARY SCIENCES, 3/129; IMMUNOLOGY, 65/144), Jul, 2011.
The infectious pancreatic necrosis virus (IPNV) belongs to the Birnaviridae family of viruses and causes acute contagious diseases in a number of economically important freshwater and marine fish. In this study, we infected zebrafish embryonic cells (ZF4) with IPNV and analyzed the gene expression patterns of normal and infected cells using quantitative real-time PCR. We identified a number of immune response genes, including ifna, ifng, mx, irf1, irf2, irf4, tnfa, tnfb, il-1b, il-15, il-26, ccl4 and mmp family genes, that are induced after viral infection. Transcriptional regulators, including cebpb, junb, nfkb and stat1, stat4 and stat5, were also upregulated in IPNV-infected cells. In addition, we used Pathway Studio software to identify TNFα as having the greatest downstream influence among these altered genes. Treating virus-infected cells with an siRNA targeting TNFα inhibited NF-κB expression. To further interrupt the TNFα/NF-κB-mediated pathway, the expression levels of cytokines and metalloproteinases were inhibited in IPNV-infected cells. These data suggest that, during IPNV infection, the expression of cytokines and metalloproteinases might be initiated through the TNFα/NF-κB-mediated pathway. The modulation of TNFα/NF-κB-related mechanisms may provide a therapeutic strategy for inhibiting viral infection in teleosts.
24. Wang WL, Hong JR, Lin GH, Liu W, Gong HY, Lu MW, Lin CC, Wu JL., "Stage-specific expression of TNFα regulates bad/bid-mediated apoptosis and RIP1/ROS-mediated secondary necrosis in Birnavirus-infected fish cells.," PLoS One. 6(2):e16740., (IF= 3.534; MULTIDISCIPLINARY SCIENCES, 8/55), Feb, 2011.
Infectious pancreatic necrosis virus (IPNV) can induce Bad-mediated apoptosis followed by secondary necrosis in fish cells, but it is not known how these two types of cell death are regulated by IPNV. We found that IPNV infection can regulate Bad/Bid-mediated apoptotic and Rip1/ROS-mediated necrotic death pathways via the up-regulation of TNFα in zebrafish ZF4 cells. Using a DNA microarray and quantitative RT-PCR analyses, two major subsets of differentially expressed genes were characterized, including the innate immune response gene TNFα and the pro-apoptotic genes Bad and Bid. In the early replication stage (0-6 h post-infection, or p.i.), we observed that the pro-inflammatory cytokine TNFα underwent a rapid six-fold induction. Then, during the early-middle replication stages (6-12 h p.i.), TNFα level was eight-fold induction and the pro-apoptotic Bcl-2 family members Bad and Bid were up-regulated. Furthermore, specific inhibitors of TNFα expression (AG-126 or TNFα-specific siRNA) were used to block apoptotic and necrotic death signaling during the early or early-middle stages of IPNV infection. Inhibition of TNFα expression dramatically reduced the Bad/Bid-mediated apoptotic and Rip1/ROS-mediated necrotic cell death pathways and rescued host cell viability. Moreover, we used Rip1-specific inhibitors (Nec-1 and Rip1-specific siRNA) to block Rip1 expression. The Rip1/ROS-mediated secondary necrotic pathway appeared to be reduced in IPNV-infected fish cells during the middle-late stage of infection (12-18 h p.i.). Taken together, our results indicate that IPNV triggers two death pathways via up-stream induction of the pro-inflammatory cytokine TNFα, and these results may provide new insights into the pathogenesis of RNA viruses.
25. Hu SY, Liao CH, Lin YP, Li YH, Gong HY, Lin GH, Kawakami K, Yang TH, Wu JL., "Zebrafish eggs used as bioreactors for the production of bioactive tilapia insulin-like growth factors.," Transgenic Research. 20(1):73-83., (IF= 2.281; BIOTECHNOLOGY & APPLIED MICROBIOLOGY, 75/165), Feb, 2011.
Multiple advantages-including the short generation time, large numbers of fertilized eggs, low cost of cultivation and easy maintenance favor the use of fish as bioreactors for the production of pharmaceutical proteins. In the present study, zebrafish eggs were used as bioreactors to produce mature tilapia insulin-like growth factors (IGFs) proteins using the oocyte-specific zona pellucida (zp3) promoter. The chimeric expression plasmids, pT2-ZP-tIGFs-IRES-hrGFP, in which hrGFP was used as reporter of tilapia IGFs expression, were designed to established Tg (ZP:tIGFs:hrGFP) transgenic lines for the expression of tilapia IGF-1 and IGF-2. Recombinant tilapia IGF-1 and IGF-2 were expressed as soluble forms in cytoplasm of fertilized eggs. The content level of tilapia IGF-1 and IGF-2 were 6.5 and 5.0% of the soluble protein, respectively. Using a simple Ni-NTA affinity chromatography purification process, 0.58 and 0.49 mg of purified tilapia IGF-1 and IGF-2 were obtained, respectively, from 650 fertilized eggs. The biological activity of the purified tilapia IGF-1 and IGF-2 was confirmed via a colorimetric bioassay to monitor the growth stimulation of zebrafish embryonic cells (ZF4), tilapia ovary cells (TO-2) and human osteosarcoma epithelial cells (U2OS). These results demonstrate that the use of zebrafish eggs as bioreactors is a promising approach for the production of biological recombinant proteins.
26. Li YH, Chen MH, Gong HY, Hu SY, Li YW, Lin GH, Lin CC, Liu W, Wu JL., "Progranulin A-mediated MET signaling is essential for liver morphogenesis in zebrafish.," Journal of Biological Chemistry. 285(52):41001-9., (IF= 4.600; BIOCHEMISTRY & MOLECULAR BIOLOGY, 65/291), Dec, 2010.
The mechanism that regulates embryonic liver morphogenesis remains elusive. Progranulin (PGRN) is postulated to play a critical role in regulating pathological liver growth. Nevertheless, the exact regulatory mechanism of PGRN in relation to its functional role in embryonic liver development remains to be elucidated. In our study, the knockdown of progranulin A (GrnA), an orthologue of mammalian PGRN, using antisense morpholinos resulted in impaired liver morphogenesis in zebrafish (Danio rerio). The vital role of GrnA in hepatic outgrowth and not in liver bud formation was further confirmed using whole-mount in situ hybridization markers. In addition, a GrnA deficiency was also found to be associated with the deregulation of MET-related genes in the neonatal liver using a microarray analysis. In contrast, the decrease in liver size that was observed in grnA morphants was avoided when ectopic MET expression was produced by co-injecting met mRNA and grnA morpholinos. This phenomenon suggests that GrnA might play a role in liver growth regulation via MET signaling. Furthermore, our study has shown that GrnA positively modulates hepatic MET expression both in vivo and in vitro. Therefore, our data have indicated that GrnA plays a vital role in embryonic liver morphogenesis in zebrafish. As a result, a novel link between PGRN and MET signaling is proposed.
27. Hu SY, Lin PY, Liao CH, Gong HY, Lin GH, Kawakami K, Wu JL., "Nitroreductase-mediated gonadal dysgenesis for infertility control of genetically modified zebrafish.," Marine Biotechnology (NY). 12(5):569-78., (IF= 3.152; MARINE & FRESHWATER BIOLOGY, 8/102), Oct, 2010.
Genetically modified (GM) fish with desirable features such as rapid growth, disease resistance, and cold tolerance, among other traits, have been established in aquaculture. However, commercially available GM fish are restricted because of global concerns over the incomplete assessments of food safety and ecological impact. The ecological impact concerns include gene flow and escape of the GM fish, which may cause extinction of wild natural fish stocks. Infertility control is a core technology for overcoming this obstacle. Although polyploidy technology, GnRH-specific antisense RNA, and RNAi against GnRH gene expression have been used to cause infertility in fish, these approaches are not 100% reliable and are not heritable. In the present study, zebrafish was used as a model to establish an inducible platform of infertility control in GM fish. Nitroreductase, which converts metronidazole substrate into cytotoxin, was fused with EGFP and expressed specifically by oocytes in the Tg(ZP:NTR-EGFP) by a zona pellucida promoter. Through consecutive immersion of metronidazole from 28 to 42 days posthatching, oocyte-specific EGFP expression was eliminated, and atrophy of the gonads was detected by anatomical analysis. These findings reveal that oocyte-specific nitroreductase-mediated catalysis of metronidazole blocks oogenesis and leads to an undeveloped oocyte. Furthermore, oocyte cell death via apoptosis was detected by a TUNEL assay. We found that the gonadal dysgenesis induced by metronidazole resulted in activation of the ovarian killer gene bok, which is a proapoptotic gene member of the Bcl-2 family and led to infertility. These results show that oocyte-specific nitroreductase-mediated catalysis of metronidazole can cause reliable infertility in zebrafish and could potentially be used as a model for other aquaculture fish species.
28. Lee CY, Hu SY, Gong HY, Chen MH, Lu JK, Wu JL., "Suppression of myostatin with vector-based RNA interference causes a double-muscle effect in transgenic zebrafish.," Biochem Biophys Res Commun. 387(4):766-71., (IF= 2.281; BIOCHEMISTRY & MOLECULAR BIOLOGY, 191/291), Oct, 2009.
Myostatin belongs to the transforming growth factor (TGF)-beta superfamily and is a potent negative regulator of skeletal muscle development and growth. We utilized microinjection of an antisense RNA-expressing vector to establish a hereditarily stable myostatin gene knockdown zebrafish strain with a double-muscle phenotype. Real-time PCR and immunostaining revealed that the myostatin messenger (m)RNA and protein levels in homozygous transgenic zebrafish were 33% and 26% those of the non-transgenic controls, respectively. Also, the mRNA levels of myogenic regulatory factor markers such as MyoD, myogenin, Mrf4, and Myf5 were dramatically elevated in myostatin-suppressed transgenic fish compared to the non-transgenic controls. Although there was no significant difference in body length, homozygous transgenic zebrafish were 45% heavier than non-transgenic controls. Histochemical analysis showed that the cross-sectional area of the muscle fiber of homozygous transgenic fish was twice as large as that of non-transgenic controls. This is the first model zebrafish with a hereditarily stable myostatin-suppressed genotype and a double-muscle phenotype.
29. Hu SY, Chen MH, Lin YC, Lin GH, Gong HY, Yang TH, Wu JL., "Cloning and functional analysis of the proximal promoter region of the three GnRH genes from the silver sea bream (Sparus sarba).," Comparative Biochemistry and Physiology B-Biochem Mol Biol. 151(4):373-80., (IF= 1.904; ZOOLOGY, 30/152), Dec, 2008.
Gonadotropin-releasing hormone (GnRH) is a neuropeptide that plays a major role in releasing pituitary gonadotropin and controlling vertebrate reproduction. In this study, three GnRH cDNAs, GnRH-I (sbGnRH; 348 bp), GnRH-II (cGnRH-II; 557 bp), and GnRH-III (sGnRH; 483 bp), were cloned from the brain of the silver sea bream (Sparus sarba). In order to understand how the expression of the GnRH isoforms was regulated in the brain, the promoter of each gene was cloned and analyzed. We found regulatory motifs in the promoters that were conserved in the GnRH promoters of tilapia and zebrafish, suggesting that these motifs play a critical role in GnRH regulation. We performed functional analyses and examined tissue-specific expression for each GnRH promoter using EGFP reporter fusions in zebrafish. The GnRH-I promoter was active in the forebrain area, including the olfactory bulb-terminal nerve area and peripheral preoptic areas; the GnRH-II promoter was active in the midbrain; and the GnRH-III promoter was active in the olfactory bulb. These results show that the GnRH promoters of the silver sea bream GnRH genes exhibit tissue-specific activity.
30. Lin CJ, Gong HY, Tseng HC, Wang WL, Wu JL., "miR-122 targets an anti-apoptotic gene, Bcl-w, in human hepatocellular carcinoma cell lines.," Biochem Biophys Res Commun. 375(3):315-20., (IF= 2.281; BIOCHEMISTRY & MOLECULAR BIOLOGY, 191/291), Oct, 2008.
miR-122, a hepato-specific microRNA (miRNA), is frequently down-regulated in human hepatocellular carcinoma (HCC). In an effort to identify novel miR-122 targets, we performed an in silico analysis and detected a putative binding site in the 3'-untranslated region (3'-UTR) of Bcl-w, an anti-apoptotic Bcl-2 family member. In the HCC-derived cell lines, Hep3B and HepG2, we confirmed that miR-122 modulates Bcl-w expression by directly targeting binding site within the 3'-UTR. The cellular mRNA and protein levels of Bcl-w were repressed by elevated levels of miR-122, which subsequently led to reduction of cell viability and activation of caspase-3. Thus, Bcl-w is a direct target of miR-122 that functions as an endogenous apoptosis regulator in these HCC-derived cell lines.
31. Amali AA, Lin CJ, Chen YH, Wang WL, Gong HY, Rekha RD, Lu JK, Chen TT, Wu JL., "Overexpression of Myostatin2 in zebrafish reduces the expression of dystrophin associated protein complex (DAPC) which leads to muscle dystrophy.," J Biomed Sci. 15(5):595-604., (IF= 2.736; MEDICINE, RESEARCH & EXPERIMENTAL, 49/122), Sep, 2008.
Myostatin, a member of the TGF-beta superfamily, is a potent negative regulator of skeletal muscle and growth. Previously, we reported Mstn1 from zebrafish and studied its influence on muscle development. In this study, we identified another form of Myostatin protein which is referred to as Mstn2. The size of Mstn2 cDNA is 1342 bp with 109 and 132 bp of 5' and 3'-untranslated regions (UTRs), respectively. The coding region is 1101 bp encoding 367 amino acids. The identity between zebrafish Mstn1 and 2 is 66%. The phylogenetic tree revealed that the Mstn2 is an ancestral form of Mstn1. To study the functional aspects, we overexpressed mstn2 and noticed that embryos became less active and the juveniles with bent and curved phenotypes when compared to the control. The RT-PCR and in situ hybridization showed concurrent reduction of dystrophin associated protein complex (DAPC). In cryosection and in situ hybridization, we observed the disintegration of somites, lack of transverse myoseptum and loss of muscle integrity due to the failure of muscle attachment in mstn2 overexpressed embryos. Immunohistochemistry and western blot showed that there was a reduction of dystrophin, dystroglycan and sarcoglycan at translational level in overexpressed embryos. Taken together, these results indicate the suitability of zebrafish as an excellent animal model and our data provide the first in vivo evidence of muscle attachment failure by the overexpression of mstn2 and it leads to muscle loss which results in muscle dystrophy that may contribute to Duchenne syndrome and other muscle related diseases.
32. Wu HC, Chiu CS, Wu JL, Gong HY, Chen MC, Lu MW, Hong JR., "Zebrafish anti-apoptotic protein zfBcl-xL can block betanodavirus protein alpha-induced mitochondria-mediated secondary necrosis cell death.," Fish & Shellfish Immunology. 24(4):436-49., (IF= 3.034; MARINE & FRESHWATER BIOLOGY, 10/102; VETERINARY SCIENCES, 3/129; IMMUNOLOGY, 65/144), Apr, 2008.
Betanodavirus protein alpha induces cell apoptosis or secondary necrosis by a poorly understood process. In the present work, red spotted grouper nervous necrosis virus (RGNNV) RNA 2 was cloned and transfected into tissue culture cells (GF-1) which then underwent apoptosis or post-apoptotic necrosis. In the early apoptotic stage, progressive phosphatidylserine externalization was evident at 24h post-transfection (p.t.) by Annexin V-FLUOS staining. TUNEL assay revealed apoptotic cells at 24-72 h p.t, after which post-apoptotic necrotic cells were identified by acridine orange/ethidium bromide dual dye staining from 48 to 72 h p.t. Protein alpha induced progressive loss of mitochondrial membrane potential (MMP) which was detected in RNA2-transfected GF-1 cells at 24, 48, and 72 h p.t., which correlated with cytochrome c release, especially at 72 h p.t. To assess the effect of zfBcl-xL on cell death, RNA2-transfected cells were co-transfected with zfBcl-x(L). Co-transfection of GF-1 cells prevented loss of MMP at 24 h and 48 h p.t. and blocked initiator caspase-8 and effector caspase-3 activation at 48 h p.t. We conclude that RGNNV protein alpha induces apoptosis followed by secondary necrotic cell death through a mitochondria-mediated death pathway and activation of caspases-8 and -3.
33. Rekha RD, Amali AA, Her GM, Yeh YH, Gong HY, Hu SY, Lin GH, Wu JL., "Thioacetamide accelerates steatohepatitis, cirrhosis and HCC by expressing HCV core protein in transgenic zebrafish Danio rerio.," Toxicology. 243(1-2):11-22., (IF= 4.099; TOXICOLOGY: 14/87), Jan, 2008.
Hepatocellular carcinoma (HCC) is one of the common cancers worldwide, caused by Hepatitis C virus (HCV) and hepatotoxins. Here we report the development of HCC in wild type as well as HCV core protein (HCP)-transgenic zebrafish upon treatment with a hepatotoxin, thioacetamide (TAA). Two-fold accelerated HCC development could be achieved in the TAA-treated transgenic fish, that is, the progression of the disease in TAA-treated wild type zebrafish developed HCC in 12 weeks whereas that of HCP-transgenic zebrafish shortened the HCC progression to 6 weeks. Histopathological observation showed the specific pathological features of HCC. The HCC progression was confirmed through RT-PCR that revealed an up and down regulation of different marker genes at various stages of HCC progression such as, steatohepatitis, fibrosis and HCC. Moreover, HCV core protein expressed in the HCP-transgenic zebrafish and TAA synergistically accelerate the HCC development. It must be mentioned that, this is the first report revealing HCV core protein along with TAA to induce HCC in zebrafish, particularly, in a short period of time comparing to mice model. As zebrafish has already been considered as a good human disease model and in this context, this HCC-zebrafish model may serve as a powerful preclinical platform to study the molecular events in hepatocarcinogenesis, therapeutic strategies and for evaluating chemoprevention strategies in HCC.
34. Chou, M. C., Chen, J. C., Chen, J. Y.*, Gong, H. Y., Li, L. T., Huang, T. C., Wu, J. L. and Kuo, C. M., "Isolation and characterization of the zebrafish (Danio rerio) insulin-like growth factor binding protein-3 promoter region.," Fisheries Science 74: 153-166., (IF= 0.855; FISHERIES, 36/50), Jan, 2008.
Insulin-like growth factor binding protein-3 (IGFBP-3) is expressed in many different cell types and has a regulatory function in the insulin-like growth factor (IGF) system. To further understand the molecular mechanism, we cloned and sequenced the 5′-promoter region of zebrafish Danio rerio IGFBP-3 and characterized its activity using firefly luciferase transient transfection expression assays. Different fragments of the zebrafish IGFBP-3 5′-flanking region were transfected into HeLa and ZFL cells. In these cell lines, maximum promoter activity was located within 1218 bp of the zebrafish IGFBP-3 flanking region in the HeLa cell line and within 304 bp of the zebrafish IGFBP-3 flanking region in the ZFL cell line. Several putative transcription factors were revealed in the zebrafish IGFBP-3 promoter region, such as organic cation transporter-1, GATA-1 and yin and yang-1. Further study of the in vivo expression of the IGFBP-3 promoter during development was carried out in transgenic zebrafish expressing an IGFBP-3 promoter-driven green fluorescent protein (GFP) encoding the GFP cDNA transgene. The GFP transcripts appeared for the first time in the 32-cell stage. These results indicate that the IGFBP-3 promoter is active in a development-specific manner and suggest that the IGFBP-3 promoter plays an important role in teleost embryo growth. Finally, IGFBP-3 has important IGF-independent effects on cell growth and may involve nuclear localization. By transiently transfecting HeLa cells with various zebrafish IGFBP-3 segments, we identified one nuclear localization signal (NLS): a basic amino acid rich sequence (PSKGRKR) between amino acids 256 and 262 was able to direct enhanced green fluorescence protein predominantly into the nucleus, whereas a deletion of this motif abrogated this nuclear localization property. These data suggest that zebrafish IGFBP-3 contains a NLS around the second NLS sequence, whereas the putative NLS at the first NLS is non-functional.
35. Huang WT, Yu HC, Hsu CC, Liao CF, Gong HY, Lin CJ, Wu JL, Weng CF., "Steroid hormones (17beta-estradiol and hydrocortisone) upregulate hepatocyte nuclear factor (HNF)-3beta and insulin-like growth factors I and II expression in the gonads of tilapia (Oreochromis mossambicus) in vitro.," Theriogenology. 68(7):988-1002., (IF= 1.845; VETERINARY SCIENCES, 17/129), Oct, 2007.
Hepatocyte nuclear factors (HNF-1alpha, -1beta and -3beta) and insulin-like growth factors (IGF-I and -II), which are involved in liver-specific gene expression, metabolism, development and cell growth, have been found in the gonads of tilapia (Oreochromis mossambicus). However, the functions of these factors and how they interact within the gonads of bony fish are not understood. In the present study, we provided experimental evidence that the expression of HNF-3beta in the gonads of tilapia, but not HNF-1alpha and -1beta, was affected in vitro by 17beta-estradiol and hydrocortisone. Immunohistochemical staining confirmed that tilapia HNF-3beta was mainly found in the nuclei of hepatocytes, the follicular granulosa cells of the ovaries, and the interstitial cells of the testes of adult tilapia. Further data were gathered at various steroid concentrations (0.1, 1, 10, 100, and 1000 nM) over various culture intervals (6, 12, 18, 24, 30, and 36 h) and subjected to semi-quantitative RT-PCR analysis. The expression of downstream genes (IGF-I and -II) followed the same temporal patterns as HNF-3beta, albeit at decreased levels for 30 and 36 h culture intervals. Both hormones upregulated HNF-3beta mRNA expression at concentrations of 0.1-10 nM, and reached optimal physiological concentrations for induction of IGFs at 1-10 nM. The identity of the PCR fragments was concurrently verified by sequencing and PCR-Southern hybridization. We inferred that HNF-3beta and IGFs may play a regulatory role in tilapia gonads during oocyte maturation and spermatogenesis.
36. Hu MC*, Gong HY*, Lin GH, Hu SY, Chen MH, Huang SJ, Liao CF, Wu JL., "XBP-1, a key regulator of unfolded protein response, activates transcription of IGF1 and Akt phosphorylation in zebrafish embryonic cell line.," Biochem Biophys Res Commun. 359(3):778-83., * equal contribution (IF= 2.281; BIOCHEMISTRY & MOLECULAR BIOLOGY, 191/291), Aug, 2007.
The unfolded protein response (UPR) is a conserved and adaptive cellular response to increase cell survival during ER stress. XBP-1 spliced form (XBP-1S) generated by IRE1 endoribonuclease is a key transcriptional regulator in UPR to activate genes involved in protein folding and degradation to restore ER function. Although Akt activation was suggested to be a pro-survival pathway activated during ER stress, the signal to trigger Akt is still not clear. In this study, we report IGF1 transcription and Akt phosphorylation are enhanced in XBP-1S stably overexpressed clone of zebrafish embryonic cell line (ZF4). In addition, zebrafish IGF1 intron1 with predicted UPRE (XBP-1S binding sites) and ERSE (ATF6/XBP-1S binding site) linked with basal promoter could be activated by XBP-1S, not by XBP-1 unspliced form (XBP-1U). Furthermore, we demonstrate that expression of endogenous IGF1 is transiently induced as XBP-1 splicing during ER stress in parallel to ER chaperone GRP78/Hspa5 and ER resided E3 ubiquitin ligase Synoviolin in ZF4 cells by quantitative PCR. Our results suggest zebrafish XBP-1S not only activates genes responsible for protein folding, transporting, glycosylation and ER associated degradation but also activates anti-apoptosis signal via IGF1/Akt pathway in unfolded protein response to cope with ER stress.
37. Chen MH, Li YH, Chang Y, Hu SY, Gong HY, Lin GH, Chen TT, Wu JL., "Co-induction of hepatic IGF-I and progranulin mRNA by growth hormone in tilapia, Oreochromis mossambiccus.," General and Comparative Endocrinology. 150(2):212-8., (IF= 2.674; ENDOCRINOLOGY & METABOLISM, 64/123), Jan, 2007.
Like IGF-I, progranulin (pgrn) is a growth factor involved in tumorigenesis and wound healing. We report here the identification and characterization of pgrn cDNA in tilapia and the regulation of its expression by growth hormone (GH). The tilapia pgrn cDNA was cloned by RT-PCR amplification, using gene specific oligonucleotides as amplification primers. The cDNA contains an open reading frame encoding a peptide of 206 amino acid residues (aa) that contains a presumptive signal peptide (23 aa) and two repeat units of granulin (grn, 51 and 52 aa, respectively) franked by a GAP of 49 aa and the carboxyl terminus with 31 aa. The two predicted grn peptides are arranged in tandem repeats interrupted by a GAP peptide. RT-PCR analysis revealed that high levels of prgn mRNA were present in several tissues such as spleen, gastric cecum, intestine, fat tissue, gill, kidney, eye and pancreas, and lower levels in liver, muscle, heart, brain, skin and stomach. Administration of a single dose (500 ng/g body weight) of recombinant seabream growth hormone (rbGH) by intraperitoneal (ip) injection into one-month-old tilapia resulted in an obvious increase of IGF-I and pgrn mRNA (2.7-fold and 2.5-fold, respectively) in the liver at three hours post-GH treatment. The peptide levels of pgrn in the liver of GH-treated fish also were substantially induced over controls at 12h post-GH treatment as detected by western immuno-blot analysis. The co-induction of IGF-I and pgrn following GH treatment may suggest the involvement of pgrn in GH regulated growth in tilapia.
38. Hu SY, Huang, JH, Huang WT, Yeh YH, Chen MHC, Gong HY, Chiou TT, Yang TH, Chen TT, Lu JK, Wu JL., "Structure and function of antimicrobial peptide penaeidin-5 from the black tiger shrimp Penaeus monodon," Aquaculture 260: 61-68., (IF= 1.828; MARINE & FRESHWATER BIOLOGY, 37/102; FISHERIES, 11/49), Sep, 2006.
The gene for penaeidin-5, an antimicrobial peptide comprising 55 amino acids, was isolated from the hemocyte of black tiger shrimp (Penaeus monodon). RT-PCR expression tests revealed that penaeidin-5 was produced in hemocytes, gills, the intestine and muscle. Western blot analysis confirmed the panaeidin-5 was aboundantin hemocytes, the intestine and hemolymph. Immunohistochemistry revealedpenaeidin-5 in the cuticle and gills that are considered primary defense barriers. The deduced amino acid sequence of penaeidin-5 included a proline-rich N-terminal domain and a carboxyl-domain that contained six cysteine
residues. Circular dichrosim analysis revealed an α-helix in its secondary structure and the predicted 3D structure indicated two disulfide bridges in the α-helix. Based on the sequence of penaeidin-5 peptide cDNA, synthetic penaeidin-5 was prepared to carry out functional tests. The synthetic peptide had efficient bacteriostatic and bactericidal activity against Aerococcus viridans, and also inhibited the growth of two filamentous fungi, Fusarium pisi and Fusarium oxysporum. To measure penaeidin-5 in vivo, black tiger shrimp were challenged with Vibrio alginolyticus and A. viridans. At 3 h post-challenge, penaeidin-5 was induced and bacterial numbers decreased significantly by 12 h and 24 h.
39. Amali AA, Rekha RD, Lin CJ, Wang WL, Gong HY, Her GM, Wu JL., "Thioacetamide induced liver damage in zebrafish embryo as a disease model for steatohepatitis.," J Biomed Sci. 13(2):225-32., (IF= 2.736; MEDICINE, RESEARCH & EXPERIMENTAL, 49/122), Mar, 2006.
Steatohepatitis has recently been increasing as a cofactor influencing the progression of fibrosis, cirrhosis, adenoma and carcinoma in liver; however, the mechanisms by which it contributes to liver injury remain uncertain. We induced steatohepatitis in zebrafish embryos using thioacetamide (TAA). TUNEL assay revealed significant increasing of apoptosis in liver after 5 days post fertilization and the increasing of apoptosis was observed to be associated with the up-regulation of apoptotic genes such as, bad, bax, P-38a, caspase-3 and 8, and JNK-1. Histological sections by oil red O stain showed the accumulation of fatty droplets which causes the pushing of the nucleus towards one side. Up-regulation of steatosis markers such as, ACC, adiponectin, PTL, CEBP- alpha and beta, SREBP-1 was also observed. Furthermore, the elevation of glutathione peroxidase in TAA treated embryos indicated that TAA induces lipid peroxidation which leads to causes liver damage. Zebrafish has already been considered as a good human disease model and in this context; TAA-treated zebrafish may serve as a good animal model to study the molecular pathogenesis of steatohepatitis. Moreover, non-availability of specific drugs to prevent steatohepatitis, this animal model may serve as a powerful preclinical platform to study the therapeutic strategies and for evaluating chemoprevention strategies for this disease.
40. Chen JY, Chou MJ, Gong HY, Huang TC, Wu JL, Kuo CM., "Cloning and biological analysis of the zebrafish (Danio rerio) insulin-like growth factor binding protein-2 proximal promoter region.," DNA Cell Biol. 24(3):199-208., (IF= 1.991; GENETICS & HEREDITY, 109/164), Mar, 2005.
Insulin-like growth factor binding protein 2 (IGFBP-2) plays an important role in the regulation of IGF's action and endocrinology in fish. To understand the molecular mechanism which controls transcription of the IGFBP-2 gene, we cloned and sequenced the IGFBP-2 proximal promoter region of the zebrafish IGFBP-2 gene and characterized its activity by firefly luciferase transient transfection expression assays. Different fragments of the zebrafish IGFBP-2 5'-flanking region were transfected into Hela and ZFL cells. In these cell lines, maximum promoter activity was located in the 900 base pairs (bp) of the zebrafish IGFBP-2 5' flanking region in the ZFL cell line and 318 bp of the zebrafish IGFBP-2 5' flanking region in the Hela cell line. The in vivo actions of the IGFBP-2 promoter on developmental stage expression were further investigated in transgenic zebrafish in which an IGFBP-2 (900-bp) promoter-driven green fluorescent protein encoding the GFP cDNA transgene was microinjected into zebrafish embryos. Morphological and RT-PCR studies of transgenic zebrafish indicated that the IGFBP-2 promoter-driven GFP transcripts appeared for the first time in the 32-cell stage. These results indicate that the IGFBP-2 promoter is active in a development-specific manner. These results suggest that the IGFBP-2 promoter plays an important role in teleost embryo growth.
41. Gong HY, Wu JL, Huang WT, Ji-Fan Lin C, Weng CF., "Response to acute changes in salinity of two different muscle type creatine kinase isoforms, from euryhaline teleost (Oreochromis mossambicus) gills.," Biochim Biophys Acta (BBA)- General Subjects. 1675(1-3):184-91., (IF= 3.829; BIOCHEMISTRY & MOLECULAR BIOLOGY, 93/291; BIOPHYSICS, 19/74), Nov, 2004.
Two CKM isoforms (CKM1 and CKM2) from the gills of tilapia (Oreochromis mossambicus) were obtained after transfer from freshwater (FW) to seawater (SW, 25 ppt). Based on the 5' and 3' RACE, the identity of CKM1 and CKM2 was determined to be 59% in the 5'-untranslated region (5'-UTR) and 41.9% in the 3'-UTR. Using Northern blot hybridization with the CKM1 and CKM2 3'-UTR probes, CKM1 and CKM2 were found to be expressed in muscle, heart and gill. The levels of these two different CK isoforms (CKM1 and CKM2) were shown to be different in FW than after acute SW transfer, showing that CKM isoforms respond to changes in salinity.
42. Gong HY, Lin CJ, Chen MH, Hu MC, Lin GH, Zhou Y, Zon LI, Wu JL., "Two distinct teleost hepatocyte nuclear factor 1 genes, hnf1alpha/tcf1 and hnf1beta/tcf2, abundantly expressed in liver, pancreas, gut and kidney of zebrafish.," Gene. 338(1):35-46., (IF= 2.196; GENETICS & HEREDITY, 99/161), Aug, 2004.
Two distinct forms of zebrafish hepatocyte nuclear factor 1 (hnf1) were identified and referred to as hnf1alpha/tcf1 and hnf1beta/tcf2. Both hnf1 genes were shown to be expressed abundantly in liver, pancreas, gut and kidney. Zebrafish HNF1alpha and HNF1beta proteins contain all HNF1 signature domains including the dimerization domain, POU-like domain and atypical homeodomain. Sequence and phylogenetic analysis reveals that zebrafish hnf1alpha is closer to tetrapodian hnf1alpha than to tetrapodian hnf1beta and zebrafish hnf1beta is highly conserved with tetrapodian hnf1beta. Existences of hnf1alpha and hnf1beta in teleost zebrafish, tilapia and fugu suggest that hnf1 gene duplication might occur before the divergence of teleost and tetrapod ancestors. Zebrafish hnf1alpha and hnf1beta genes were mapped to linkage group LG8 and LG15 in T51 panel by RH mapping and are composed of 10 and 9 exons, respectively. Zebrafish hnf1beta gene with at least 11 genes in LG15 was identified to maintain the conserved synteny with those of human in chromosome 17 and those of mouse in chromosome 11. Our results indicate that distinct hnf1alpha and hnf1beta genes in teleosts had been evolved from the hnf1 ancestor gene of chordate.
43. Amali AA, Lin CJ, Chen YH, Wang WL, Gong HY, Lee CY, Ko YL, Lu JK, Her GM, Chen TT, Wu JL., "Up-regulation of muscle-specific transcription factors during embryonic somitogenesis of zebrafish (Danio rerio) by knock-down of myostatin-1.," Developmental Dynamics. 229(4):847-56., (IF= 2.668; ANATOMY & MORPHOLOGY, 4/20; DEVELOPMENTAL BIOLOGY, 21/41), Apr, 2004.
Myostatin, a secreted growth and differentiation factor (GDF-8) belongs to transforming growth factor (TGF-beta) superfamily that plays as a negative regulator of skeletal muscle development and growth. Recently, myostatin has been isolated from fish; however, its role in muscle development and growth remains unknown. Here, we present the expression of myostatin during development and the effects of its knock-down on various genes such as muscle regulatory transcription factors (MRFs), muscle-specific proteins (MSP), and insulin-like growth factors (IGFs). The myostatin expression was found to be maternal as it starts in one-cell stage onward. The reverse transcription-polymerase chain reaction (RT-PCR), in situ hybridization, and Southern and Northern blots demonstrated that the myostatin expression is not only restricted to skeletal muscle, but it expressed all the tested tissues. Expression of myostatin was effected by using antisense morpholinos resulted in significant phenotypic difference in stages 18 and 20 hours postfertilization (hpf). To confirm the specificity of myostatin morpholino, furthermore, a rescue experiment was conducted. The length as well as width of somites was increased with almost no gap in between the somites. In addition, it deserves to mention that this is a first animal model that shows changes in the size of the somites. Moreover, analyses of MRFs, MSP, and IGFs in the knock-down embryos by RT-PCR revealed the up-regulation of MyoD, Myogenin, and Mck transcription, whereas IGF-2 transcription showed mild response with no effect on IGF-1, Desmin, and Myf5. In situ hybridization showed that there was an increase in the number of somites from 3 to 4 at 13 and 22 hpf. Taken together, these data suggest that myostatin plays a major role during myogenesis, apart from inhibition of proliferation as well as differentiation.
44. Lin LY, Chiang CC, Gong HY, Cheng CY, Hwang PP, Weng CF., "Cellular distributions of creatine kinase in branchia of euryhaline tilapia (Oreochromis mossambicus)," Am J Physiol Cell Physiol. 284(1): C233-C241., (IF: 3.674; CELL BIOLOGY, 81/185; PHYSIOLOGY, 17/81), Jan, 2003.
Although euryhaline teleosts can adapt to environmental fluctuation of salinity, their energy source for responding to changes in salinity and osmolarity remains unclear. This study examines the cellular localization of creatine kinase (CK) expression in branchia of tilapia (Oreochromis mossambicus). Western blot analysis of muscle-type CK (MM form) revealed a high association with salinity changes, but BB and MB forms of CK in the gills of fish adapted to seawater did not change. With the use of immunocytochemistry, three CK isoforms (MM, MB, and BB) were localized in mitochondria-rich (MR) cells and other epithelial cells of tilapia gills. In addition, staining intensity of MM-form CK in MR cells increased after seawater transfer, whereas BB and MB forms did not significantly change. To our knowledge, this work presents the first evidence of CK expression in MR cells of tilapia gills, highlighting the potential role of CK in providing energy for ion transport.
45. Hong JR, Gong HY, Wu JL., "IPNV VP5, a novel anti-apoptosis gene of the Bcl-2 family, regulates Mcl-1 and viral protein expression.," Virology. 295(2):217-29., (IF= 3.278; VIROLOGY, 16/32), Apr, 2002.
VP5, a 5'-terminal, small open reading frame in segment A of the aquatic birnavirus (infectious pancreatic necrosis virus, IPNV) genome, encodes a 17-kDa nonstructural protein. We previously reported apoptosis induced by IPNV in a fish cell line. In the present study, we cloned and identified VP5 and tested its function. Comparisons of the amino acid sequence of VP5 with well-known Bcl-2 family member proteins showed that the VP5 protein contains Bcl-2 homology (BH) domains BH1, BH2, BH3, and BH4 but without the transmembrane region. VP5-stable clones enhanced viability, prevented membrane blebbing, delayed DNA internucleosomal cleavage, and decreased virus titer during IPNV infection but, when deleted, BH domains 1 and 2 could lose the preventable ability. In addition, VP5 was demonstrated to be able to enhance or assist in maintaining the functional half-life of survival factor Mcl-1 and regulate specific viral protein expression during the early replication cycle. Finally, we found that VP5 was capable of enhancing cell viability when cells were exposed to UV irradiation. In summary, these results suggest that the aquatic birnavirus may utilize a notable strategy via VP5 to regulate the host apoptosis-off system for enhancing progeny production.
Attachments:
1. IPNV-VP5-Virology-2002.pdf
46. Weng CF, Chiang CC, Gong HY, Chen MH, Lin CJ, Huang WT, Cheng CY, Hwang PP, Wu JL., "Acute changes in gill Na+-K+-ATPase and creatine kinase in response to salinity changes in the euryhaline teleost, tilapia (Oreochromis mossambicus).," Physiol Biochem Zool. 75(1):29-36., (IF= 2.050; ZOOLOGY, 25/152; PHYSIOLOGY, 46/81 ), Jan, 2002.
Some freshwater (FW) teleosts are capable of acclimating to seawater (SW) when challenged; however, the related energetic and physiological consequences are still unclear. This study was conducted to examine the changes in expression of gill Na(+)-K(+)-ATPase and creatine kinase (CK) in tilapia (Oreochromis mossambicus) as the acute responses to transfer from FW to SW. After 24 h in 25 ppt SW, gill Na(+)-K(+)-ATPase activities were higher than those of fish in FW. Fish in 35 ppt SW did not increase gill Na(+)-K(+)-ATPase activities until 1.5 h after transfer, and then the activities were not significantly different from those of fish in 25 ppt SW. Compared to FW, the gill CK activities in 35 ppt SW declined within 1.5 h and afterward dramatically elevated at 2 h, as in 25 ppt SW, but the levels in 35 ppt SW were lower than those in 25 ppt SW. The Western blot of muscle-type CK (MM form) was in high association with the salinity change, showing a pattern of changes similar to that in CK activity; however, levels in 35 ppt SW were higher than those in 25 ppt SW. The activity of Na(+)-K(+)-ATPase highly correlated with that of CK in fish gill after transfer from FW to SW, suggesting that phosphocreatine acts as an energy source to meet the osmoregulatory demand during acute transfer.
47. Weng CF, Chiang CC, Gong HY, Chen MH, Huang WT, Cheng CY, Wu JL., "Bioenergetics of adaptation to a salinity transition in euryhaline teleost (Oreochromis mossambicus) brain.," Exp Biol Med. 227(1):45-50., (IF= 2.226; MEDICINE, RESEARCH & EXPERIMENTAL, 61/121), Jan, 2002.
Freshwater (FW) teleosts are capable of acclimating to seawater (SW) following such a transfer from FW. However, their osmoregulating mechanisms are still unclear, particularly those in the brain. The present study was conducted to examine acute changes that occur in brain Na(+)-K(+)-ATPase activity, creatine kinase (CK) activity, creatine, creatinine contents, and ATP levels of tilapia (Oreochromis mossambicus) in response to this transition. After transfer to SW (25 ppt), the Na(+)-K(+)-ATPase activity was maintained for 8 hr at higher levels than that in FW. In contrast, in 35 ppt SW, Na(+)-K(+)-ATPase was maintained at a even higher level than in FW for the first 2 hr. Brain Na(+)-K(+)-ATPase contents in both the 25 and 35 ppt SW groups were significantly elevated within 1 and 0.5 hr after transfer from FW, respectively. Interestingly, brain CK activities and content (homodimer of the B subunit [BB] form) in both the 25 and 35 ppt SW groups were significantly elevated within 1 hr after transfer from FW. The ATP contents in 35 ppt SW increased abruptly within 0.5 hr, and then gradually decreased during the next 2 hr. Unlike the 35 ppt group that declined in ATP contents, the 25 ppt group leveled off within 24 hr. The elevations in CK activity and creatine levels after transfer from FW to SW imply that abrupt salinity changes alter phosphocreatine/CK ratio. Such changes are needed to satisfy the increases in the energetic requirement of the cotransport mechanisms mediating osmoregulation.
Attachments:
1. tilapia-CKB-EBM-2002.pdf
48. Huang WT, Gong HY, Lin CJ, Weng CF, Chen MH, Wu JL., "Hepatocyte nuclear factors-1alpha, -1beta, and -3beta expressed in the gonad of tilapia (Oreochromis mossambicus).," Biochem Biophys Res Commun. 288(4):833-40., IF= 2.281; BIOCHEMISTRY & MOLECULAR BIOLOGY, 191/291), Nov, 2001.
Hepatocyte nuclear factors (HNFs) are upstream regulators of many liver-specific genes and are involved in many cellular functions in the body, but their existence, expression, and function in gonads are still poorly understood. Here we report on the first cloning of partial cDNAs of HNF-1alpha and -1beta and full HNF-3beta cDNA from a tilapia (Oreochromis mossambicus) liver cDNA library. The deduced amino acid sequence of tilapia HNF-3beta has a 90 to 96% identity with those of other fishes (dwarf gourami, medaka, and zebrafish), 74% with mammals (human, rat, and mouse), and 82% with Xenopus. RT-PCR detected IGF-I and -II and HNF-1alpha, -1beta, and -3beta in both liver and gonads and the identity of the PCR fragments was confirmed by PCR hybridization. Immunoprecipitation and Western blotting also detected all three HNF proteins in both liver and gonads. Expression of HNFs in the gonads of the tilapia suggests that multi-HNFs may form a cascade to regulate gonadal physiology in the bony fish.
49. Chen MC, Gong HY, Cheng CY, Wang JP, Hong JR, Wu JL., "Cloning and characterization of zfBLP1, a Bcl-XL homologue from the zebrafish, Danio rerio.," Biochim Biophys Acta.-Gene Structure and Expression 1519(1-2):127-33., (IF= 1.704; BIOCHEMISTRY & MOLECULAR BIOLOGY, 187/263), May, 2001.
The importance of the Bcl-2 family proteins in normal vertebrate embryogenesis is being recognized; however, their regulatory mechanism is poorly understood. We report here the cloning and characterization of a novel zebrafish Bcl-2 family protein, zfBLP1. The zfBLP1 cDNA is 1942 nucleotides long, encoding a polypeptide of 238 amino acids. The primary sequence of zfBLP1 shares 50% identity to human Bcl-XL, and contains all four conserved BH domains of the Bcl-2 family proteins. Primary sequence analysis identified a consensus ER retention signal at the C-terminal end of zfBLP1. Northern blot analysis indicated that there were two major and two minor zfBLP1 mRNA species expressed during embryonic development. Among the two major mRNA species, the short one, approx. 3 kb in size, was expressed throughout embryonic development, while the long one, approx. 7 kb long, was not detectable until the gastrula stage. These results suggest that zfBLP1 is a novel Bcl-2 family protein under complicated regulations, and is likely to play an important role in zebrafish oogenesis and embryogenesis.
Attachments:
1. zf-BclXL-BBA2001.pdf
50. Chen, M. H. C., Lin, G. H., Gong, H. Y., Weng, C. F., Chang, C. Y. and Wu, J. L., "The characterization of prepro-insulin-like growth factor-1 Ea-2 expression and insulin-like growth factor-1 genes (devoid 81 bp) in the zebrafish (Danio rerio).," Gene. 268(1-2):67-75., (IF= 2.082; GENETICS & HEREDITY, 106/164), May, 2001.
In this study, we cloned zebrafish (Danio rerio) IGF-1 cDNA and gene from zebrafish brain cDNA library and adult zebrafish genomic library, respectively. Based on two cDNAs sequence with different length of 5'- and 3'-untranslated region (5UTR and 3UTR) and one nucleotide difference at glutamine (A9, CAG) of A domain represented at IGF-1 sequence. One of zebrafish IGF-1 genes named as IGF-1a gene. The zebrafish IGF-1a gene spanned approximately 15 kb and is divided into five exons. The results of IGF-1 cDNA and genomic Southern blotting, all indicated that the zebrafish have more than one IGF-1 gene. The genomic organization of zebrafish IGF-1a gene in an exon is devoid of 81 bp segment which is located at 3' end of exon 3 encoded 27 amino acid of E domain. The segment of 27 amino acid exists in known teleost IGF-1 genes but is absent in zebrafish IGF-1 gene. The E domain of zebrafish IGF-1 Ea-2 is encoded by 3' end of exon 3 (16 amino acid), full of exon 4 (12 amino acid) and exon 5 (19 amino acid). The sequence data revealed the zebrafish IGF-1a gene encoded IGF-1a Ea-2 mRNA. In combination RT-PCR with Southern blotting, zebrafish IGF-1 genes abundantly expressed IGF-1 Ea-2 mRNA in all tested adult tissues and developmental stages of embryo. The IGF-1 Ea-2 mRNA was first detected during embryo development from blastula stage to hatching, during yolk absorption and at feeding. All these findings suggest that the expression of pro-IGF-1 Ea-2 is not controlled by alternative splicing but alternative gene usage in the zebrafish.
51. Chen MC, Gong HY, Cheng CY, Wang JP, Hong JR, Wu JL., "Cloning and characterization of a novel nuclear Bcl-2 family protein, zfMcl-1a, in zebrafish embryo.," Biochem Biophys Res Commun. 279(2):725-31., (IF= 2.282; BIOCHEMISTRY & MOLECULAR BIOLOGY, 191/291), Dec, 2000.
The importance of the Bcl-2 family proteins in normal vertebrate embryogenesis is being recognized; however, their regulatory mechanism is poorly understood. To elucidate the embryonic roles of Bcl-2 family proteins, we cloned and characterized the first zebrafish Bcl-2 family protein, zfMcl-1a. Zebrafish Mcl-1a shows the highest homology to rat Mcl-1 and contains several conserved BH domains of the Bcl-2 family proteins. It also contains a nuclear localization signal (NLS). Using EGFP reporter analysis, we verified the nuclear localization of zfMcl-1a. Deletion of the NLS resulted in distribution of the fusion protein in the cytoplasm. Northern blot analysis indicated that zfMcl-1a mRNA is 1.5 kb and was expressed in oocytes and throughout embryonic development. Notably, the expression of zfMcl-1a transcript was significantly downregulated during gastrulation. These results suggest that zfMcl-1a is a novel nuclear Bcl-2 family protein and is likely to play an important role in zebrafish oogenesis and embryogenesis.
Attachments:
1. zfMCL1a-BBRC2000.pdf
52. Weng, C. F., Gong, H. Y., Wu, J. L., Hew, C. L. and Hwang, P. P., "Antagonizing effects of type I antifreeze protein on Ca++ uptake in fish TO2 cells.," Zoological Studies. 39 (2):144-150., (IF= 1.014; ZOOLOGY, 76/152), Feb, 2000.
The antifreeze proteins (AFPs) or glycoproteins in several species of polar fish, which prevent the fish from being frozen in icy seawater, have been suggested for use as cryoprotective agents. The present study examines the effect of AFPs on Ca++ flux in a fish TO-2 cell line. The results indicate that type I AFP inhibits the uptake of Ca++ in a dose-dependent manner, but it does not antagonize the release of Ca++. By contrast, type III AFP shows no effect on Ca++ flux. The addition of calcium antagonists, La+++ (LaCl3), Co++ (CoCl2), or verapamil (L-type Ca++ channel blocker), to the uptake medium did not further enhance the blocking effect of AFP on Ca++ uptake, indicating that type I AFP is a non-selective Ca++ blocker.
53. Chen, M. H. C., Lin, G. H., Gong, H. Y., Lee, C. Y., Chang, C. Y., Chen, T. T. and Wu, J. L., "Cloning and characterization of insulin-like growth factor I cDNA from black seabream (Acanthopagrus schlegeli).," Zoological Studies. 37(3): 213-221., (IF= 1.014; ZOOLOGY, 76/152), Apr, 1998.
Cloning and characterization of insulin-like growth factor I cDNA from black seabream (Acanthopagrus schlegeli ). Zoological Studies 37(3): 213-221. A cDNA library was constructed in Uni-ZAP XR using mRNA from the liver of black seabream, Acanthopagrus schlegeli. In this study, we designed a pair of primers from the C and E domains of trout IGF-I cDNA, and synthesized an internal probe of IGF-I from the liver of black seabream by reverse transcription/polymerase chain reaction (RT/PCR). Using the internal probe to screen the cDNA library, we obtained 16 positive clones. Subsequent restriction enzyme map analysis suggested that about 6 forms ranging in size from 1.8 kilo-base pair (kb) to 2.3 kb of IGF-I were present. We took 2 longer cDNA clones to read the full sequence. Sequences of 2 clones of IGF-I cDNAs were found to be 2238 base pairs (bp) and 2299 bp in length. Except for a sequence of 61 nucleotides missing in the 5'-untranslated region (5'-UTR) and 6 nucleotides being different in the 3'-untranslated region (3'-UTR), the other nucleotides of these 2 clones are identical. This showed that the black seabream IGF-I gene contains polymorphism or gene duplication. Both cDNAs containing an open reading frame (ORF) encode 185 amino acids, including a 44-amino acids leader peptide, the 67-amino acids mature peptide in the B, C, A, and D domains, and a 74-amino acids extended carboxyl-terminal peptide in the E domain. The predicted propeptide of IGF-I can be divided into B, C, A, D, and E domains. Owing to the conserved mature peptide of IGF-I, we compared IGF-I of other teleosts with that of black seabream. The comparisons showed 100%, 91%, 91%, 87%, 87%, 58%, and 48% amino acid identity, respectively, with the IGF-I of Sparus, salmon, trout, carp, catfish, hagfish, and amphioxus. Moreover, we compared the E domain of IGF-I of Salmonidae with that of black seabream. The comparisons revealed that the E domain of black seabream belongs to the largest Ea form. These data imply that black seabream cDNAs encode a particular subtype of IGF-I from liver, IGF-I Ea-4. Furthermore, this IGF-I is phylogenetically most closely related to that of Sparidae.
International Paper
1. Wai-Kwan Chu#, Shih-Chin Huang, Yu-Ho Lin, Jen-Leih Wu, Ching-Fong Chang, Hong-Yi Gong*, "Targeted mutagenesis of dead end (dnd1) gene for infertility control of zebrafish and angelfish by CRISPR-Cas9 genome editing.," Marine Biotechnology Conference 2019, Joint Conference of the 12th International Marine Biotechnology Conference and the 12th Asia Pacific Marine Biotechnology Conference. Sep.9~Sep.13, 2019. Shizuoka, Japan., (#MBC2019 Student Poster Award), Sep, 2019.
2. Ken Chuian-Fu, Chia-Yu Lin, Yi-Shun Lee, Hong-Yi Gong, Jen-Leih Wu., "Increasing Omega-3 Polyunsaturated Fatty Acids in Tilapia Can Upregulate nlrc3 and Reduce Streptococcus agalactiae Induced Inflammation by Transcriptomic and Metagenomic Analysis.," Marine Biotechnology Conference 2019, Joint Conference of the 12th International Marine Biotechnology Conference and the 12th Asia Pacific Marine Biotechnology Conference. Sep.9~Sep.13, 2019. Shizuoka, Japan., Sep, 2019.
3. Hong-Yi Gong*, Sheng-Han Wu, Hsin-Yiu Chou, Jen-Leih Wu, "Granulin peptide GRN-41 of Mozambique tilapia is a novel antimicrobial peptide against Vibrio species," Marine Biotechnology Conference 2019, Joint Conference of the 12th International Marine Biotechnology Conference and the 12th Asia Pacific Marine Biotechnology Conference. Sep.9~Sep.13, 2019. Shizuoka, Japan., Sep, 2019.
4. Chang-Wen Huang*, Jia-Xian Li, Yun-Kai Hsueh, Te-Hua Hsu, Hong-Yi Gong, "Cold-Tolerance SSR and SNP Markers Based on Transcriptomic Profiles in Taiwan Tilapia Strains.," Marine Biotechnology Conference 2019, Joint Conference of the 12th International Marine Biotechnology Conference and the 12th Asia Pacific Marine Biotechnology Conference. Sep.9~Sep.13, 2019. Shizuoka, Japan., Sep, 2019.
5. Jen-Leih Wu*, Shin-jie Huang, Hong-Yi Gong, Hui-chin Huang., "Changes in intestinal microbiota composition and cold tolerance in omega-3 enriched tilapia.," Marine Biotechnology Conference 2019, Joint Conference of the 12th International Marine Biotechnology Conference and the 12th Asia Pacific Marine Biotechnology Conference. Sep.9~Sep.13, 2019. Shizuoka, Japan., Sep, 201, Sep, 2019.
6. Hong-Yi Gong, "Mozambique tilapia granulin GRN-41 is a novel host defense peptide against Vibrio pathogens," 2019 International Orchid Conference. Strengthening of a new French-Taiwanese interdisciplinary research network: Innovation in the studies of the natural and artificial aquatic systems, July 9th-11th, Lille & Boulogne sur Mer, France. (Invited speaker), Jul, 2019.
7. Chu WK, Huang SC, Lin YH, Wu JL, Chang CF, Gong HY*., "Targeted mutagenesis of dead end (dnd1) gene for infertility control of zebrafish and angelfish by CRISPR-Cas9 genome editing," 2019 International Orchid Conference. Strengthening of a new French-Taiwanese interdisciplinary research network: Innovation in the studies of the natural and artificial aquatic systems, July 9th-11th, Lille & Boulogne sur Mer, France. (poster), Jul, 2019.
8. Pan YJ, Broux O, Gong HY, Depose E, Rayappa N, Bialais C, Souisssi A, Sadovskaya I, Hwang JS, Souissi S., "The study of copepod aquaculture: progress, challenges and future perspectives using biotechnology.," 2019 International Orchid Conference. Strengthening of a new French-Taiwanese interdisciplinary research network: Innovation in the studies of the natural and artificial aquatic systems, July 9th-11th, Lille & Boulogne sur Mer, France., Jul, 2019.
9. Gong HY*, Ho CH, Wu SH, Lin HJ, Lin HY., "Herbmedotcin as an antibiotic substitute applied in the tilapia aquaculture," 12th Asia Fisheries and Aquaculture Forum. Transforming Asia Fisheries and Aquaculture for Sustainable Production and Nutrition. 8-12, April, 2019. Iloilo city, Philippines., (invited speaker), Apr, 2019.
Special Section 6: Application of Hernmedotcin, a high efficient and safe alternative of antibiotics in aquaculture.
10. Gong, H. Y.*, Tseng, P. C., Tai, T. Y., Lin, F. Y., Yeh, S. L., Wu, J. L., Chou, H. Y., Huang, C. W., "A deletion and microsatellite DNA markers in myocyte enhancer factor 2d (mef2d) gene are associated with the growth trait of giant grouper.," The 4th NTU-NTOU Joint International Vietnam-Taiwan Conference on Advanced Marine Aquaculture., Dec.11-12, 2018. Nha Trang University, Nha Trang, Vietnam., Dec, 2018.
11. Sheng-Han Wu and Hong-Yi Gong*, "Mozambique tilapia granulin GRN-41 is a novel host defense peptide against marine Vibrio pathogens," 2018 International Symposium of Agricultural Biotechnology. Sep. 21, 2018. National Pingtung University of Science and Technology, Taiwan., (*Invited Speaker), Sep, 2018.
12. Gong, H. Y.*, Pham, H. N., Ho, C. H., Tseng, P. C., Wu, S. H., Kuo, Y. H., Huang, C. W., "Development of hepcidin family genes associated microsatellites for marker-assisted selection of disease-resistant tilapia.," Asia-Pacific Aquaculture 2018 (APA 18), Taipei, Taiwan. April 23-26, 2018. World Aquaculture Society., Apr, 2018.
13. Chia-Hui Ho, Sheng-Han Wu, Hong-Yi Gong*, "Nile tilapia hepatic antimicrobial peptide Hamp genes encoding four hepcidin peptides were differentially activated in response to Sreptococcus iniae infection.," Asia-Pacific Aquaculture 2018 (APA 18), Taipei, Taiwan. April 23-26, 2018. World Aquaculture Society., Apr, 2018.
14. Sheng-Han Wu, Hong-Jie Lin, Hong-Yi Gong*, "Tilapia GRN-41 Peptide Derived from Short-form Progranulin PGRN1 Gene Exerts Immune Modulation and Antibacterial Activities.," Asia-Pacific Aquaculture 2018 (APA 18), Taipei, Taiwan. April 23-26, 2018. World Aquaculture Society., Apr, 2018.
15. Jia-xian Li, Pei-Yun Chu, Ling Hsu, Hong-Yi Gong, Chang-Wen Huang*, "COLD TOLERANCE GENE-ASSOCIATED MARKERS DISCOVERY FROM THE TRANSCRIPTOME DATABASE BY NEXT GENERATION SEQUENCING IN NILE TILAPIA Oreochromis niloticus," Asia-Pacific Aquaculture 2018 (APA 18), Taipei, Taiwan. April 23-26, 2018. World Aquaculture Society., Apr, 2018.
16. Po-Chun Tseng, Tse-Yu Tai, Feng-You Lin, Shinn-Lih Yeh, Chang-Wen Huang, Hong-Yi Gong*, "A DELETION AND MICROSATELLITE DNA MARKERS IN THE 3’-UTR OF MEF2D GENE ARE ASSOCIATED WITH GROWTH OF GIANT GROUPER Epinephelus lanceolatus," Asia-Pacific Aquaculture 2018 (APA 18), Taipei, Taiwan. April 23-26, 2018. World Aquaculture Society., Apr, 2018.
17. Yi-Ting Tsai, Sheng-Han Wu and Hong-Yi Gong*, "Myostatin a and b double knockout causes not only myocyte hyperplasia but also apoptosis and mortality in adult zebrafish.," The JSFS 85th Anniversary-Commemorative International Symposium “Fisheries Science for Future Generations”. Sep.22-24, 2017. Tokyo University of Marine Science and Technology, Shinagawa, Tokyo, Japan., Sep, 2017.
18. Chia-Hui Ho, Sheng-Han Wu and Hong-Yi Gong*., "Novel hepatic antimicrobial peptide HAMP3 genes are strongly activated not only in the liver but also in gill, head kidney and spleen of Nile tilapia to defend against virulent Streptococcus iniae.," The JSFS 85th Anniversary-Commemorative International Symposium “Fisheries Science for Future Generations”. Sep.22-24, 2017. Tokyo University of MArine Science and Technology, Shinagawa, Tokyo, Japan., Sep, 2017.
19. Hong Nhat Pham, Chia-Hui Ho, Po-Chun Tseng, Yi-Hsuan Kuo, Sheng-Han Wu, Chang-Wen Huang and Hong-Yi Gong*., "Highly amplification of hepcidin genes and associated microsatellites are potential molecular markers in marker-assisted selection for disease resistance of Nile tilapia.," The JSFS 85th Anniversary-Commemorative International Symposium “Fisheries Science for Future Generations”. Sep.22-24, 2017. Tokyo University of MArine Science and Technology, Shinagawa, Tokyo, Japan., Sep, 2017.
20. Hong-Yi Gong#*, Chia-Hui Ho, Sheng- Han Wu, Hong Nhat Pham, Po-Chun Tseng, Yi-Hsuan Kuo, Chang-Wen Huang, and Jen-Leih Wu, "Antimicrobial peptide hepcidin genes were highly amplified in the genome of Nile tilapia.," 11th Asia-Pacific Marine Biotechnology Conference (APMBC 2017), May 22-24, 2017, University of Hawaii at Manoa, Hawaii, USA., (#oral presentation), May, 2017.
21. Chang-Wen Huang#*, Hong-Yi Gong*, Hsin-Yiu Chou, Shinn-Lih Yeh, and Kun-Tsai Tai., "Assessment of the genetic diversity in the broodstock and development of the growth-related markers from the transcriptome for marker-assisted selection in giant grouper, Epinephelus lanceolatus.," 11th Asia-Pacific Marine Biotechnology Conference (APMBC 2017), May 22-24, 2017, University of Hawaii at Manoa, Hawaii, USA., (#oral presentation), May, 2017.
22. Yi-Ting Tsai#, Sheng-Han Wu, and Hong-Yi Gong*, "Double knockout of myostatin a and b genes causes muscle enhancement in zebrafish by CRISPR/Cas9 technology?," 11th Asia-Pacific Marine Biotechnology Conference (APMBC 2017), May 22-24, 2017, University of Hawaii at Manoa, Hawaii, USA., (#oral presentation), May, 2017.
23. Shin-Jie Huang#, Ching-Yu Huang, Hong-Yi Gong, Jyh-Yih Chen, Chuian-Fu Ken, and Jen-Leih Wu*., "Improvement of omega-3 polyunsaturated fatty acid biosynthesis and cold tolerance in zebrafish and tilapia by transgenesis.," 11th Asia-Pacific Marine Biotechnology Conference (APMBC 2017), May 22-24, 2017, University of Hawaii at Manoa, Hawaii, USA, May, 2017.
24. Gong HY*, Huang SC, Wei ZZ, Tseng PC, Wu SH, Wu JL., "Development of novel transgenic pink angelfish and transgenic tilapia as a bioreactor by zebrafish muscle-specific promoter/enhancer," International Marine Biotechnology Conference 11th Triennial Conference IMBC 2016. 29 August- 2 September. Baltimore, Maryland, USA., (oral presentation), Aug, 2016.
25. Huang SJ, Huang CY, Cheng CL, Gong HY, Wu JL., "Metabolic engineering of omega-3 polyunsaturated fatty acid synthesis enzymes increase tolerance from cold stress in zebrafish.," International Marine Biotechnology Conference 11th Triennial Conference IMBC 2016. 29 August- 2 September. Baltimore, Maryland, USA., Aug, 2016.
26. Chu PY, Chan WR, Liou CH, Huang YS, Gong HY, Wang YH and Huang CW., "De novo sequencing and assembly of transcriptome in the ornamental shrimp (Neocaridina denticulate var.)," International Marine Biotechnology Conference 11th Triennial Conference IMBC 2016. 29 August- 2 September. Baltimore, Maryland, USA., Aug, 2016.
27. Gong HY*, Tseng PC, Wei ZZ, Huang SC, Chin HY, Wu SH, Ho CH, Lin WF, Lin CC, Huang MC, Chou CH, Wu JL., "Establishment of fast-growing transgenic tilapia as a bioreactor for the production of fish scale-derived artificial corneas," 2016 Pacific Science Congress. Science, Technology and Innovation. 13-17 June, 2016. Academia Sinica, Taipei, Taiwan., Jun, 2016.
28. Gong HY*, Ho CH, Wu SH, Lin WF, Kuo YH, Chang YH., "Three HAMP genes of Nile tilapia were differentially activated in spleen to defend against Streptococcus iniae infection.," 2nd International Conference of Fish & Shellfish Immunology. Portland, Maine, USA. June 26-30., Jun, 2016.
29. Hong-Yi Gong*, Clare Siing-Nga Tang, Sheng-Han Wu, Wen-Fu Lin, Hui-Yen Chin, "A Novel Tilapia Short Progranulin Gene PGRN2 Modulates Innate Immunity in Transgenic Zebrafish Model," International Society of Developmental and Comparative Immunology (ISDCI) Congress 2015, Spain Murcia, June 29- July 3, 2015., Jul, 2015.
30. Sheng-Han Wu, Hong-Jie Lin, Wen-Fu Lin and Hong-Yi Gong*, "Tilapia granulin peptide GRN-41 contributes to activate innate immune response against Vibrio vulnificus infection in transgenic zebrafish," International Society of Developmental and Comparative Immunology (ISDCI) Congress 2015, Spain Murcia, June 29- July 3, 2015., Jul, 2015.
31. Hong-Yi Gong*, "Short Progranulin Genes Amplified in Tilapia Genome Were Involved in Immune Modulation and Pathogen Resistance," 10th Asia-Pacific Marine Biotechnology Conference (2014 APMBC) May 4- May 7, 2014. Academia Sinica, Taipei, Taiwan., S1- Biotechnology and Sustainable Aquaculture. S1-6, p27-28. (Invited Speaker), May, 2014.
32. Sheng-Han Wu, Hong-Jie Lin, Clare Siing Nga Tang, Chia-Wen Lu, Hong-Yi Gong*, "Tilapia short form progranulin genes contribute to immune defense against bacterial infection.," 10th Asia-Pacific Marine Biotechnology Conference (2014 APMBC), May 4- May 7, 2014. Academia Sinica, Taipei, Taiwan., May, 2014.
33. Chang CY, Sung YC, Gong HY, Huang CW, Wu JL*., "MicroRNA-133 modulates skeletal muscle hypertrophy through binding JunB transcription factor mRNA in zebrafish," 10th Asia-Pacific Marine Biotechnology Conference (2014 APMBC), May 4- May 7, 2014. Academia Sinica, Taipei, Taiwan., May, 2014.
34. Huang CW, Chi JR, Gong HY, Chen RH, Chang SJ, Liu FG, Wu JL*., "Skeletal muscle growth-related miRNAs expression signature and application of marker-assisted selection in Nile tilapia," 10th Asia-Pacific Marine Biotechnology Conference (2014 APMBC), May 4- May 7, 2014. Academia Sinica, Taipei, Taiwan., May, 2014.
35. Gong H.Y.*, Chin H.Y., Tai T.Y., Tseng P.C., Huang S.C., "Muscle-specific expression of giant grouper growth hormone in transgenic zebrafish leads to muscle hypertrophy and hyperplasia," The 7th Asia and Oceania Society for Comparative Endocrinology (AOSCE) intercongress, March 18-24. NTOU, Keelung, Taiwan, Mar, 2014.
36. Hong-Yi Gong*, Tse-Yu Tai, Fcng-You Lin, Hsin-Yiu Chou, Chang-Wen Huang, Shinn-Lih Yeh and Jen-Leih Wu, "Development of Type I microsatellite markers from transcriptome of giant grouper for marker-assisted selection," International Marine Biotechnology Conference, Brisbane, Australia 2013 (IMBC 2013). Nov. 11-15, 2013., Nov, 2013.
37. Liu W, Chen YC, Lin CH, Huang SJ, Gong HY, Wu JL., "The role of foxm1 in the initiation mechanism of intrahepatic cholangiocarcinogenesis in zebrafish," International Marine Biotechnology Conference, Brisbane, Australia 2013 (IMBC 2013). Nov. 11-15, 2013., Nov, 2013.
38. Hong-Yi Gong*, Shih-Chin Huang, Sheng-Han Wu, Clare Siing-Nga Tang, Hong-Jie Lin, Hui-Yen Chin, "Novel Teleost Muscle-Specific Enhancer/Promoter Applied in Transgenic Fluorescent Angelfish and Study of Tilapia Innate Immunity," The 5th Joint Forum of Environmental Sciences. Nov.18-19. NTOU, Keelung, Taiwan., Taiwan, Japan, Korea Joint Forum (oral), Nov, 2013.
39. Hong-Yi Gong*, Sheng-Han Wu, Hui-Yen Chin, Hong-Jie Lin, Clare Siing-Nga Tang, Yen-Hsing Li, Jen-Leih Wu., "Mozambique tilapia short progranulin genes contribute to defense against bacterial pathogens.," Special section on the First International Conference of Fish and Shellfish Immunology, June 25-28, 2013 in Vigo, Spain. Fish and Shellfish Immunology, 34(6): 1653., Jun, 2013.
40. Hong-Yi Gong*, Sheng-Han Wu, Hui-Yen Chin, Hong-Jie Lin, Yen-Hsing Li, Mark Hung-Chih Chen, Shao-Yang Hu, Chang-Wen Huang, Jen-Leih Wu, "Novel Tilapia Granulin Peptide GRN-41 Is An Innate Immune Modulator.," The 9th Asia-Pacific Marine Biotechnology Conference (APMBC), July13-16, 2012. Kochi, Japan, Jul, 2012.
41. Chih-Chi Chen, Yen-Hsing Li, Jen-Leih Wu, Koichi Kawakami, Hong-Yi Gong*, "Antagonistic roles of Akirin1 and Akirin2 in regulating muscle growth of zebrafish.," The 10th International Conference on Zebrafish Development and Genetics, June 20-24, 2012. University of Wisconsin-Madison, USA., Jun, 2012.
42. Yen-Hsing Li, Hsu-Yu Chen, Ya-Wen Li, Sung-Yu Wu, Gen-Hwa Lin, Shao-Yang Hu, Chia-Hsuan Liao, Hong-Yi Gong, Chang-Wen Huang and Jen-Leih Wu., "Progranulin is required for the maintenance of myogenic progenitors cells in postembryonic myogenesis in zebrafish.," The 10th International Conference on Zebrafish Development and Genetics. June 20-24, 2012. University of Wisconsin-Madison, USA., Jun, 2012.
43. Gong, Hong-Yi, "Development of Polymorphic Microsatellite DNA Markers from the Transcriptome of Giant Grouper for Marker-Assisted Selection.," Vietnam-Taiwan Symposium On Seed Breeding Technology And Marine Aquaculture. May 25th-26th, 2012, Nha Trang University, Nha Trang, Vietnam, (invited speaker), May, 2012.
44. Chin, H. Y., Wu, S. H., Lin, H. J., Tang, C. S. N., Wu, J. L. and Gong, H. Y.*, "Tilapia short PGRN genes contribute to defense against bacterial pathogens.," 2012 Taiwan – Japan Symposium on Emerging Trends in Aquatic Animal Immunity and Aquaculture Biotechnology. Feb. 21~Feb. 22. National Cheng Kung University, Tainan, Taiwan., Feb, 2012.
45. Gong, H. Y.*, Chin, H. Y., Tai, T. Y., Wu, S. H., Nan, F. H., Huang, C. W., Wu, J. L., Hong, J. R., and Yeh, S. L., "Establishment of transcriptome to develop functional genes and microsatellites as type I markers of giant grouper Epinephelus lanceolatus.," International Symposium on Grouper Culture. Nov. 8-11, 2011, Pingtung, Taiwan. P-14. (oral report), Nov, 2011.
46. Chen, C. C., Lin, H. J., Liu, W., Wu, J. L., Kawakami, K., Gong, H. Y.*, "Transgenic zebrafish established by muscle-specific TetOff system to study functional genes and as potential tetracycline biosensor.," The 4th Joint Forum of Environmental Sciences 2011. 20th ~21th June, 2011. Ehime University, Japan. (oral report), Jun, 2011.
47. Chen, C. C., Hu, M. C., Wu, J. L., Li, Y. H., Hu, S. Y., Kawakami, K., Gong, H. Y.*, "Muscle-specific overexpression of Akirin1 activates myogenic regulatory factors and IGF2b that leads to muscle hypertrophy in transgenic zebrafish.," 44th Annual Meeting for the Japanese Society of Developmental Biologists Jointly Sponsored by the Asia-Pacific Developmental Biology Network. Okinawa, May 19-21, 2011. P-1157., May, 2011.
48. Li, Y. H., Chen, H. C., Gong, H. Y., Hu, S. Y., Li, Y. W., Lin, G. H., Lin, C. C., Liu, W., Wu, J. L.*, "Progranulin A-mediated MET Signaling Is Essential for Liver Morphogenesis in Zebrafish.," 44th Annual Meeting for the Japanese Society of Developmental Biologists Jointly Sponsored by the Asia-Pacific Developmental Biology Network. Okinawa, May 19-21, 2011. P-2101., May, 2011.
49. Gong, H. Y.*, Lin, H. J., Wu, S. H., Hu, M. C., Nan, F. H., Sheen, S. S., Chen, M. H., Li, Y. H., Huang, C. W., "Muscle-specific overexpression of novel granulin peptides from tilapia progranulin gene family activates innate immunity genes and myogenic regulatory factors in transgneic zebrafish.," The 9th Asian Fisheries and Aquaculture Forum. April 21-25, 2011. Shanghai, China. S5-24. (oral report), Apr, 2011.
50. Gong, H. Y.*, Chen, M. C., Huang, S. C., Hu, M. C., Lin, C. H., Hu, S. Y., Fan, T. Y., Kawakami, K. and Wu, J. L., "Establishment of transgenic zebrafish expressing Taiwan Acropora coral fluorescent proteins by a novel zebrafish muscle-specific promoter/enhancer.," The 9th International Marine Biotechnology Conference. IMBC2010. Qingdao, China, October 8-12, 2010., Oct, 2010.
51. Hu, S. Y., Lin, P. Y., Liao, C. H., Gong, H. Y., and Wu, J. L.*, "Technology development for infertile control of genetically modified zebrafish.," The 9th International Marine Biotechnology Conference. IMBC2010. Qingdao, China, October 8-12, 2010., Oct, 2010.
52. Li, Y. H., Chen, M. H. C., Hu, S. Y., Li, Y. W., Lin, G. H., Lin, C. C., Gong, H. Y., Liu, W. and Wu, J. L.*, "Progranulin, a novel regulator of Met, is required for embryonic hepatic outgrowth.," 43rd Annual Meeting for the Japanese Society of Developmental Biologists Jointly Sponsored by the Asia-Pacific Developmental Biology Network. Kyoto, June 20-23, 2010. P-065., Jun, 2010.
53. Shao-Yang Hu, Hong-Yi Gong, Jim-Ray Chen, Gen-Hwa Lin, Jen-Leih Wu, "MicroRNA-206 regulates sonic hedgehog to control myogenesis," Mechanisms of Development 126, S58, Aug, 2009.
54. Hu, M. C., Wu, J. L. and Gong, H. Y.*, "Roles of spliced XBP-1, a key regulator of unfolded protein response (UPR) induced by ER stress in zebrafish.," The 2nd Joint Forum of Environmental Sciences 2009. 28th ~29th July, 2009. Chonnam National University, Yeosu, Korea. (oral report)), Jul, 2009.
55. Gong, H. Y., Lin, M. Y., Huang, H. P., Hu, S. Y., Lin, G. H., Liu, W., Hu, M. C., Kawakami, K., Wu, J. L., "Overepression of Zebrafish IMP2 Induces Steatosis and ER Stress in the Liver of Transgenic Zebrafish as an Animal Model of Human Non-Alcoholic Fatty Liver Disease.," 8th International Meeting on Zebrafish Development and Genetics. June25~June29, 2008. The University of Wisconsin-Madison, USA., Jun, 2008.
56. Hu, S. Y., Gong, H. Y., Kawakami, K., Lin, G. H., Wu, J. L., "Development of Platform Technology for Sterile Control of Genetically Modified Fish.," 8th International Meeting on Zebrafish Development and Genetics. June25~June29, 2008. The University of Wisconsin-Madison, USA, Jun, 2008.
57. Gong, H. Y., Hu, S. Y., Liu, W., Lin, W. Z., Chang, Z. K., Kawakami, K., Shih, C., Wu, J. L.*, "Chimeric and Virus-derived Liver-Specific Promoters in Transgenic Zebrafish.," International Conference of Advanced Research on Marine Bioresources. May 8-9, 2008 in National Taiwan Ocean University, Keelung, Taiwan., May, 2008.
58. Wang, W. L., Lin, G. H., Gong, H. Y., Hong, J. R., Lu, M. W., Wu, J. L., "Transcription regulation of apoptotic genes in the pathogenesis of infectious pancreatic necrosis virus.," 16th Euroconference on Apoptosis and 5th Swiss Apoptosis Meeting, p179. Switzerland., Jan, 2008.
59. Hu, S. Y., Gong, H. Y., Chen, J. R., Cheng, H. W., Wang, W. P., Lin, C.J.F., Amaili, A. A., Wang, W. L., Lin, G. H., Li, Y. H., Chen, M. H. C. and Wu, J. L., "The necessity of sonic hedgehog (Shh) regulation for myogenesis is targeted by miR-1 and miR-206.," The 5th European Zebrafish Genetics and Development Meeting. 12-15 July 2007 in Amsterdam, the Netherlands., Jul, 2007.
60. Gong, H. Y., Lin, C. J. F., Chen, M. H. C., Hu, M. C., Lin, G. H., Wu, J. L., "Dominant-negative mutants in NLS of a novel zebrafish hepatocyte nuclear factor 1 repress transactivation activity of wild-type HNF1gamma for zebrafish IGF-1 promoter.," International Marine Biotechnology Conference. IMBC2005. St. John's, Newfoundland & Labrador, Canada, June 7-12, 2005, PS.C07.166.rado, Jun, 2005.
61. Chen, M. H. C., Chang, Y, Li, Y. H., Lin, G. H., Gong, H. Y., Wu, J.L., "Growth genes identification through growth-related hormone stimulation.," International Marine Biotechnology Conference. IMBC2005. St. John's, Newfoundland & Labrador, Canada, June 7-12, 2005, C09-1.007., Jun, 2005.
62. Lin, G. H., Chen, M. H. C., Gong, H. Y., Hsieh, D. J. Y., Lin, C. J. F., Wu, J. L., "Functional analysis of insulin-like growth factor 1 receptor gene promoter in the zebrafish.," Marine Biotechnology 6: S146-S152., (IF= 3.43), Jan, 2004.
63. Chen, M.H.C., Gong, H.Y., Hsieh, D.J.Y., Lin, C.J.F., Lin, G.H., Wu, J.L., "The insulin-like growth factor 2 involve in thorax development in zebrafish (Danio rerio).," Marine Biotechnology Conference 2003, p28, Japan., Jan, 2003.
64. Gong, H. Y., Chen, M. H. C., Hu, M. C., Lin, G. H., Wu, J. L., "Unfolded protein response is conserved in the generation of activated ATF6 and spliced form XBP1 transcription factors in teleosts.," Marine Biotechnology Conference 2003, p197, Japan., Jan, 2003.
65. Lin, G. H., Chen, M. H. C., Gong, H. Y., Hsieh, D. J. Y., Lin, C. J. F., Wu, J. L., "Regulation of insulin-like growth factor 1 receptor gene promoter in the zebrafish.," Marine Biotechnology Conference 2003, p148, Japan., Jan, 2003.
66. Gong, H.Y., Chen, M.H.C., Hu, M.C., Hong, J.R., Lin, G.H. and Wu, J.L., "Functional differences of two zebrafish XBP1 proteins encoded by single xbp1 gene in zebrafish embryonic development.," The 5th International Conference on Zebrafish Development and Genetices. June 12-16, University of Wisconsin-Madison, USA., Jun, 2002.
67. Chen, M.H.C., Gong, H.Y., Hsieh, J.Y., Lin, G.H., Huang, W.T., Weng, C.F. and Wu, J.L., "Roles for IGF system during post-fertilization development: in vivo real-time imagination of IGF in the system biology.," The 5th International Conference on Zebrafish Development and Genetices. June 12-16, University of Wisconsin-Madison, USA., Jun, 2002.
68. Hong, J.-R., Gong, H. Y. and Wu, J.-L., "IPNV VP5, a novel anti-apoptosis of Bcl-2 family, regulate Mcl-1 and viral protein expression.," The 2001 meeting on Program Cell Death. Nov. 8-Nov.13, 2001. Cold Spring Harbor Laboratory. USA. pp106., Nov, 2001.
69. Wang, J.P., Chen, M.C., Gong, H.Y., Hong, J.R. and Wu, J.L., "Cloning and characterization of zfBax1, a pre-apoptotic Bcl-2 family protein, in zebrafish embryos.," The 2001 meeting on Program Cell Death. Nov. 8-Nov.13, 2001. Cold Spring Harbor Laboratory. USA. pp262., Nov, 2001.
70. Gong, H.Y., Hu, M.C., Chen, M.H.C., Lin, C.J.F., Lin, G.H and Wu, J.L., "Roles of hepatocyte nuclear factor 1 (HNF1) family from zebrafish (Danio rerio) in embryonic development and IGF1 gene regulation.," Postgraduate Conference on Marine Biology and Biotechnology. The Chinese University of Hong Kong, Shatin, Hong Kong. pp49. (Invited), Jun, 2001.
71. Gong, H.Y., Chen, M.H.C., Lin, C.J.F., Lin, G.H., Huang, W.T., Weng, C.F., and Wu, J.L., "Two distinct insulin-like growth factor 2 (IGF2) precursors from zebrafish (Danio rerio) express in embryonic development.," International Marine Biotechnology Symposium (IMBS) 2001. Heading into post-genome era., Jan, 2001.
72. Huang, W.T., Lin, C.J.F., Gong, H.Y., Chen, M.H.C., Weng, C.F. and Wu, J.L., "Hepatocyte nuclear factors (HNFs) –1alpha, -1beta and –3gamma express in the gonad of tilapia (Oreochromis mossambicus).," The 9th Society of Chinese Bioscientists in America (SCBA) International Symposium. Frontiers of Biotechnology & Biomedical Sciences in the New Millennium. pp302., Jan, 2001.
73. Gong, H.Y., Hu, M.C., Lin, C.J.F., Chen, M.H.C., Weng, C.F. and Wu, J.L., "Roles of zebrafish hepatocyte nuclear factor 1 beta (HNF1b) and novel HNF1gamma in embryonic development.," The 9th Society of Chinese Bioscientists in America (SCBA) International Symposium. Frontiers of Biotechnology & Biomedical Sciences in the New Millennium. pp304., Jan, 2001.
74. Gong, H.Y., Huang, W.T., Wu, C.J., Lin, C.J.F., Cheng, C.Y., Wu, J.L. and Weng, C.F. (2001)., "Specific muscle type creatine kinase (CK) isoform responds to acute salinity changes in a euryhaline teleost (Oreochromis mossambicus).," The 9th SCBA International Symposium. Frontiers of Biotechnology & Biomedical Sciences in the New Millennium. pp306., Jan, 2001.
75. Hong, J.R., Chen, M.C., Gong, H.Y., Cheng, C.Y., Wang, J.P. and Wu, J.L., "Apoptotic gene function of IPNV genome and zebrafish (Danio rerio) development.," International symposium on " A Step toward the Great Future of Aquatic Genomics". pp46. Nov.10-12. Tokyo. Japan., Nov, 2000.
76. Gong, H.Y., Hu, M.C., Chen, M.H.C., Chen, M.C., Weng, C.F., Lin, G.H., Lin, C.J.F. and Wu, J.L., "Regulation of three zebrafish hepatocyte nuclear factor 1 (HNF1) members on transactivation of zebrafish IGF-I promoter.," The 2000 meeting on Zebrafish Development and Genetics. April 26-April 30, 2000. Cold Spring Harbor Laboratory. USA. pp97., Apr, 2000.
77. Chen, M.H.C., Lin, G.H., Gong, H.Y., Weng, C.F., Chang, C.Y. and Wu,J.L., "The unique Ea-2 expression of pro-IGF-I genes (devoid 81 bp) in zebrafish containing the stimulatory effect on branchial cartilage sulfation.," The 2000 meeting on Zebrafish Development and Genetics. April 26-April 30, 2000. Cold Spring Harbor Laboratory. USA. pp48., Apr, 2000.
Domestic Paper
1. 蔣中平、蘇昱溥、朱珮云、徐德華、龔紘毅、黃章文*, "利用 RNA-seq 開發臺灣鯛耐鹽相關微衛星分子標記與子代驗證," 109 年度臺灣水產學會會員大會暨學術論文發表會。2020 年 5 月 11-16 日。線上論壇。論文編號:BPC-30。, May, 2020.
2. 簡廷璋、戴昆財、龔紘毅、徐德華、黃章文*, "建立黃鰭石斑(Epinephelus flavocaeruleus)轉錄體資料庫與開發成長相關分子標記," 109 年度臺灣水產學會會員大會暨學術論文發表會。2020 年 5 月 11-16 日。線上論壇。論 文編號:BPC-27。, May, 2020.
3. 陳柏安、薛勻凱、李嘉銜、朱珮云、徐德華、龔紘毅、黃章文*, "利用微衛星標記比較商業與野生臺灣鯛品系抗寒能力及成長性能," 109 年度臺灣水產學會會員大會暨學術論文發表會。2020 年 5 月 11-16 日。線上論壇。論文編號:BPC-32。, May, 2020.
4. 李嘉銜、徐德華、龔紘毅、黃章文, "轉錄組分子標記輔助台灣鯛(羅非魚)耐寒品系選育。," 第九屆海峽兩岸“魚類生理與養殖”學術研討會。2020年1月5-8日,中國海南三亞。第7頁。, Jan, 2020.
5. 徐德華、龔紘毅、黃章文。, "台灣文蛤的身世之謎。," 第九屆海峽兩岸“魚類生理與養殖”學術研討會。2020年1月5-8日,中國海南三亞。第32頁, Jan, 2020.
6. 龔紘毅、朱慧君、 張清風, "以CRISPR/Cas9 基因编辑技术建立deadend(dnd1)标靶突变斑马鱼及淡水神仙鱼之不孕控制技术," 2020 第九届海峡两岸“鱼类生理与养殖研讨会”。2020年1月5-8日,中國海南三亞。第7頁。, Jan, 2020.
7. 龔紘毅, "精準育種在水產養殖的發展潛力," The Aqua Power Forum 發現台灣水產養殖新實力。, 臺灣海洋大學國際產學聯盟。高雄國際會議中心。2019/12/16。, Dec, 2019.
8. Gong HY*, Chu WK, Chang CF, Wu JL., "Targeted mutagenesis of dead end (dnd1) for infertility control of zebrafish and freshwater angelfish.," 2019 Taiwan Zebrafish Symposium, Nov. 16, 2019 in KMU, Kaohsiung, Taiwan., Nov, 2019.
9. Hong-Yi Gong, "Potential of Precision Breeding in Aquaculture," 巴林 (Bahrain)水產經營與養殖培訓班。2019/11/21。, Nov, 2019.
10. Lin ST, Ho CH, Huang SJ, Wu JL, Chen JY, Gong HY*., "Liver-specific expression of fat-1 omega-3 fatty acid desaturase in transgenic zebrafish," 2019 臺灣海洋生物技術學會 第四屆第一次會員大會暨水產飼料添加物開發技術與產業整合研討會。2019年6月28日。國立宜蘭大學。, Jun, 2019.
11. Wu SH, Chou HY, Liu PC, Wu JL, Gong HY*., "Granulin peptide GRN-41 of Mozambique tilapia is a novel antimicrobial peptide against Vibrio species.," 2019 臺灣海洋生物技術學會 第四屆第一次會員大會暨水產飼料添加物開發技術與產業整合研討會。2019年6月28日。國立宜蘭大學。, (吳聖韓榮獲壁報論文競賽博士組佳作), Jun, 2019.
12. Chu WK, Chang CF, Gong HY*., "Targeted mutagenesis of dead end (dnd1) gene for infertility conntrol of zebrafish and angelfish by CRISPR-Cas9 genome editing.," 2019 臺灣海洋生物技術學會 第四屆第一次會員大會暨水產飼料添加物開發技術與產業整合研討會。2019年6月28日。國立宜蘭大學。, (朱慧君榮獲壁報論文競賽碩士及大專組第三名), Jun, 2019.
13. Yu JH, Tsai YT, Gong HY*., "Establishment of myostatin b mutated Nile tilapia by CRISPR/Cas9 genome editing to enhance muscle growth in tilapia," 2019 臺灣海洋生物技術學會 第四屆第一次會員大會暨水產飼料添加物開發技術與產業整合研討會。2019年6月28日。國立宜蘭大學。, Jun, 2019.
14. Chiu YT, Chen PA, Tai KT, Gong HY, Huang CW*., "Novel SNP of actc1 and pcn genes and its association with growth traits in potato grouper (Epinephelus tukula)," 2019 臺灣海洋生物技術學會 第四屆第一次會員大會暨水產飼料添加物開發技術與產業整合研討會。2019年6月28日。國立宜蘭大學。, Jun, 2019.
15. Su YP, Hsu TH, Gong HY, Huang CW*., "Comparative transcriptome analysis reveals salinity regulated gene-based SSR markers in saline tolerant tilapia strain," 2019 臺灣海洋生物技術學會 第四屆第一次會員大會暨水產飼料添加物開發技術與產業整合研討會。2019年6月28日。國立宜蘭大學。, Jun, 2019.
16. Hsueh YK, Li JX, Hsu TH, Gong HY, Huang CW*., "Identification of functional genes and SNP alleles related to cold-tolerance fro wild and farmed tilapia strains," 2019 臺灣海洋生物技術學會 第四屆第一次會員大會暨水產飼料添加物開發技術與產業整合研討會。2019年6月28日。國立宜蘭大學。, (薛勻凱榮獲壁報論文競賽碩士及大專組佳作), Jun, 2019.
17. 龔紘毅*、曾柏鈞、黃章文、吳金洌、林峰右、葉信利, "龍膽石斑及其雜交斑之生長分子標誌輔助選育," 2019海洋科技跨校聯合論壇及產學媒合會。2019年5月28日。中研院。, May, 2019.
18. 黃章文、徐德華、龔紘毅。, "分子標誌輔助優質臺灣鯛種苗精準選育技術。," 2019海洋科技跨校聯合論壇及產學媒合會。2019年5月28日。中研院。, May, 2019.
19. 朱慧君、黃士晉、龔紘毅*, "Establishment of infertile angelfish (Pterophyllum scalare) with mutated dead end gene by CRISPR/Cas9 genome editing technology," 2019臺灣水產學會年會會員大會暨學術論文發表會。2019年1月12日。台灣大學。, (榮獲水產生物技術、水產疾病及微生物組佳作), Jan, 2019.
20. 林詩庭、何佳蕙、黃信傑、吳金洌、陳志毅、龔紘毅*, "建立肝臟或腸道專一性表現之 fat-1 omega-3 去飽和酶基因轉殖斑馬魚與吳郭魚以增進其抗病原菌能力之機制研究," 2019臺灣水產學會年會會員大會暨學術論文發表會。2019年1月12日。台灣大學。, Jan, 2019.
21. 游睿軒、蔡宜庭、龔紘毅*, "Establishment of myostatin b mutated Nile tilapia by CRISPR/Cas9 genome editing to enhance muscle growth in tilapia," 2019臺灣水產學會年會會員大會暨學術論文發表會。2019年1月12日。台灣大學。, Jan, 2019.
22. 簡廷璋、戴昆財、龔紘毅、 黃章文*, "黃鰭石斑魚(Epinephelus flavocaeruleus)粒線體 DNA COI 區域定序之 PCR-RFLP 種原鑑定標記開發," 2019臺灣水產學會年會會員大會暨學術論文發表會。2019年1月12日。台灣大學。, Jan, 2019.
23. 薛勻凱、李嘉銜、王姿曼、 徐德華、龔紘毅、黃章文*, "抗寒分子標記輔助臺灣鯛品系加值選育," 2019臺灣水產學會年會會員大會暨學術論文發表會。2019年1月12日。台灣大學。, Jan, 2019.
24. 邱宇廷、陳柏安、戴昆財、 龔紘毅、黃章文*, "金錢斑種群基因多型性與成長連鎖標記開發," 2019臺灣水產學會年會會員大會暨學術論文發表會。2019年1月12日。台灣大學。, Jan, 2019.
25. 蘇昱溥、徐德華、龔紘毅、 黃章文*, "建立吳郭魚耐鹽轉錄體資料庫與開發 DNA 分子標記," 2019臺灣水產學會年會會員大會暨學術論文發表會。2019年1月12日。台灣大學。, Jan, 2019.
26. 徐德華*、潘婕玉、呂明偉、 林正輝、龔紘毅、黃章文, "分子標記輔助六種台灣主要放流魚種的來源判別," 2019臺灣水產學會年會會員大會暨學術論文發表會。2019年1月12日。台灣大學。, Jan, 2019.
27. 吳聖韓、龔紘毅*, "新穎羅非魚顆粒蛋白胜肽GRN-41具有免疫調節及抗菌活性," 2018海峽科技專家論壇 海峽兩岸海洋漁業可持續發展研討會, 2018年6月10日~11日。福建廈門集美大學。, Jun, 2018.
28. 蘇昱溥、朱珮云、林育禾、龚紘毅、黄章文*, "神仙鱼(Pterophyllum scalare) RNA-Seq de novo 组装和差异性转录组分析," 2018海峽科技專家論壇 海峽兩岸海洋漁業可持續發展研討會, 2018年6月10日~11日。福建廈門集美大學。, Jun, 2018.
29. 李嘉衔、曾冠霖、朱珮云、刘俊宏、龚紘毅、黄章文*, "从低温转录组数据库开发尖吻鲈耐寒性状功能基因标记," 2018海峽科技專家論壇 海峽兩岸海洋漁業可持續發展研討會, 2018年6月10日~11日。福建廈門集美大學。, Jun, 2018.
30. 曾柏鈞、戴澤宇、 林峰右、 葉信利、 黄章文、龔紘毅*, "龍膽石斑之成長相關功能性基因分子標誌開發," 2018海峽科技專家論壇 海峽兩岸海洋漁業可持續發展研討會, 2018年6月10日~11日。福建廈門集美大學。, Jun, 2018.
31. 徐德華*、龔紘毅、黄章文, "台湾沿岸海域增殖放流-基因多樣性標準的建立," 2018海峽科技專家論壇 海峽兩岸海洋漁業可持續發展研討會, 2018年6月10日~11日。福建廈門集美大學。, Jun, 2018.
32. 李嘉銜、徐翎、徐德華、龔紘毅、黄章文*, "利用轉錄组數據庫建立尼羅羅非鱼耐寒相關之遗傳標誌," 2018海峽科技專家論壇 海峽兩岸海洋漁業可持續發展研討會, 2018年6月10日~11日。福建廈門集美大學。, Jun, 2018.
33. 龔紘毅, "Mozambique tilapia granulin peptide OmGRN-41 is an immune modulator and a novel antimicrobial peptide against marine Vibrio spp.," 中研院、中山大學與臺灣海洋大學海洋生物科技博士學位學程三方研討會。2018/05/07。中山大學海洋科學院。高雄。, May, 2018.
34. 龔紘毅, "Fish Transgenesis and Genome Editing- Applications in Model Fish (Zebrafish), Ornamental Fish (Pink Angelfish) and Bioreactor (Tilapia)," 國立東華大學生科系專題演講。2018年6月1日。, May, 2018.
35. 龔紘毅*、黃章文、陸振岡, "羅非(吳郭)魚抗病分子機理及良種選育研究," 2018年度海峽兩岸水產生物資源領域學術交流研討會, 中國科學院水生生物研究所。2018年4月9-13日。中國武漢。, Apr, 2018.
36. 蘇昱溥、朱珮云、林育禾、龔紘毅、黃章文*, "建立神仙魚轉錄體基因庫與顯色性狀功能性基因標記 (Development of Color Trait Related Functional Gene-Based Markers Based on Transcriptome Database in Freshwater Angelfish (Pterophyllum scalare))," 臺灣水產學會107年度會員大會及學術論文發表會。107年1月19日。高雄海洋科技大學。, Jan, 2018.
37. 李嘉銜、徐翎、徐德華、龔紘毅、黃章文*, "由次世代定序建立之尼羅吳郭魚轉錄體資料庫開發耐寒相關之微衛星與單一核苷酸多型性標誌(Cold Tolerance Gene-Associated SSRs and SNPs Discovery from the Transcriptome Database by Next Generation Sequencing in Nile Tilapia (Oreochromis niloticus))," 臺灣水產學會107年度會員大會及學術論文發表會。107年1月19日。高雄海洋科技大學。, Jan, 2018.
38. 許禮陽、朱珮云、邱宇廷、曾柏鈞、林峰右、葉信利、戴昆財、龔紘毅、黃章文*, "不同石斑魚種間微衛星 DNA 之遺傳變異分析(Microsatellite DNA Analysis of Genetic Variation between Different Grouper Species)," 臺灣水產學會107年度會員大會及學術論文發表會。107年1月19日。高雄海洋科技大學。, Jan, 2018.
39. 邱宇廷、許禮陽、林峰右 葉信利、戴昆財、龔紘毅、黃章文*, "新型雜交石斑魚分子育種之線粒體與微衛星標記遺傳資訊(Mitochondrial and Microsatellite Markers Analysis Reveals the Genetic Information for Molecular Breeding in A Novel Grouper Hybrid)," 臺灣水產學會107年度會員大會及學術論文發表會。107年1月19日。高雄海洋科技大學。, Jan, 2018.
40. 徐德華、龔紘毅、黃章文, "應用貝氏類聚法判別 3 種台灣主要放流鯛魚之來源 (Source identification of three major Sparidae fishes by using Bayesian clustering method for stock enhancement in Taiwan)," 臺灣水產學會107年度會員大會及學術論文發表會。107年1月19日。高雄海洋科技大學。, Jan, 2018.
41. 龔紘毅*、黃章文, "吳郭魚(羅非魚)抗病育種之分子標誌開發," 第七屆臺閩水產學術論文研討會。創新水產生物科技- 養殖與休閒漁業。臺灣海洋大學。2017年11月13日。, Nov, 2017.
42. 黃章文、龔紘毅, "肌肉增長基因標誌開發與優質石斑魚輔助選育應用," 第七屆臺閩水產學術論文研討會。創新水產生物科技- 養殖與休閒漁業。臺灣海洋大學。2017年11月13日。, Nov, 2017.
43. 龔紘毅, "羅非魚之宿主防衛基因研究及標誌輔助育種之抗病分子標誌開發。," 第八屆海峽兩岸魚類生理與養殖研討會。2017年10月22日~25日。廈門大學。廈門,中國。, Oct, 2017.
44. 龔紘毅, "新穎中型螢光魚(粉紅神仙魚)及台灣鯛生物反應器開發與產學合作," 台灣新農業亮點系列(I) 經驗分享及產業推動。106年9月4日至5日。, 科技部生命科學研究推動中心主辦。(Invited speaker), Sep, 2017.
45. Tsai YT, Wu SH, Gong HY*., "Myostatin a and b double knockout causes not only myocyte hyperplasia but also apoptosis and mortality in adult zebrafish," 2017 Annual Meeting of Taiwan Marine Biotechnology Society. Auguat 7, 2017. National Changhua University of Education. P043., Aug, 2017.
46. Ho CH, Pham HN, Wu SH, Tseng PC, Kuo YH, Huang CW, Wu JL, Gong HY., "Gene amplification of antimicrobial peptide hepcidin genes in the genome of Nile tilapia.," 2017 Annual Meeting of Taiwan Marine Biotechnology Society. August 7, 2017. National Changhua University of Education. P047., Aug, 2017.
47. Kuo YH, Chan YH, Tseng PC, Huang CW, Hsu TH, Chiou PP, Liu KM, Lin CH, Gong HY*., "Evaluation of genetic diversity in wild and cultured populations of silver seabream in Taiwan by polymorphic DNA markers.," 2017 Annual Meeting of Taiwan Marine Biotechnology Society. Auguat 7, 2017. National Changhua University of Education. P018., Aug, 2017.
48. Hsu LY, Chu PY, Zeng KL, tseng PC, Kuo YH, Lin FY, Yeh SL, Tai KT, Chou HY, Gong HY*, Huang CW*., "Establishment of molecular marker-assisted selection by adoption of RFID tagging technology for genetics management i the broodstock of giant grouper (Epinephelus lanceolatus)," 2017 Annual Meeting of Taiwan Marine Biotechnology Society. Auguat 7, 2017. National Changhua University of Education. P017., Aug, 2017.
49. Chen HT, Ken CF, Gong HY, Wu JL*., "Development of a new generation humanized fish scale-derived type I collagen matrix: A preliminary study using zebrafish as a model," 2017 Annual Meeting of Taiwan Marine Biotechnology Society. Auguat 7, 2017. National Changhua University of Education. P10., Aug, 2017.
50. Li JX, Chu PY, Hsu L, Kuo YH, Gong HY, Huang CW*., "Cold tolerance gene-associated SSRs and SNPs discovery form the transcriptome database by next generation sequencing in Nile tilapia (Oreochromis niloticus)," 2017 Annual Meeting of Taiwan Marine Biotechnology Society. Auguat 7, 2017. National Changhua University of Education. P28., Aug, 2017.
51. Chu PY, Chien TC, Zeng KL, Tu ZZ, Liu PC, Nan FH, Lin CH, Gong HY, Huang CW*., "Evaluation of microsatellite DNA markers for genetic diversity analysis in the wild and fry hatchery-released populations of yellowfin seabream (Acanthopagrus latus)," 2017 Annual Meeting of Taiwan Marine Biotechnology Society. Auguat 7, 2017. National Changhua University of Education., Aug, 2017.
52. Zeng KL, Chu PY, Tu CC, Nan FH, Lin CH, Gong HY, Huang CW*., "Development of gene-based SSR markers related to cold stress in Asian seabass (Lates calcarifer) based on transcriptome database," 2017 Annual Meeting of Taiwan Marine Biotechnology Society. Auguat 7, 2017. National Changhua University of Education. P26., Aug, 2017.
53. Hong-Yi Gong*, Yi-Ting Tsai, Sheng-Han Wu, Chia-Hui Ho, Ching-Yu Huang, Shin-Jie Huang, Chuen-Fu Ken, Jyh-Yih Chen, Jen-Leih Wu, "Establishment of transgenic tilapia expressing Atlantic salmon ∆6- and ∆5-desaturases in muscle and liver to enhance survivability against Streptococcus iniae.," Innovative Translational Agricultural Research Program (ITAR) Conference & Exhibition. 18th~20th July, 2017. Academia Sinica, Nankang, Taipei., Jul, 2017.
54. 龔紘毅, "Regulation of skeletal muscle growth in zebrafish model and application in Nile tilapia.," 2017中研院、中山大學及海洋大學之海洋生物科技博士學位學程學術研討會。2017年6月16日。臺灣海洋大學。, Jun, 2017.
55. Hong-Yi Gong (Invited speaker), "Transgenesis and Genome Editing in Teleost Fish- from Zebrafish, Angelfish to Tilapia.," International Program in Ornamental Fish Science and Technology (觀賞魚科技國際學位專班), National Pingtung University of Science and Technology (NPUST), Pingtung, Taiwan, Apr, 2017.
56. 何佳蕙、吳聖韓、龔紘毅*, "新穎尼羅吳郭魚肝臟抗菌肽HAMP3基因被強烈活化表現以對抗鏈球菌感染," 台灣水產學會105年度會員大會暨學術論文發表會。2017年1月17日。臺灣海洋大學。, Jan, 2017.
57. 蔡宜庭、吳聖韓、龔紘毅*, "以CRISPR/Cas9技術建立肌肉生長抑制素a與b雙基因剔除之肌肉增生斑馬魚," 台灣水產學會105年度會員大會暨學術論文發表會。2017年1月17日。臺灣海洋大學。, (榮獲水產生物技術領域第一名), Jan, 2017.
58. 郭怡萱、張瑜軒、曾柏鈞、徐德華、黃章文、邱品文、劉光明、林正輝、龔紘毅*, "以多型性微衛星DNA標誌評估台灣黃錫鯛野生與養殖族群之遺傳多樣性," 台灣水產學會105年度會員大會暨學術論文發表會。2017年1月17日。臺灣海洋大學。, Jan, 2017.
59. 吳聖韓、陳歷歷、龔紘毅*, "鑑定與吳郭魚顆粒蛋白 GRN-41 交互作用之分子並探討其調控免疫之分子機制," 台灣水產學會105年度會員大會暨學術論文發表會。2017年1月17日。臺灣海洋大學。, (榮獲水產疾病及微生物領域第三名), Jan, 2017.
60. 郭怡萱、朱珮云、曾冠霖、伍宇芳、冉繁華、林正輝、龔紘毅、黃章文, "建立尼羅吳郭魚 (Oreochromis niloticus) 耐寒基因微衛星資料庫," 台灣水產學會105年度會員大會暨學術論文發表會。2017年1月17日。臺灣海洋大學。, Jan, 2017.
61. 徐德華*、龔紘毅、黃章文, "應用轉錄組定序開發烏賊跨物種之通用微衛星標記," 台灣水產學會105年度會員大會暨學術論文發表會。2017年1月17日。臺灣海洋大學。, Jan, 2017.
62. 陳漢唐、耿全福、龔紘毅、吳金洌*, "擬人化吳郭魚鱗在新世代生物眼角膜之開發與應用," 台灣水產學會105年度會員大會暨學術論文發表會。2017年1月17日。臺灣海洋大學。, (榮獲壁報論文水產生物技術組第二名), Jan, 2017.
63. 伍宇芳、曾冠霖、朱珮云、林庭宏、冉繁華、劉俊宏、林正輝、 龔紘毅、黃章文*, "金目鱸轉錄體定序之數位化基因表現分析," 台灣水產學會105年度會員大會暨學術論文發表會。2017年1月17日。臺灣海洋大學。, (榮獲壁報論文水產生物技術組佳作), Jan, 2017.
64. 龔紘毅、黃章文、陸振岡。, "魚類抗病的分子機理及良種選育研究(兩岸合作研究)- 吳郭魚抗病基因及抗鏈球菌育種研究。," 海峽兩岸水產生物資源中期學術交流研討會。2016年12月6日。台灣海洋大學。, Dec, 2016.
65. Tsai YT, Wu SH and Gong HY*, "Single and Double Knockout of Myostatin a and b Genes in Zebrafish by CRISPR/Cas9," 2016 Taiwan Zebrafish Symposium. 2016/12/3. NHRI, Dec, 2016.
66. Gong HY*, Tseng PC, Wei ZZ, Huang SC, Wu SH, Chin HY, Ho CH, Lin WF, Tsai YT, Kuo YH, Lin CC, Huang MC, Chou CH, Wu JL., "Establishment of fast-growing transgenic tilapia as a bioreactor for the production of fish scale-derived artificial biocorneas.," 2016臺灣海洋生物技術學會海洋生技與產業發展研討會。105年7月22日,國立臺灣海洋大學。, Jul, 2016.
67. Lin WF, Wu SH, Huang CW, Gong HY*., "Short-form progranulin 2 (PGRN2) of Nile tilapia modulates innate immune-related genes against Streptococcus.," 2016臺灣海洋生物技術學會海洋生技與產業發展研討會。105年7月22日,國立臺灣海洋大學。, (壁報論文競賽榮獲碩士及大專組佳作), Jul, 2016.
68. Tsai YT, Wu SH, Gong HY*., "Establishment of myostatin a and b single and double gene knockout zebrafish by CRISPR/Cas9 technology.," 2016臺灣海洋生物技術學會海洋生技與產業發展研討會。105年7月22日,國立臺灣海洋大學。, (壁報論文競賽榮獲碩士及大專組第三名), Jul, 2016.
69. Ho CH, Wu SH, Gong HY*., "Three hepatic antimicrobial peptide HAMP genes were strongly activated in liver, spleen, head kidney and gill in Nile tilapia to defend against virulent Streptococcus iniae.," 2016臺灣海洋生物技術學會海洋生技與產業發展研討會。105年7月22日,國立臺灣海洋大學。, Jul, 2016.
70. Chen HT, Ken CF, Gong HY, Wu JL*., "Development of the new generation humanized bio-cornea in tilapia (Oreochromis niloticus)," 2016臺灣海洋生物技術學會海洋生技與產業發展研討會。105年7月22日,國立臺灣海洋大學。, Jul, 2016.
71. 邱士辰 龔紘毅 吳金洌 耿全福。, "海洋生物技術應用於眼角膜醫材與美白研究。," 2016臺灣海洋生物技術學會海洋生技與產業發展研討會。105年7月22日,國立臺灣海洋大學。, Jul, 2016.
72. Chu PY, Tsai GS, Wang YH, Huang YS, Gong HY, Huang CW*., "Development and application of gene-based markers-assisted selection in Taiwan ornamental shrimp species.," 2016臺灣海洋生物技術學會海洋生技與產業發展研討會。105年7月22日,國立臺灣海洋大學。, Jul, 2016.
73. 龔紘毅, "羅非(吳郭)魚抗病基因及抗鏈球菌育種研究," 第六屆閩台水產學術研討會。2016海峽兩岸水產增養殖與病害學術研討會。, 福建廈門福建省水產研究所。2016年6月11日。, Jun, 2016.
74. 黃靜怡、朱珮云、林文福、劉富光、 龔紘毅、黃章文*, "建立尼羅吳郭魚種內雜交家系育種之成長標記," 104 年度臺灣水產會員大會暨學術論文發表會。105年1月19日。國立屏東科技大學。, Jan, 2016.
75. 朱珮云、詹惟任、徐翎、孫嘉駿、劉擎華、黃沂訓、龔紘毅、黃章文*, "彩色米蝦轉錄體定序之數位化基因表現分析," 104 年度臺灣水產會員大會暨學術論文發表會。105年1月19日。國立屏東科技大學。, (水產生物技術組- 論文壁報競賽佳作), Jan, 2016.
76. 何佳蕙、吳聖韓、林文福、龔紘毅*, "肝臟抗菌肽HAMPs參與尼羅吳郭魚免疫調節及抗鏈球菌之機制," 104 年度臺灣水產會員大會暨學術論文發表會。105年1月19日。國立屏東科技大學。, Jan, 2016.
77. 關榮豪、許秦源、曾冠霖、呂明偉、徐德華、林正輝、 劉秉忠、冉繁華、龔紘毅、黃章文*, "利用SSR分子標記探討點帶石斑族群遺傳多樣性," 104 年度臺灣水產會員大會暨學術論文發表會。105年1月19日。國立屏東科技大學。, (漁業資源、管理組- 論文壁報競賽第三名), Jan, 2016.
78. 關榮豪、許秦源、曾冠霖、涂智欽、徐德華、林正輝、劉秉忠、冉繁華、龔紘毅、黃章文*, "以微衛星DNA標記建立黃鰭鯛族群遺傳資訊之研究," 104 年度臺灣水產會員大會暨學術論文發表會。105年1月19日。國立屏東科技大學。, Jan, 2016.
79. 曾冠霖、楊衛崟、曾柏鈞、林峰右、葉信利、周信佑、龔紘毅、黃章文*, "龍膽石斑成長性狀之功能性基因標記," 104 年度臺灣水產會員大會暨學術論文發表會。105年1月19日。國立屏東科技大學。, (漁業資源、管理組- 論文壁報競賽佳作), Jan, 2016.
80. 張瑜軒、郭怡萱、 曾柏鈞、吳育慈、林正輝、 劉光明、邱品文、劉秉忠、許秦源、冉繁華、黃章文、龔紘毅*, "開發多型性微衛星DNA標誌評估台灣黃錫鯛養殖與野生族群," 104 年度臺灣水產會員大會暨學術論文發表會。105年1月19日。國立屏東科技大學。, Jan, 2016.
81. 林文福、吳聖韓、何佳蕙、鄭頌雅、龔紘毅*, "尼羅吳郭魚短型顆粒蛋白前體 2 (PGRN2) 基因參與調節先天性免疫," 104 年度臺灣水產會員大會暨學術論文發表會。105年1月19日。國立屏東科技大學。, Jan, 2016.
82. 魏梓傑、黃士晉、胡紹揚、林育禾、吳金洌、龔紘毅*, "以生殖細胞專一性Piwi1啟動子建立基因轉殖魚誘導性不孕技術," 104 年度臺灣水產會員大會暨學術論文發表會。105年1月19日。國立屏東科技大學。, (水產生物技術組- 論文壁報競賽佳作), Jan, 2016.
83. 龔紘毅, "以新穎肌肉表現系統建立螢光神仙魚及生長激素基因轉殖尼羅吳郭魚," 第五屆臺閩水產學術研討會。創新水產生物科技- 藻類與養殖漁業。, 2015年11月16日。國立臺灣海洋大學。(Invited speaker), Nov, 2015.
84. 龔紘毅, "新穎肌肉表現系統之建立及其在粉紅神仙魚與生物反應器之應用," 2015臺灣海洋生物技術學會年會暨「前瞻海洋生物技術系列論壇(II)- 觀賞魚」。p58-62。, 宜蘭大學。104年10月19日。(Invited speaker), Oct, 2015.
85. 黃章文、龔紘毅, "分子標誌輔助選育在水生動物遺傳改良之研究與應用," 中央研究院細胞與個體生物學研究所及臺灣海洋大學生命科學院聯合學術研討會, 104年9月21-22日。臺灣海洋大學。, Sep, 2015.
86. 吳聖韓、林宏傑、龔紘毅*, "Novel tilapia Granulin Peptide GRN-41 is an immune modulator and contribute to against Vibrio vulnificus infection," 103 年度臺灣水產會員大會暨學術論文發表會。104年1月18日。台灣大學。, 榮獲2015年學術論文海報比賽「疾病、微生物組」最佳論文, Jan, 2015.
87. 盧佳彣、吳聖韓、王涵青、 陳建初、龔紘毅*, "南美白對蝦長型 PGRN在 WSSV 感染後期被強烈誘導表現," 103 年度臺灣水產學會會員大會暨學術論文發表會。104年1月18日。台灣大學。, 榮獲2015年學術論文海報比賽「疾病、微生物組」佳作, Jan, 2015.
88. 劉興懿、朱永桐、呂明偉、 龔紘毅、邱品文、周信佑*, "開發抗兩種石斑魚虹彩病毒之多重相乳化口服 DNA 疫苗," 103 年度臺灣水產學會會員大會暨學術論文發表會。104年1月18日。台灣大學。, Jan, 2015.
89. 朱珮云、黃靜怡、張僑耘、 郭建賢、劉富光、龔紘毅、 周信佑、黃章文*, "性別決定基因輔助開發尼羅吳郭魚性別鑑定標記," 103 年度臺灣水產學會 會員大會暨學術論文發表會。104年1月18日。台灣大學。, Jan, 2015.
90. 楊衛崟、林峰右、曾柏均、 曾品敦、龔紘毅、周信佑、 黃沂訓、葉信利、黃章文*, "石斑魚種別鑑定與成長性狀之分子標記," 103 年度臺灣水產學會 會員大會暨學術論文發表會。104年1月18日。台灣大學。, Jan, 2015.
91. 黃靜怡、朱珮云、楊衛崟、 袁書涵、關榮豪、紀璟叡、 吳志律、劉富光、龔紘毅、 黃章文*, "基因表現輔助吳郭魚成長分子標記之建立," 103 年度臺灣水產學會 會員大會暨學術論文發表會。104年1月18日。台灣大學。, Jan, 2015.
92. 魏梓傑、黃士晉、曾柏鈞、 林文福、黃信傑、吳金洌、 何佳蕙、吳聖韓、龔紘毅*, "建立肌肉及肝臟專一性表現基因轉殖尼羅吳郭魚," 103 年度臺灣水產學會 會員大會暨學術論文發表會。104年1月18日。台灣大學。, Jan, 2015.
93. 林文福、吳聖韓、林宏傑、何佳蕙、黃章文、龔紘毅*, "吳郭魚參與抗病之短型顆粒蛋白前體基因擴增研究," 103 年度臺灣水產學會 會員大會暨學術論文發表會。104年1月18日。台灣大學。, Jan, 2015.
94. 曾柏鈞、林峰右、戴澤宇、 吳育甄、楊衛崟、楊甦牧、 張怡慧、林文福、黃靜怡、 黃章文、周信佑、葉信利、吳金洌、 龔紘毅*, "肌肉生長相關基因3端未轉譯區之多型性微衛星DNA及小段缺失 DNA 可做龍膽石斑育種之分子標誌," 103 年度臺灣水產學會 會員大會暨學術論文發表會。104年1月18日。台灣大學。, Jan, 2015.
95. 吳育甄、林峰右、龔紘毅、 黃章文、葉信利, "MEF2D 基因在龍膽石斑魚鱗片 RNA 表現量 之研究," 103 年度臺灣水產學會 會員大會暨學術論文發表會。104年1月18日。台灣大學。, Jan, 2015.
96. Wang YD, Huang SJ, Chou HN, Liao WL, Gong HY, Chen JY*., "Transcriptome analysis of the effect of Vibrio alginolyticus infection on the innate immunity-related complement pathway in Epinephelus coioides," 2014 Annual Meeting for The Fisheries Society of Taiwan. Jan. 18, 2015. National Taiwan Univerity, Taipei, Taiwan., Jan, 2015.
97. Wang YL, Chen CC, Gong HY*, "Studies on roles of Akirin2 in liver during embryonic development and adult zebrafish," 2014 Annual Meeting for the Fisheries Society of Taiwan. Jan. 18, 2015. National Taiwan Univerity, Taipei, Taiwan., Jan, 2015.
98. 龔紘毅*,黃章文,周信佑, "以功能性基因相關分子標誌進行石斑魚之成長與抗病育種," 臺灣海洋大學103年度海洋中心成果發表會。104年1月13日。, Jan, 2015.
99. Gong HY*, Huang SC, Wu SH, Chen MC, Lin YH, Wu JL., "A Novel Muscle-Specific Enhancer/Promoter Expression System Can Be Used in Establishment of Fluorescent Ornamental Fish and Transgenic Tilapia," 2014 臺灣海洋生物技術學會第二屆會員大會暨學術研討會。成功大學,台南。103年12月6~7日。P002。, Dec, 2014.
100. Gong HY*, Chin HY, Wei ZJ, Tseng PC, Lin WF, Ho CH, Wu SH, Huang SC., "Muscle-specific expression of grouper GH in zebrafish and tilapia enhances growth phenotype.," 2014 Taiwan Zebrafish Symposium 台灣斑馬魚研討會。Dec. 13, 2014。國家衛生研究院。P40。, Dec, 2014.
101. Hong-Yi Gong, "Novel muscle-specific promoter and strong intronic enhancer identified from zebrafish ckmb gene was used in transgenic zebrafish, angelfish and tilapia and their applications ," 2014 Taiwan Zebrafish PI Meeting. Aug.1, 2014. Academia Sinica, Taipei., (Oral), Aug, 2014.
102. Chang CY, Sung YC, Gong HY, Huang CW, Wu JL., "MicroRNA-133 modulates skeletal muscle hypertrophy through binding JunB transcription factor in zebrafish.," 2014 臺灣發育生物學研討會。Aug 15-16,臺灣海洋大學,基隆。, Aug, 2014.
103. 黃士晉、林育禾、陳鳴泉、吳金洌、龔紘毅*, "基因轉殖粉紅神仙魚品系之建立及鑑定," 102年度台灣水産年會。2014年1月18日。嘉義大學。, Jan, 2014.
104. 楊衛崟、林峰右、楊甦牧、龔紘毅、周信佑、葉信利、黃章文*, "龍膽石斑多態性DNA標記之成長性狀分析," 102年度台灣水産年會。2014年1月18日。嘉義大學。, Jan, 2014.
105. 林寶凱、康兆凱、龔紘毅、李宗翰*, "鑑定魚類微絨毛蛋白的啟動子:建立腸道專一表現的螢光稻田魚," 102年度台灣水産年會。2014年1月18日。嘉義大學。, Jan, 2014.
106. 朱珮云、黃靜怡、林文福、龔紘毅、劉擎華、黃沂訓、黃章文*, "粒線體DNA D-loop區域應用於吳郭魚品種鑑定," 102年度台灣水産年會。2014年1月18日。嘉義大學。, Jan, 2014.
107. 林峰右*、吳育甄、龔紘毅、葉信利, "建立龍膽石斑育種評估指標," 102年度台灣水産年會。2014年1月18日。嘉義大學。, Jan, 2014.
108. Tang CSN, Wu SH, Chin HY, Gong HY*, "Zebrafish innate immunity was modulated by a novel tilapia short form progranulin.," 2013 Taiwan Zebrafish Meeting. Dec.7. Academia Sinica. Taipei, Taiwan., 榮獲壁報展示優勝, Dec, 2013.
109. 龔紘毅, "新穎魚類肌肉專一性增強子/啟動子在建立基因轉殖螢光觀賞魚及其他應用潛力," 第七屆海峽兩岸魚類生理及養殖研討會, 上海海洋大學,中國。2013年11月1日~5日。, Nov, 2013.
110. 周信佑, 劉興懿,朱永桐,呂明偉,龔紘毅, "兩種石斑魚虹彩病毒之口服DNA疫苗研發," 第七屆海峽兩岸魚類生理及養殖研討會, 上海海洋大學,中國。2013年11月1日~5日。, Nov, 2013.
111. 黃章文,張巧宜,龔紘毅,吳金洌, "微核醣核酸-133與JunB基因表現調節尼羅吳郭魚(Oreochromis nilotica)骨骼肌成長," 第七屆海峽兩岸魚類生理及養殖研討會, 上海海洋大學,中國。2013年11月1日~5日。, Nov, 2013.
112. Clare Siing-Nga Tang, Sheng-Han Wu, Hui-Yen Chin, Hong-Yi Gong*., "Regulation of Innate Immunity by Novel Tilapia Short Form Progranulin in Transgenic Zebrafish.," 臺灣海洋生物技術學會(Taiwan Marine Biotechnology Society)第一屆會員大會暨學術研討會。中華民國102年11月30日。P92。, 論文競賽碩士組第二名。, Nov, 2013.
113. Chang CY, Sung YC, Huang CW, Gong HY, Wu JL., "MicroRNA-133 modulates skeletal muscle hypertrophy through binding JunB transcription factor in zebrafish.," 臺灣海洋生物技術學會(Taiwan Marine Biotechnology Society)會員大會暨學術研討會。中華民國102年11月30日。P69。, Nov, 2013.
114. Chung WJ, Gong HY, Hu SY., "Production of biologically active recombinant grouper (Epinephelus lanceolatus) growth hormone from E. coli.," 臺灣海洋生物技術學會(Taiwan Marine Biotechnology Society)會員大會暨學術研討會。中華民國102年11月30日。P74。, Nov, 2013.
115. 龔紘毅, "新穎魚類肌肉專一性增強子/啟動子在建立基因轉殖螢光觀賞魚及其他應用潛力 (Novel Teleost Muscle-Specific Enhancer/Promoter Used in Establishment of Transgenic Fluorescent Ornamental Fish and Other Application Potential)," 農委會水產試驗所總所演講(invited speaker), 5/31/2013, May, 2013.
116. 劉興懿、呂明偉、龔紘毅、周信佑, "開發抗兩種石斑魚虹彩病毒之多重相乳化口服DNA疫苗," 101年度台灣水產年會(The Fisheries Society of Taiwan 2012 Annual Meeting)。2013年1月19日。臺灣海洋大學。p87。(poster), Jan, 2013.
117. 龔紘毅*(Gong, Hong-Yi),戴澤宇,林峰右,黃章文,陳韋燕,葉信利,吳金洌,周信佑, "開發龍膽石斑標誌輔助育種之生長相關多型性微衛星DNA標誌 (Development of growth-associated polymorphic microsatellite DNA markers for marker-assisted selection of giant grouper)," 101年度台灣水產年會(The Fisheries Society of Taiwan 2012 Annual Meeting)。2013年1月19日。臺灣海洋大學。p66。(oral), Jan, 2013.
118. 黃士晉、林育禾、陳鳴泉、王韵倫、吳金洌、龔紘毅*(Gong, Hong-Yi), "開發以新穎肌肉增強子/啟動子表現台灣軸孔珊瑚紅色螢光蛋白之基因轉殖粉紅神仙魚 (Development of transgenic pink angelfish expressing Taiwan Acropora coral red fluorescent proteins by novel muscle-specific enhancer/promoter)," 101年度台灣水產年會 (The Fisheries Society of Taiwan 2012 Annual Meeting)。2013年1月19日。臺灣海洋大學。p174。(poster), Jan, 2013.
119. 鄭頌雅、吳聖韓、陳韋燕、吳金洌、龔紘毅* (Gong, Hong-Yi), "吳郭魚長型與短型PGRN調控魚類生長及免疫之功能性研究 (Functional study of tilapia long and short form PGRN in the regulation of fish growth and immunity)," 101年度台灣水產年會(The Fisheries Society of Taiwan 2012 Annual Meeting)。2013年1月19日。臺灣海洋大學。p164。(poster), ("水產生物技術組"壁報比賽第二名), Jan, 2013.
120. 陳韋燕、龔紘毅* (Gong, Hong-Yi), "肌肉專一性表現龍膽石斑生長激素在基因轉殖斑馬魚之功能性研究 (Functional Studies of Muscle-specific Expression of Giant Grouper’s Growth Hormone in Transgenic Zebrafish)," 101年度台灣水產年會 (The Fisheries Society of Taiwan 2012 Annual Meeting)。2013年1月19日。臺灣海洋大學。p175。(poster), Jan, 2013.
121. 黃章文, 龔紘毅, 吳金洌, "吳郭魚微核醣核酸調控肌肉生長之機制及標記輔助選種之應用," 第六屆海峽兩岸魚類生理及養殖研討會。11月23日~28日。廣州,中國。, Nov, 2012.
122. 戴澤宇,林峰右,陳韋燕,黃章文,葉信利,吳金洌,林慶君, 周信佑,龔紘毅*, "龍膽石斑骨骼肌生成控制功能性基因cDNAs之多型性DNA標誌與其生長性狀顯著相關," 第六屆海峽兩岸魚類生理及養殖研討會。11月23日~28日。廣州,中國。, Invited speaker, Nov, 2012.
123. Chen, C. C., Li, Y. H, Wu, J. L., Kawakami, K., and Gong, H. Y.*, "Akirin1 and Akirin2 antagonistically regulate muscle hypertrophy in zebrafish.," The 27th Joint Annual Conference of Biomedical Sciences. March 17-18, 2012. P465., Mar, 2012.
124. Li, Y. H., Hu, S. Y., Liao, J. S., Chen, S. Y., Gong, H. Y., Lee, Y. W., Lin, G. H., Wu, S. Y., Chiang, K. Y., and Wu, J. L.*, "Progranulin is required for muscle progenitor cell proliferation in zebrafish postembryonic muscle growth.," The 27th Joint Annual Conference of Biomedical Sciences. March 17-18, 2012. P572., Mar, 2012.
125. Wu, S. H., Lin, H. J., Chin, H. Y., Li, Y. H., Huang, C. Y., Wu, J. L., Gong, H. Y.*, "Novel Tilapia granulin peptide GRN-41 is a strong innate immune modulator.," The 27th Joint Annual Conference of Biomedical Sciences. March 17-18, 2012. P934., Mar, 2012.
126. Liu, W., Chen, J. R., Hsu, J. H., Chen, Y. M., Lin, J. Y, Huang, S. J., Gong, H. Y., Li, Y. H., lin, C. C., Chang, Z. K., Chen, Y. C., Lin, C., Kawakami, K., Wu, J. L.*, "Involvement of TGF-beta1 in intrahepatic cholangiocarcinoma formation using HBx and HCV core dual transgenic zebrafish as a model.," The 27th Joint Annual Conference of Biomedical Sciences. March 17-18, 2012. P956., Mar, 2012.
127. Tai, T. Y., Chin, H. Y. and Gong, H. Y.*, "龍膽石斑轉錄體微衛星序列分析應用於分子標誌輔助育種(Type I Microsatellites Analysis of Giant Grouper Transcriptomes for the Application in Marker-Assisted Breeding)," The Fisheries Society of Taiwan 2011 Annual Meeting. 中山大學。BP-02., Dec, 2011.
128. Chin, H. Y., Tai, T. Y. and Gong, H. Y.*, "Establishment of Growth-Related Genes and Type I Microsatellites of Giant Grouper as Functional Biomarkers for Marker Assisted Breeding.," The Fisheries Society of Taiwan 2011 Annual Meeting. BP-03., Dec, 2011.
129. Wu, S. H., Lin, H. J. and Gong, H. Y.*, "Molecular mechanism and application of tilapia Granulins in the regulation of innate immunity of teleost.," The Fisheries Society of Taiwan 2011 Annual Meeting. BP-07., Dec, 2011.
130. Huang, C. W., Hu, S. Y., Li, Y. H., Chi, J. R., Lin, G. H., Lin, C. C., Gong, H. Y., Wang, W. L., Chen, R. H., Chang, S. J., Liu, F. G., and Wu, J. L.*, "A systematic analysis of the skeletal muscle growth-related microRNA transcriptome in tilapia.," The Fisheries Society of Taiwan 2011 Annual Meeting. BPN-06., Dec, 2011.
131. Chen, C. C., Li, Y. H, Wu, J. L., Kawakami, K., and Gong, H. Y.*, "Functional study of Akirin gene family on the regulation of muscle hypertrophy in zebrafish.," 2011 Taiwan Zebrafish Symposium. Dec. 10, 2011. ICOB, Academia Sinica. P41., Dec, 2011.
132. 龔紘毅*, "A novel tilapia granulin GRN-41 activates innate immunity genes.," 第五屆海峽兩岸魚類生理與養殖研討會。2011年11月2-4日。中研院細胞與個體生物學研究所。(invited speaker), Nov, 2011.
133. Gong, Hong-Yi, "Transgenic fluorescent fish established by muscle-specific TetOff system to study functional genes of muscle and its application.," Symposium on Biotechnology Development in Taiwan. May 31, 2011. Tainan, National Cheng Kung University. (invited speaker), May, 2011.
134. Huang, C. C., Chang, Z. K., Chen, J. R., Lin, M. Y., Li, Y. H., Liu, W., Lin, G. H., Lin, C. C., Gong, H. Y. *, and Wu, J. L.*, "Initiation mechanism of intrahepatic cholangiocarcinoma progression in liver-specific IMP2 transgenic zebrafish.," The 26th Joint Annual Conference of Biomedical Sciences. P74., Mar, 2011.
135. Wang, W. L., Hong, J. R., Lin, G. H., Liu, W., Gong, H. Y., Lu, M. W., and Wu, J. L.*, "Expression of TNFα regulates Bad/Bid and RIP1/ROS-mediated cell death in birnavirus-infected fish cells.," The 26th Joint Annual Conference of Biomedical Sciences. P241., Mar, 2011.
136. Wang, W. L., Liu, W., Gong, H. Y., Hong, J. R., Lin, C. C. and Wu, J. L.*, "Activation of cytokines expression occurs through the TNFα/NF-κB-mediated pathway in birnavirus infected cells.," The 26th Joint Annual Conference of Biomedical Sciences. P242., Mar, 2011.
137. Hsu, C. H., Liu, W., Chen, J. R., Chen, Y. M., Lin, C. Y., Huang, S. J., Gong, H. Y., Li, Y. H., Lin, C. C., Kawakami, K., Wu, J. L.*, "Generation of ICC model by co-expression of HCV core and HBV X in zebrafish liver.," The 26th Joint Annual Conference of Biomedical Sciences. P789., Mar, 2011.
138. Gong, H. Y.*, Huang, C. C., Chang, Z. K., Chen, J. R., Hu, M. C., Lin, M. Y., Li, Y. H., Lu, W., Lin, G. H., Lin, C. C., Kawakami, K., Wu, J. L.*, "Zebrafish model of intrahepatic cholangiocarcinoma induced by liver-specific overexpression of IGF2 mRNA-binding protein 2 (IGF2BP2).," 2010 Taiwan Zebrafish Symposium. Dec. 4, 2010. NHRI. Zhunan, Taiwan. (oral report), Dec, 2010.
139. Gong, H. Y.*, Huang, S. C., Hu, M. C., Chen, C. C., Chen, M. C., Kawakami, K., and Wu, J. L., "Promoter/enhancer analysis of zebrafish muscle-type creatine kinase b (CKMb) gene and its application in fluorescent transgenic ornamental fish.," Annual Meeting of the Fisheries Society of Taiwan. FO-14., Dec, 2010.
140. Huang, C. C., Chang, Z. K., Chen, J. R., Lin, M. Y., Li, Y. H., Liu, W., Lin, G. H., Lin, C. C., Gong, H. Y.*, and Wu, J. L.*, "Initiation mechanism of intrahepatic cholangiocarcinoma progression in liver-specific IMP2 transgenic zebrafish.," Annual Meeting of the Fisheries Society of Taiwan. FO-16., Dec, 2010.
141. Chen, C. C., Wu, J. L., Chen, M. C., Kawakami, K., and Gong, H. Y.*, "Study on molecular mechanism of Akirin1 in the regulation of muscle of teleosts.," Annual Meeting of the Fisheries Society of Taiwan. FP-15., Dec, 2010.
142. Chang Z. K., Gong, H. Y. *, Chen, J. R., Liu, W., Wu, J. L.*, "Co-expression of IMP1 and cMyc induce giant nucleus cell and steatosis in zebrafish liver.," The 25th Joint Annual Conference of Biomedical Sciences. P539., Mar, 2010.
143. Huang, C.C., Chang, Z.K., Chen, J.R., Lin, M.Y., Li, Y.H., Liu, W., Lin, G.H., Lin, C.C., Gong, H.Y.* and Wu, J. L.*, "Initiation mechanism of intrahepatic cholangiocarcinoma progression in liver-specific IMP2 transgenic zebrafish.," The Taiwan Society for Biochemistry and Molecular Biology, P59., Jan, 2010.
144. Lin, H. J., Hu, S. Y., Chen, M. H. C., Huang, C. W., Li, Y. H., Liu, W., Wu, J. L., Gong, H. Y.*, "Establishment of transgenic fish with muscle-specific and inducible expression of tilapia progranulins to study their functions in growth promotion and innate immunity of teleost.," Annual Meeting of the Fisheries Society of Taiwan. BP-05. p222., Dec, 2009.
145. Huang, S. C., Hu, M. C., Chen, C. C., Lin, C. H., Wu, J. L., Chen, M. C., Gong, H. Y.*, "Establishment of transgenic fluorescent fish expressing novel Taiwan Acropora fluorescent proteins by teleost muscle-specific promoter.," Annual Meeting of the Fisheries Society of Taiwan. BP-24. p241., Dec, 2009.
146. Chang, Z. K., Gong, H. Y.*, Chen, J. R., Huang, C. C., Lin, G. H., Liu, W., Wu, J. L.*, "A new CCA model: the induced CCA formation in zebrafish by liver-specific overexpression of IMP2.," Annual Meeting of the Fisheries Society of Taiwan. BP-14. p231., Dec, 2009.
147. Huang, C. W., Hu, S. Y., Li, Y. H., Chi, J. R., Liao, C. H., Lin, G. H., Gong, H. Y., Liu, W., Wang, W. L., Chen, R. H., Chang, S. J., Liu, F. G., Wu, J. L.*, "Growth-Related Genes and MicroRNAs Profiling Reveals Distinct Signatures in N1 and N2 Inbred Lines of Nile Tilapia (Oreochromis niloticus).," Annual Meeting of the Fisheries Society of Taiwan. BPN-09. p254., Dec, 2009.
148. Gong, H. Y.*, Chang, Z. K., Chen, J. R., Hu, M. C., Liu, W., Huang, S. J., Hu, S. Y., Lin, G. H., Kawakami, K., Wu, J. L.*, "Liver-specific overexpression of a novel oncogene IMP2 induces cholangiocarcinoma in zebrafish.," 2009 The 14th Taiwan Joint Cancer Conference., May, 2009.
149. Chang, Z. K., Gong, H. Y.*, Lin, M. Y., Liu, W., Wu, J. L.*, "Transgenic zebrafish model of IGF2 mRNA-binding protein 1 (IMP1) overexpression in liver tumorgenesis.," The 24th Joint Annual Conference of Biomedical Sciences.P300., Mar, 2009.
150. Gong, H. Y.*, Chang, Z. K., Chen, J. R., Lin, M. Y., Lin, G. H., Huang, H. P., Liu, W., Hu, S. Y., Hu, M. C., Huang, S. J., Kawakami, K., Wu, J. L.*, "Liver-specific overexpression of IMP2 induced liver tumor in transgenic zebrafish.," The 24th Joint Annual Conference of Biomedical Sciences. O40., Mar, 2009.
151. Gong, H. Y.*, Lin, W. Z., Chang, Z. K., Liu, W., Wu, J. L.*, "Regulation and functional studies of zebrafish sterol regulatory element binding protein-1 (SREBP-1) gene.," Annual Meeting of the Fisheries Society of Taiwan. P128., Dec, 2008.
152. Gong, H. Y., Lin, M. Y., Huang, S. P., Lin, G. H., Lin, C. J. F., Hu, M. C., Liu, W. T., Huang, S. J., Hu, S.Y., Lin, W. Z., Wang, W. L., Kawakami, K. and Wu, J. L.*, "Roles of IMP2/IGF2BP2 of Human Nonalcoholic Fatty Liver Disease in Zebrafish Model.," 2008 The 13th Taiwan Joint Cancer Conference., May, 2008.
153. Lin, C. J. F., Gong, H. Y., Chen, Y. D., Huang, W. T., Huang, H. P., Chen, M. H. C., Weng, C. F., Her, G. M. and Wu, J. L.*, "Liver-specific overexpression of IGF2b associated with hepatomegaly and steatosis in zebrafish.," 2008 The 13th Taiwan Joint Cancer Conference., May, 2008.
154. Gong, H. Y., Lin, M. Y., Huang, S. P., Lin, G. H., Lin, C. J. F., Hu, M. C., Huang, S. J., Hu, S.Y., Liu, W. T., Lin, W. Z., Wang, W. L., Kawakami, K. and Wu, J. L.*, "IMP2 Overexpression Induces Steatosis and ER Stress in the Liver of Transgenic Zebrafish.," The 23th Joint Annual Conference of Biomedical Sciences. P281., Mar, 2008.
155. Gong, H. Y., Lin, M. Y., Huang, H. P., Lin, G. H., Lin, J. F., Amali, A. A., Huang, S. J., Lee, H. M., Lin, W. Z., Liu, W., Hu, S. Y., Wang, W. L., Kawakami, K. and Wu, J. L.*, "Transgenic zebrafish as an animal model to study nonalcoholic fatty liver disease (NAFLD).," Annual Meeting of the Fisheries Society of Taiwan., Dec, 2007.
156. Lin, M. Y., Gong, H. Y. and Wu, J. L.*, "IGF2BP2 Induces Fatty Liver in Transgenic Zebrafish.," Annual Meeting of the Fisheries Society of Taiwan., Dec, 2007.
157. Gong, H. Y., Lin, M.Y., Lin, C.J.F., Lin, G.H., Huang, S.J., Lin, W.Z., Amali, A.A., Hu, S.Y., Kawakami, K. and Wu, J.L.*, "Functional roles of IMP1/CRD-BP and IGF-II in HCC formation of transgenic zebrafish with ubiquitous or liver-specific overexpression.," The 22th Joint Annual Conference of Biomedical Sciences., Mar, 2007.
Book and Report
1. 黃章文、徐德華、龔紘毅, "媒合題目: 水產優良品種改良暨遺傳管理之精準選育," 「大學鏈結產業商機∞系列—2020海洋科技成果論壇及產學媒合會、人才面談會」。2020年6月23日。臺灣海洋大學。, 研發成果產學媒合會談, Jun, 2020.
2. 龔紘毅, "媒合題目: 養殖魚類(觀賞魚及食用魚)之精準育種," 「大學鏈結產業商機∞系列—2020海洋科技成果論壇及產學媒合會、人才面談會」。2020年6月23日。臺灣海洋大學。, 研發成果產學媒合會談, Jun, 2020.
3. 徐德華、龔紘毅、黃章文、李宏泰, "分子標記在水產品認證的應用," 漁業推廣月刊 403期: 6-9頁。, Apr, 2020.
4. 黃章文、徐德華、龔紘毅, "吳郭魚(台灣鯛)基因體分子標記輔助選育技術之研發與應用," 前瞻基因體學技術於農業領域之研發應用與展望。332頁。第110~125頁。國立臺灣大學動植物農業產業創新領域教學推動中心。, Feb, 2020.
5. 龔紘毅, "以肌肉專一性表現系統建立快速生長基因轉殖吳郭魚作為生物反應器(Establishment of fast-growing transgenic tilapia as a bioreactor by muscle-specific expression system)," 2016台北國際發明暨技術交易展。9/29-10/1。科技館。, Sep, 2016.
6. 龔紘毅, "粉紅螢光神仙魚- 新穎肌肉增強子表現系統在海洋生技及生醫產業之應用," 邁向頂尖大學計畫成果系列展3- 生技醫藥 關鍵未來成果手冊。p34-35。, May, 2015.
7. Thomas T. Chen*, Chun-Mean Lin, Maria J. Chen, Jay H. Lo, Pinwen Peter Chiou, Hong-Yi Gong, Jen-Leih Wu, Mark Hung-Chih Chen, and Charlie Yarish, "Chapter 13. Transgenic Technology in Marine Organisms," Springer Handbook of Marine Biotechnology. 2015, pp387-412. Kim, Se-Kwon (Ed.). 1560p., ISBN: 978-3-642-53970-1 (Print) 978-3-642-53971-8 (Online), Jan, 2015.
Marine organisms into which a foreign gene or noncoding DNA fragment is artificially introduced and stably integrated in their genomes are termed transgenic marine organisms. Since the first report in 1985, a wide range of transgenic fish and marine bivalve mollusks have been produced by microinjecting or electroporating homologous or heterologous transgenes into newly fertilized or unfertilized eggs and sperm. In the past few years, rapid advances of gene transfer technology has resulted in production of many genetically modified organisms (GMO) such as fish, crustacean, microalgae, macroalgae and sea urchin. These GMOs are valuable in assisting the advances of basic research as well as biotechnological applications. In this chapter, the principle of producing transgenic marine organisms and the application of the technology to produce genetic modified marine organisms in the hope of improving the quality of human life as well as the earth environments will be critically reviewed.
8. 龔紘毅*,陳鳴泉,吳金洌,黃士晉, "新穎肌肉增強子序列及其應用," 2014台北國際發明暨技術交易展榮獲發明競賽生物技術類首獎- 鉑金獎。台北國際發明暨技術交易展 2012-2014鉑金獎(Platinum Awards)專刊,p44。, Oct, 2014.
「新穎肌肉增強子序列及其應用」技術說明:
本發明係關於一種來自斑馬魚肌肉型肌酸激酶ckmb基因之新穎肌肉增強子序列及其應用。本專利技術之肌肉專一性表現單元,包含一個肌肉專一性啟動子及一至數個可提升啟動子活性之強烈肌肉增強子,可驅動螢光蛋白或功能性蛋白基因在斑馬魚、神仙魚及尼羅吳郭魚之肌肉強烈表現。應用此新穎魚類肌肉專一性表現單元成功開發出全世界第一個表現臺灣軸孔珊瑚紅色螢光蛋白之粉紅神仙魚品系。並可進一步應用於發展基因轉殖吳郭魚做為生物反應器,以肌肉做為表現組織生產水產養殖產業及醫藥用之重要重組蛋白或生物材料。此專利技術之應用可包含新穎中大型螢光觀賞魚開發、促進生長或抗病之功能性飼料添加物開發、開發吳郭魚大鱗片做為人工生物眼角膜之生物材料,及以肌肉表現病原蛋白抗原之DNA疫苗開發。
A Novel Muscle Enhancer Sequence and Application Thereof:
The invention patent relates to a novel muscle enhancer sequence identified from zebrafish muscle-type creatine kinase gene ckmb and its applications. The patented technology “muscle-specific expression element” composed of a muscle-specific promoter and one to several copies of strong muscle enhancer to enhance promoter activity, can be used to strongly express fluorescent protein or functional protein genes in the muscle of zebrafish, angelfish (Pterophyllum scalare var.) and Nile tilapia. This technology was successfully applied to establish the world first transgenic pink angelfish line expressing Taiwan Acropora coral red fluorescent protein. Furthermore, it can be applied to establish transgenic tilapia as bioreactor by using skeletal muscle as expression tissue to generate critical recombinant proteins or biomaterials for aquaculture or biomedical industry. Furthermore, it can be applied to establish transgenic tilapia as bioreactor by using skeletal muscle as expression tissue to generate critical recombinant proteins or biomaterials for aquaculture or biomedical industry. Applications of this patented muscle enhancer expression technology can include establishment of novel middle- or large-sized fluorescent ornamental fish, development of functional feed supplement to promote growth or disease-resistance, development of large scales (diameter >2cm) of tilapia as biomaterials for artificial bio-cornea and DNA vaccine development by muscle-expressing protein antigen of pathogen.
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Recipients of the Platinum Awards, the highest honor for inventors in Asia, were announced on the afternoon of 20th September. The awards were presented by Ms Wang Mei-hua, Director General of the Intellectual Property Office. A total of 960 inventions from 15 countries participated in the invention competition held during the 10th Taipei International Invention Show & Technomart. Mr. Mark Sheahan, President of the Institute of Patentees and Inventors of Great Britain and a judge for this contest, quoted that the Taipei Invention Show deserves the most praise amongst the many invention shows he had been to worldwide. He said that many mature inventions on show were absolutely stunning, displaying the creativity as well as practicality of Taiwanese inventions. He also commented on the huge commercial potential of several inventions.

Vice President Wu Den-Yih, who addressed the audience during the opening ceremony, praised Taiwan’s inventors for their continuous effort in innovation, research, and development. He also mentioned the 3 elements needed in a country’s development, which are productivity, imagination, and ability to self manufacture. Among the 3 elements, imagination is the ability to invent and innovate, and the 2,000 plus pieces of new inventions and technology on show are a testament to Taiwan’s ability to invent and innovate. Taiwan’s focus on both quality and quantity in inventions can be seen from the yearly National Invention Award and the Invention Competition segment of the Invention show.

Among the 960 inventions sorted into 15 categories, 1 from each category is selected to receive the Platinum award, with an additional 153 gold medals, 153 silver medals, and 201 bronze medals. Platinum award winners include the “Bone Conduction Hearing Aid” by Chu Ka Industrial Co. Ltd., which benefits hearing aid users tremendously by using audio shock waves to transmit audio to the ear rather than the traditional ear hook method. National Chin-Yi University of Technology showcased the “The Surface Coating Method of Solar Panel” which transforms dull solar panels into aesthetic art pieces to which can be used as installation art in public places. “A Novel Muscle Enhancer Sequence and Applications Thereof” patent by the National Taiwan Ocean University is used mainly to drive the muscle performance of fluorescent protein genes in Zebra fish, Angelfish and Tilapia, and has also successfully created the world’s first transgenic pink Angelfish. The “Radiotherapy system adapted to monitor a target location in real time” by Mackay Memorial Hospital can detect the position of a tumor accurately, reducing the discomfort of patients during radiotherapy.
9. 龔紘毅, "新穎魚類肌肉專一性增強子/啟動子在水產養殖應用之潛力," 國立臺灣海洋大學海洋中心電子報第六期, Jan, 2014.
水產養殖學系龔紘毅老師在海洋中心及農委會與國科會產學合作計畫支援下,自斑馬魚找出硬骨魚類特有之肌肉專一性肌肉型肌酸激酶基因(CKMb)調控序列(包含一新穎強烈肌肉增強子),可強烈驅動斑馬魚骨骼肌及心肌之螢光蛋白基因表現。於2012年4月將「新穎肌肉專一性表現單元應用於建立基因轉殖螢光觀賞魚」技術轉移給產學合作之觀賞魚廠商芝林公司。已成功開發以顯微注射法建立肌肉專一性表現軸孔珊瑚紅色螢光蛋白之全世界第一個基因轉殖粉紅神仙魚(Pterophyllum scalare)及粉紅金神仙魚品系,並技轉給芝林公司。此「新穎肌肉增強子序列及其應用」已於2013年7月取得中華民國發明專利(第I402343號)。龔紘毅老師利用此技術,發現一新穎吳郭魚免疫調節分泌胜肽OmGRN-41基因,以肌肉適量表現可顯著活化先天免疫相關基因而增進魚類之抗病力。並可利用大腸桿菌大量生產OmGRN-41可溶性免疫調節胜肽。此「吳郭魚先天免疫調節胜肽OmGRN-41以增強魚類抗病存活率技術」已於2012年12月技轉給全興國際水產公司,以期進一步開發做為飼料功能性添加物。因此利用此新穎魚類肌肉專一性增強子/啟動子可以肌肉做為旁分泌/自分泌組織,來研究參與魚類抗病或生長功能性基因之作用機制。亦可應用於建立開發新的基因轉殖觀賞魚品系,及發展以魚類肌肉做為生物反應器(bioreactor)來生產水產養殖產業或醫藥用之重要蛋白質。
10. 林峰右、吳育甄、胡益順、黃致中、龔紘毅、葉信利, "水產種苗研究團隊-龍膽石斑遺傳育種之研究," 水產試驗所2013年年報。p41。, Dec, 2013.
11. 龔紘毅, "新穎肌肉增強子與啟動子組合表現單元增進觀賞魚生長及抗病之應用," 2013台北國際發明暨技術交易展。農業館技術專刊。p75-76。, Sep, 2013.
技術說明: 本研究自斑馬魚肌肉專一性CKMb 基因intron 1內發現一335 bp增強子(EnIn1),將其置於CKMb 2.4 kb啟動子/增強子前可加強其在肌肉之表現活性。將四個EnIn1增強子置於CKMb 251 bp近端啟動子前可大幅增強其啟動子活性。以肌肉專一性CKMb增強子/啟動子表現台灣軸孔珊瑚紅色螢光蛋白基因,已建立世界第一個基因轉殖粉紅神仙魚品系。利用此新穎魚類肌肉專一性CKMb增強子與啟動子組合表現單元建立基因轉殖斑馬魚模式,以肌肉做為旁分泌/自分泌組織表現參與魚類生長或抗病之功能性基因以增進觀賞魚生長或抗病力。
12. 林峰右、吳育甄、胡益順、黃致中、龔紘毅、葉信利, "水產種苗研究團隊-龍膽石斑遺傳育種之研究," 水產試驗所2013年年報。41頁。, Jan, 2013.
13. Gong, H. Y.*, Wu, S. H., Lin, H. J., Chin, H. Y., "表現及生產新穎吳郭魚先天免疫調節胜肽 OmGRN-41 以增強魚類抗病原菌存活力(Expression and production of a novel tilapia innate immune modulating peptide OmGRN-41 to enhance survivability against bacterial pathogens.)," 2012台北國際發明暨技術交易展。國科會科技創新館。(2012 Taipei International Invention Show and Technomart. Innovative Technologies of National Science Council. Sep.20~23. Taipei.), Sep, 2012.
14. 龔紘毅, "新穎肌肉專一性啟動子/增強子(promoter/enhancer) 應用在魚類肌肉做為生物反應器(bioreactor)生產重組蛋白," 轉譯農學產業智財專利管理與產業創新論壇。100年度教育部顧問室「轉譯醫學及農學人才培育先導型計畫」。, Jan, 2012.
15. 龔紘毅*, "新穎肌肉增強子序列及其應用。," 2011年生醫暨農業生技成果媒合發表會。技術手冊P111。100年9月7日。中央研究院。, Sep, 2011.
16. 耿全福、龔紘毅、陳志毅、廖欽峰、黃鵬鵬、吳金洌。, "電破法魚類轉殖技術之研發。," 生物技術在水產養殖上應用研討會論文集。29 - 47 頁。, Jan, 1994.
17. 龔紘毅編, "七十七年度中山大學南海岸生態研究隊報告書-小琉球海岸生態研究。," 國立中山大學海洋資源學系,高雄,台灣。, Oct, 1988.
龔紘毅擔任七十七年度中山大學南海岸生態研究隊總幹事。
18. 陳鳴泉&龔紘毅, "小琉球潮間帶棘皮動物的分布及生態研究。," 七十七年度中山大學南海岸生態研究隊報告書-小琉球海岸生態研究。14-46頁。國立中山大學海洋資源學系,高雄,台灣。, Oct, 1988.
Patent
1. 龔紘毅、陳澤君、林仲彥, "抗病與成長性狀功能性基因標記資料庫," 兩年非專屬授權技術轉移“基育生物科技股份有限公司”。2020/06/29~2022/06/28。, Jul, 2020.
技術來源:
本項技術係乙方執行行政院農業委員會漁業署補助之研究計畫「建構台灣鯛基因體育種分析整合平台以應用於抗病台灣鯛分子標誌輔助選育(計畫編號:109農科-12.3.1-科-a2)」之研發成果。惟本項技術乙方利用甲方資源進行研究所衍生之研發成果,其智慧財產權為甲方(國立臺灣海洋大學)所有。

技術內容:
利用分子標記輔助種原庫建立遺傳育種資訊並開發抗逆境性狀功能性基因標記選育策略為提升重要經濟魚種改良之重要關鍵。本技術為利用高通量次世代定序平台從臺灣鯛基因體(genome)與轉錄體(transcriptome)基因庫中搜尋參與抗病與成長功能且具差異性表現之功能性基因群資訊庫,進一步從中開發出具有抗病性狀遺傳變異相關之單一核苷酸多型性(single nucleotide polymorphism,SNPs)基因標記,利用此基因標記資料庫所開發而成之SNP微陣列晶片為提供「臺灣鯛抗病與成長品系」種原親本及其子代基因體選育之核心關鍵技術。抗病與成長性狀功能性基因標記資料庫平台技術具有優質種苗抗病性狀分子調控機制之基因體選育(genomic selection,GS)與市售商用水產品之品質檢定與驗證之優勢,透過系譜追蹤系統與基因標記鑑定平台之分子育種資訊,可有效培育抗病與成長或其它性狀(如耐逆境等)性能兼具,提供種苗繁養殖業者建立符合國內外消費市場與商業價值指標之優質臺灣鯛品牌。
2. 龔紘毅、陳鳴泉、吳金洌、黃士晉 (Inventors: Hong-Yi Gong, Ming-Chuyan Chen, Jen-Leih Wu, Shih-Chin Huang), "肌肉增強子序列及其應用 (Muscle enhancer sequences and its application)," 中國發明專利 (証書號 第1369854號)。專利號:ZL 201110221498.0。授權公告日: 2014/03/26。專利期間:2014/03/26~2031/08/02, 申請人:龔紘毅。專利權轉讓于台灣海洋大學。, Mar, 2014.
本发明系关于一种来自斑马鱼肌肉型肌酸激酶b(ckmb)基因之新颖肌肉增强子及其应用。本发明之增强子可提升启动子活性,可用于制备转殖基因动物及生物反应器之应用。
The present invention relates to a type of muscle enhancer from zebrafish muscle creatine kinase b (ckmb) gene and its application. Enhancer of the invention can enhance promoter activity and can be used for the preparation of transgenic animals and used in the bioreactor.
3. 龔紘毅, "顯微注射法建立基因轉殖螢光神仙魚品系及多轉基因插入位點鑑定技術," 技術移轉"芝林生物科技股份有限公司"。, 五年非專屬授權。2014年2月11日。, Feb, 2014.
4. 龔紘毅、陳鳴泉、吳金洌、黃士晉, "新穎肌肉增強子序列及其應用(A NOVEL MUSCLE ENHANCER SEQUENCE AND APPLICATIONS THEREOF)," 中華民國發明專利。專利證號:發明第 I 402343號。申請日: 2011/1/20。專利期間: 2013/7/21~ 2031/1/19。申請人:國立台灣海洋大學 NATIONAL TAIWAN OCEAN UNIVERSITY。, Jul, 2013.
本發明係關於一種來自斑馬魚肌肉型肌酸激酶b(ckmb)基因之新穎肌肉增強子及其應用。本發明之增強子可提升啟動子活性,可用於製備轉殖基因動物及生物反應器之應用。
5. 賴弘基、吳金洌、耿全福、龔紘毅、林建成、陳永貴、周正鴻, "層狀結構," 中華民國新型專利(核准)第M453583號, 專利權始日:2013/05/21~ 專利權止日:2022/12/25。申請人:柏登生醫股份有限公司 BODY ORGAN BIOMEDICAL CORP., May, 2013.
一種層狀結構包括:複數混合材料層。此些混合材料層依序層疊。其中,各混合材料層包括相互混合之至少一種人類膠原纖維及至少一種無機質。
6. 龔紘毅, "吳郭魚先天免疫調節胜肽OmGRN-41以增強魚類抗病存活率技術," 先期技轉給"全興國際水產股份有限公司", 五年非專屬授權 (2012/12/26 ~2017/12/25), Dec, 2012.
本項技術係執行國科會補助專題研究計畫「建立生長及變態相關 基因分子標誌以進行石斑魚優良品種選育(計畫編號: 99-2324-B-019-004-MY2)」之研究成果。 本研究室從吳郭魚發現含有 41 個氨基酸之新穎分泌型短胜肽,將其命名為 OmGRN-41。利用魚類肌肉專一性啟動子建立基因轉殖斑馬魚表現 OmGRN-41 分泌 胜肽。發現 OmGRN-41 具有強烈活化及調節先天性免疫如 IL-8、IL-1β、TNFα、C3b 等基因之作用。以創傷弧菌或鏈球菌感染野生型斑馬魚與表現 OmGRN-41 之基因轉 殖斑馬魚稚魚相比,發現表現 OmGRN-41 其可大幅提高其感染後存活率。顯示 OmGRN-41 胜肽為可經由活化先天免疫相關基因來增加魚類抗病原菌能力之新穎免 疫調節胜肽。將 OmGRN-41 之 41 個氨基酸成熟胜肽 cDNA 構建至大腸桿菌表現載 體 pGS-21a 得到 pGST-OmGRN-41-(His)6 表現載體。我們已建立以大腸桿菌表現 OmGRN-41 胜肽可溶性重組蛋白系統,可大量表現及純化此新穎免疫調節活性胜 肽。可將此 OmGRN-41 cDNA 轉接到不同的原核及真核表現載體來大量表現其重組 蛋白,以應用於重要水產養殖魚種如吳郭魚、石斑魚等,做為提升免疫及抗病之飼 料功能性添加物。
7. 龔紘毅, "新穎肌肉專一性表現單元應用於建立基因轉殖螢光觀賞魚," 技轉移轉"芝林企業有限公司", 五年非專屬授權。2012年4月16日。, Apr, 2012.
技術名稱:新穎魚類肌肉專一性表現單元應用於建立基因轉殖螢光觀賞魚。「新穎肌肉專一性表現單元(expression cassette)」包含斑馬魚(Danio rerio)及莫三比克吳郭魚(Oreochromis mossambicus)之新穎肌肉型肌酸激酶CKMb基因啟動子(promoter)及增強子(enhancer)之肌肉專一性表現單元,可連接螢光蛋白基因cDNA在魚類肌肉強烈表現螢光蛋白以應用於建立基因轉殖螢光觀賞魚。
技術來源:執行行政院農業委員會補助專題研究計畫「開發肌肉強烈表現台灣珊瑚新型螢光蛋白之基因轉殖螢光觀賞魚(計畫編號:98農科-1.2.1-科-ap, 99農科-1.2.3-科-aF)」之研究成果。
Other
1. 游睿軒(Jui-Hsuan Yu), "利用CRISPR/Cas9基因體編輯建立肌肉抑制素b突變尼羅吳郭魚品系以促進其肌肉生長 (Establishment of myostatin b mutated Nile tilapia strain by CRISPR/Cas9 genome editing to enhance muscle growth)," 國立臺灣海洋大學水產養殖系碩士論文。2020年2月。, 指導教授:龔紘毅博士。 (Advisor: Dr. Hong-Yi Gong), Feb, 2020.
2. 林詩庭 (Shi-Ting Lin), "建立 fat-1基因轉殖魚以增進omega-3多元不飽和脂肪酸合成及其抗發炎分子機制研究 (Establishment of fat-1 transgenic fish to enhance omega-3 PUFAs synthesis and to study their molecular mechanism of anti-inflammation)," 國立臺灣海洋大學水產養殖學系碩士論文, 指導教授:龔紘毅博士 (Advisor: Dr. Hong-Yi Gong), Aug, 2019.
3. 朱慧君(Wai-Kwan Chu), "以 CRISPR/Cas9 基因編輯技術建立 dead end (dnd1) 標靶突變斑馬魚及淡水神仙魚之不孕控制技術(Targeted mutagenesis of dead end (dnd1) gene for infertility control of zebrafish and angelfish by CRISPR/Cas9 genome editing)," 國立臺灣海洋大學水產養殖學系碩士論文, 指導教授:龔紘毅博士&張清風博士 (Advisor: Dr. Hong-Yi Gong & Ching-Fong Chang), Jul, 2019.
4. 吳聖韓 (Sheng-Han Wu), "莫三比克吳郭魚短型顆粒蛋白前體PGRN1基因在抗弧菌之分子機制研究(Study on molecular mechanism of short-form PGRN1 gene from Mozambique tilapia in defense against Vibrio species)," 國立臺灣海洋大學水產養殖學系博士論文, 指導教授:龔紘毅博士 (Advisor: Dr. Hong-Yi Gong), Jul, 2019.
5. 馬家桓 (Chia-Huan Ma), "虎斑烏賊繁養殖技術及微衛星標記的開發(Development of culture techniques and microsatellite markers of pharaoph cuttlefish (Sepia pharaonis).)," 國立台灣海洋大學水產養殖系碩士論文(Master Thesis, Department of Aquaculture, National Taiwan Ocean University), 指導老師:徐德華 & 龔紘毅博士 (Advisor: Dr. Te-Hua Hsu & Hong-Yi Gong), Jul, 2018.
6. 范紅日(Pham Hong Nhat), "Hepcidin家族基因相關微衛星具有潛力作為抗病吳郭魚選育之分子標誌 (Hepcidin family genes associated microsatellites exert potentially molecular markers for selective breeding of disease-resistant tilapia)," 國立台灣海洋大學水產養殖系碩士論文(Master Thesis, Department of Aquaculture, National Taiwan Ocean University) (in English), 指導教授:龔紘毅博士 (Advisor: Dr. Hong-Yi Gong), Jan, 2018.
7. 蔡宜庭 (Yi-Ting Tsai), "肌肉抑制素a與b在單基因及雙基因剔除斑馬魚之角色研究 (Study on roles of Myostatin a and b in single and double gene knockout zebrafish)," 國立台灣海洋大學水產養殖系碩士論文(Master Thesis, Department of Aquaculture, National Taiwan Ocean University), 指導教授:龔紘毅博士 (Advisor: Dr. Hong-Yi Gong), Jul, 2017.
8. 何佳蕙 (Chia-Hui Ho), "尼羅吳郭魚三個肝臟抗菌肽HAMP基因在肝臟、脾臟、頭腎及鰓被強烈活化以對抗致病性海豚鏈球菌 (Three hepatic antimicrobial peptide HAMP genes were strongly activated in liver, spleen, head kidney and gill in Nile tilapia to defend against virulent Streptococcus iniae)," 國立台灣海洋大學水產養殖研究所碩士論文(Master Thesis, Department of Aquaculture, National Taiwan Ocean University), 指導教授:龔紘毅博士 (Advisor: Dr. Hong-Yi Gong), Jul, 2016.
9. 林文福 (Wen-Fu Lin), "尼羅吳郭魚短型顆粒蛋白前體 2 (PGRN2) 調節先天性免疫相關基因抵抗鏈球菌之研究 (Study on Short-form Progranulin 2 (PGRN2) of Nile Tilapia Modulates Innate Immune-related Genes against Streptococcus)," 國立台灣海洋大學水產養殖研究所碩士論文(Master Thesis, Department of Aquaculture, National Taiwan Ocean University), 指導教授:龔紘毅博士 (Advisor: Dr. Hong-Yi Gong), Jul, 2016.
10. 魏梓傑 (Zih-Jie Wei), "以魚類生殖細胞專一性Piwi1啟動子建立基因轉殖魚誘導式不孕技術 (Establishment of inducible infertility technology for transgenic fish by teleost germline-specific Piwi1 promoter)," 國立台灣海洋大學水產養殖研究所碩士論文(Master Thesis, Department of Aquaculture, National Taiwan Ocean University), 指導教授:龔紘毅博士 (Advisor: Dr. Hong-Yi Gong), Jan, 2016.
11. 盧佳彣 (Chia-Wen Lu), "新穎甲殼類顆粒蛋白前體基因在白蝦之表現及功能研究 (Expression and functional study of a novel crustacean progranulin gene in white shrimp)," 國立臺灣海洋大學水產養殖研究所碩士論文(Master Thesis, Department of Aquaculture, National Taiwan Ocean University), 指導教授:龔紘毅博士 (Advisor: Dr. Hong-Yi Gong), Jul, 2015.
12. 曾柏鈞 (Po-Chun Tseng), "龍膽石斑骨骼肌生成相關基因之微衛星及缺失DNA標誌可應用於生長之分子標誌輔助育種 (Microsatellites and deletion markers in skeletal myogenesis related genes of giant grouper can be applied in marker assisted selection of growth)," 國立台灣海洋大學水產養殖研究所碩士論文(Master Thesis, Department of Aquaculture, National Taiwan Ocean University), 指導教授:龔紘毅博士 (Advisor: Dr. Hong-Yi Gong), Jul, 2015.
13. 王韵倫 (Yun-Lung Wang), "Akirin2在斑馬魚肝臟成長及病理發生上之角色與機制研究 (Roles and molecular mechanisms of akirin2 in liver growth and pathogenesis of zebrafish)," 國立台灣海洋大學水產養殖研究所碩士論文(Master Thesis, Department of Aquaculture, National Taiwan Ocean University), 指導教授:龔紘毅博士 (Advisor: Dr. Hong-Yi Gong), Jun, 2015.
14. 郭怡萱, "白蝦脂多醣與葡聚醣結合蛋白與海藻多醣辨識與結合並啟動先天性免疫系統 (Recognition and binding of lipopolysaccharide and β-glucan binding protein with seaweed polysaccharides elicit the innate immunity of white shrimp Litopeneaus vannamei)," 國立台灣海洋大學水產養殖研究所碩士論文 (Master Thesis, Department of Aquaculture, National Taiwan Ocean University), 指導教授:龔紘毅博士&陳建初博士, Jan, 2015.
15. 張瑜軒, "白蝦粒線體錳型超氧歧化酶之功能分析 (The function of mitochondrial manganese superoxide dismutase in white shrimp Litopenaeus vannamei)," 國立台灣海洋大學水產養殖研究所碩士論文 (Master Thesis, Department of Aquaculture, National Taiwan Ocean University), 指導教授:龔紘毅博士&陳建初博士, Jan, 2015.
16. 鄭頌雅 (Clare Siing Nga Tang), "吳郭魚新穎短型顆粒蛋白前體調控斑馬魚先天性免疫 (Regulation of Innate Immunity by Tilapia Novel Short Form Progranulin in Zebrafish)," 國立台灣海洋大學水產養殖研究所碩士論文 (Master Thesis, Department of Aquaculture, National Taiwan Ocean University)(in English), 指導教授: 龔紘毅 博士 (Advisor: Dr. Hong-Yi Gong), Jul, 2013.
17. 戴澤宇 (Tse-Yu Tai), "以龍膽石斑轉錄體發展標誌輔助育種之生長相關第一型微衛星DNA標誌 (Development of Growth-Related Type I Microsatellite DNA Markers from the Transcriptome of Giant Grouper for Marker-Assisted Selection)," 國立台灣海洋大學水產養殖研究所碩士論文 (Master Thesis, Department of Aquaculture, National Taiwan Ocean University), 指導教授: 龔紘毅 博士 (Advisor: Dr. Hong-Yi Gong), Jul, 2012.
18. 陳韋燕 (Hui-Yen Chin), "肌肉專一性表現龍膽石斑生長激素在基因轉殖斑馬魚之功能性研究 (Functional Studies of Transgenic Zebrafish With Giant Grouper's Growth Hormone Specific Expression In Muscle)," 國立台灣海洋大學水產養殖研究所碩士論文 (Master Thesis, Department of Aquaculture, National Taiwan Ocean University) (in English), 指導教授: 龔紘毅 博士 (Advisor: Dr. Hong-Yi Gong), Jul, 2012.
19. 吳聖韓 (Sheng-Han Wu), "吳郭魚 Granulins 調控魚類內生性免疫之分子機制研究及其應用 (Molecular mechanism and application of tilapia Granulins in the regulation of innate immunity of teleost)," 國立台灣海洋大學水產養殖研究所碩士論文 (Master Thesis, Department of Aquaculture, National Taiwan Ocean University), 指導教授: 龔紘毅 博士 (Advisor: Dr. Hong-Yi Gong), Jul, 2012.
20. 陳智琦 (Chih-Chi Chen), "Akirin基因家族調節斑馬魚肌肉肥大之功能性研究 (Functional study of Akirin gene family on the regulation of muscle hypertrophy in zebrafish)," 國立台灣海洋大學水產養殖研究所碩士論文 (Master Thesis, Department of Aquaculture, National Taiwan Ocean University), 指導教授: 龔紘毅 博士 (Advisor: Dr. Hong-Yi Gong), Jan, 2012.
21. 黃千綺 (Chian-Chi Huang), "IGF-IR訊息路徑參與在肝臟專一性表現IMP2基因轉殖斑馬魚肝臟內膽道癌化過程之早期階段誘導膽道細胞增生 (IGF-IR Signaling Participates in Cholangiocyte Proliferation during Early Stage of Intrahepatic Cholangiocarcinoma in Liver-Specific IMP2 Transgenic Zebrafish)," 國立台灣海洋大學水產養殖研究所碩士論文 (Master Thesis, Department of Aquaculture, National Taiwan Ocean University), 指導教授: 龔紘毅 博士& 吳金洌 博士 (Advisor: Dr. Hong-Yi Gong & Dr. Jen-Leih Wu), Jun, 2011.
22. 林宏傑 (Hong-Jie Lin), "莫三比克吳郭魚Progranulin家族基因之表現及其在基因轉殖斑馬魚調控肌肉生成和先天免疫基因之功能研究 (Tilapia (Oreochromis mossambicus) Progranulin family genes expression and functional study on the regulation of myogenesis and innate immunity genes in transgenic zebrafish)," 國立台灣海洋大學水產養殖研究所碩士論文 (Master Thesis, Department of Aquaculture, National Taiwan Ocean University), 指導教授: 龔紘毅 博士 (Advisor: Dr. Hong-Yi Gong), Jan, 2011.
23. 黃士晉 (Shih-Chin Huang), "建立肌肉專一性啟動子表現新穎台灣軸孔珊瑚螢光蛋白之基因轉殖螢光魚 (Establishment of transgenic fluorescent fish expressing novel Taiwan Acropora coral fluorescent proteins by teleost muscle-specific promoter)," 國立台灣海洋大學水產養殖研究所碩士論文 (Master Thesis, Department of Aquaculture, National Taiwan Ocean University), 指導教授: 龔紘毅 博士 (Advisor: Dr. Hong-Yi Gong), Jul, 2010.