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Name: 許濤
Department: 國立海洋大學 / 生科系大學部課程 [elearning courses]
Research: 環境毒物學、免疫學、生物技術、藻類分子生物學、應用免疫學
Office: 綜合二館208室
Self-Introduction

許濤老師 研究室介紹

 

主要研究領域：水生環境生物學、分子毒理學

 

相關研究領域：環境毒物學、免疫學、生物技術、藻類分子生物學、應用免疫學

 

使用生物材料：斑馬魚、小球單胞綠藻、大腸桿菌

 

研究計畫簡介：

1.  熱休克蛋白70於斑馬魚發育過程中之自發性表現、其組織特異性分佈與轉譯調控：熱休克蛋白70 為細胞內將變性蛋白質摺疊回正常構造之分子監護者(molecular chaperone)。自發性表現之熱休克蛋白70包括等電點分別為5.5與5.7之Hsp70與Hsc70，但Hsc70於受精84 小時候(84 hpf)斑馬魚中之自發性表現量為Hsp70之10倍，然而Hsc70對熱休克之敏感度較Hsp70為差。當Hsc70尚未出現在36 hpf斑馬魚胚胎中時，全覆式原位雜交顯示其mRNA已廣泛分佈在各組織中，反應出此Hsc70之自發性大量表現係於轉譯層次調控。由於Hsp70 mRNA有一較Hsc70 mRNA為長之5’端非轉譯區域，且Hsp70 mRNA於兔子紅血球抽取液中之轉譯效率明顯優於Hsc70 mRNA，因此5’端非轉譯區域在胚胎發育時期之修飾狀態與此區域結合蛋白之含量可能為調控Hsc70 mRNA轉譯能力之關鍵。

 

2.  化學物質引起之氧化壓力抑制斑馬魚胚胎DNA錯誤配對修補因子基因表現之機制：真核生物中單一錯誤配對與配對不完全之小型寡核苷酸環由MSH2-MSH6蛋白複合體進行辨識，而MSH6為主要之辨識蛋白。本實驗室發現具致癌性之氯化鎘或殺草劑巴拉刈(paraquat)於非致死濃度下處理斑馬魚胚胎4至9小時可抑制MSH2/MSH6基因於 mRNA與蛋白質層及之表現，但不干擾β-actin基因活性。基於巴拉刈為一已知之superoxide radical(O2-)誘發劑與鎘對SOD之抑制效果，推論鎘應先引起具組織傷害性之氧化壓力，再以活性氧分子為主要迅號傳遞者調降斑馬魚胚胎中MSH基因表現，最終導致癌症之產生。

 

實驗室常用技術：

全覆式原位雜交、即時定量RT-PCR、分子選殖、基因轉殖、蛋白質純化、分子演化分析

 

未來產業連結：

學術研究、環境生物科技、醫學分子檢驗

Education
1. 美國密西根大學毒理學博士 (Doctor)
Publication
Paper
1. Yung Chang, Wei-Yuan Lee, Yu-Jie Lin, Todd Hsu*, "Mercury (II) impairs nucleotide excision repair (NER) in zebrafish (Danio rerio) embryos by targeting primarily at the stage of DNA incision," Aquatic Toxicology, Sep, 2017.
2. Li-Bin Ling, Yung Chang, Chia-Wei Liu, Po-Ling Lai, Todd Hsu*, "Oxidative stress intensity-related effects of cadmium (Cd) andparaquat (PQ) on UV-damaged-DNA binding and excision repair activities in zebrafish (Danio rerio) embryos. Chemosphere 167:10-18, 2017.," , 2017.
3. Yung-Chi Shen, Todd Hsu, Li-Bin Ling, Wen-Chian You, Chia-Wei Liu, "Identification of low-moleculr-weight vitellogenin 1 (Vg1)-like proteins as nucleotide excision repair (NER) factors in developing zebrafish (Danio rerio) using a transcription-based DNA repair assay. Fish Physiol Biochem 43:663-676., 2017.," , 2017.
4. Tsung-Nan Ho, Shin-Tsung Sung, Kuan-Ming Huan, Todd Hsu*, "Comparative effects of mercury (ll) and cadmium on Muts homolog 6(Msh6)-mediate DNA mismatch binding activities in zebrafish (Danio rerio) embryo. J. Biochem. Mol. Toxicol. 29:513-520(SCI)," , 2015.
5. Cheng-Ta Yang, Yi-Cheng Wu, Ming-Mo Hou, Chia-Hsun Hsieh, Jia-Juan Hsieh, Hsin-Yi Cheng, Todd Hsu, John Wen-Cheng Chang, "Cytoplasmic and nuclear parathyroid hormone-related proteins are opposing prognostic factors in patients with non-small-cell lung cancer who have undergone curative resection. Jpn. J. Clin. Oncol. 45(3): 267-273. (SCI)," , 2015.
6. Todd Hsu∗, Kuan-Ming Huang, Huei-Ting Tsai, Shih-Tsung Sung, Tsung-Nan Ho, "Cadmium(Cd)-induced oxidative stress down-regulates the gene expression of DNA mismatch recognition proteins MutS homolog 2 (MSH2) and MSH6 in zebrafish (Danio rerio) embryos. Aquat. Toxicol. 126:9-16. (SCI)," , 2013.
7. Hou, Ming-Mo, Shiu-Feng Huang, Han-Pin Kuo, Cheng-Ta Yang, Ying-Huang Tsai, Chih-Teng Yu, Horng-Chyuan Lin, Chih-Hung Chen, Chih-Liang Wang, Fu-Tsai Chung, Jia-Juan Hsieh, Todd Hsu, Hsin-Yi Cheng, Li-Ying Ou, Hung-ming Wang, Yung-Chang Lin, Nai-Jen Chang,, "Erlotinib treatment in patients with advanced lung adenocarcinoma with CISH-positive and CISH-negative EGFR gene alterations. Anticancer Res. 32: 1107-1112.(SCI)," , 2012.
8. Kan-Hung Hu, Shing-Hoa Wang, Todd Hsu, Chien-Chon Chen, Tai-Yuan Lin, Huei-Ting Tsai, Hsiang-Ho Chiang, "The effect of Nitrogen-Doped ATO Nanotubes on Radical Multiplication of Buffer Media by Visible Light Photocatalysis Rather UV. J. Nanomater. 2012, Article ID 510672, 6 pages.(SCI)," , 2012.
9. Lai, Yi-Show, Feng-Ju Hsieh, Todd Hsu*, "Affinity isolation and mass spectral analysis of 1,10-phenanthroline (OP)-stimulated UV-damaged-DNA binding proteins expressed in zebrafish (Danio rerio) embryos. Fish Physiol. Biochem. 38: 1117-1129. (SCI) NSC 95-2313-B-019-003, NSC 100-2313-B-019-009," , 2012.
10. Chang, Wen-Cheng, Nien-Chih Wei, Hung-Ju Su, Jie-Len Huang, Tse-Ching Chen, Yi-Cheng Wu, Ming-Mo Hou, Chia-Hsun Hsien, Jia-Juan Hsieh, Chiao-En Wu, Todd Hsu, Tzu-Hao Wang, "Comparison of genomic signatures of non-small cell lung cancer recurrence between two microarray platforms. Anticancer Res. 32: 1259-1266.(SCI)," , 2012.
11. Yu, Chun-Kang, Kan-Hung Hu, Shing-Hoa Wang*, Todd Hsu*, Huei-Ting Tsai, Chien-Chou Chen, Shiu-Mei Liu, Tai-Yuan Lin, Chin-Hsing Chen, "Photocatalytic effect of anodic titanium oxide nanotubes on various cell culture media, Appl. Phys. (A) 102: 271-274.(SCI)," , 2011.
12. Hsieh, Chia-Hsun, John W-C Chang, Jia-Juan Hsieh, Todd Hsu, Shiu-Feng Huang, Chun-Ta Liao, Hung-Ming Wang, "Epidermal growth factor receptor mutations in patients with oral cavity cancer in a bet nut chewing-prevalent area, Head and Neck 33:1758-1764.(SCI)," , 2011.
13. Todd Hsu*, Huei-Ting Tsai, Kuan-Ming Huang, Mei-Chu Luan, Chang-Ruei Hsieh, "Sublethal levels of cadmium down-regulate the gene expression of DNA mismatch recognition protein MutS homolog 6 (MSH6) in zebrafish (Danio rerio) embryos, Chemosphere 81: 748-754.(SCI)," , 2010.
14. Hou, Ming-Mo., Jia-Juan Hsieh, Nai-Jen Chang, Hsuan-Ying Huang, Hung-Ming Wang, Cheng-Keng Chang, Todd Hsu, John Wen-Cheng Chang, "Response to sorafenib in a patient with metastatic Xp11 translocation renal cell carcinoma, Clin. Drug Investig. 30:799-804.(SCI)," , 2010.
15. Hsu, Lung-Yin., Fu-Lung Yeh, Tun-Yuan Cheng, You-Hsin Chang, Shiu-Mei Liu, Todd Hsu*, "A spontaneous high expression of heat shock cognate 70 (HSC 70) in zebrafish Danio rerio larvae arising from tissue-specific translation of preexisting mRNA, Fisheries Sci. 76: 857-864.(SCI)," , 2010.
16. Katarzyna Kazłowska, Todd Hsu, Chia-Chung Hou, Wen-Chin Yang, Guo-Jane Tsai, "Anti-inflammatory properties of phenolic compounds and crude extract from Porphyra dentate, J. Ethnopharmacol. 128: 123-130. (SCI)," , 2010.
17. Chang, Wen-Cheng., Jia-Juan Hsieh, Yung-Chi Shen, Kun-Yun Yeh, Cheng-Hsu Wang, Ying-Ying Li, Todd Hsu*, "Bisphosphonate zoledronic acid enhances the inhibitory effects of gefitinib on EGFR-mutated non-small cell lung carcinoma cells, Cancer Lett. 278:17-26. (SCI)," , 2009.
18. Chen, C-M., J.W-C. Chang, Y-C. Cheung, G. Lin, J-J. Hsieh, T. Hsu, S-F. Huang, "Computed tomography-guided core-needle biopsy specimens demonstrate epidermal growth factor receptor mutations in patients with non-small-cell lung cancer, Acta Radiologica 49:991-994. (SCI)," , 2008.
19. Liu, A-C., H-W Shih, T. Hsu, E-M Lai, "A citrate-inducible gene, encoding a putative tricarboxylate transporter, is downregulated by the organic solvent DMSO in Agrobacterium tumefaciens, J. Appl. Microbiol. 105: 1372-1383. (SCI)," , 2008.
20. Chang, J. W-C., C-L. Chou, S-F. Huang, H-M. Wang, J-J. Hsieh, T. Hsu, Y-C. Cheung, "Erlotinib response of EGFR-mutant gefitinib-resistant non-small-cell lung cancer, Lung Cancer 58:414-417. (SCI)," , 2007.
21. Yeh, Fu-Lung., Lung-Yin Hsu, Bo-An Lin, Chiao-Fu Chen, I-Chen Li, Shin-Hua Tsai and Todd Hsu*, "Cloning of zebrafish (Danio rerio) heat shock factor 2 (HSF2) and similar patterns of HSF2 and HSF1 mRNA expression in brain tissues. Biochimie 88:1983-1988.(SCI)," , 2006.
22. Lai, Yi-Show., Li-Feng Chiue and Todd Hsu*, "Low-molecular-weight vitellogenin 1-like proteins are components of a UV-damaged-DNA binding activity highly expressed in zebrafish (Danio rerio) embryos, J. Exp. Zool., 306A: 215-224. (SCI)," , 2006.
23. Chen, Fang-Ping., Todd Hsu, Chin-Hwa Hu, Wen-Der Wang, Kun-Chuang Wang, Li-Fen Teng, "Expression of estrogen receptors alfa and beta mRNA and alkaline phosphatase in the differentiation of osteoblasts from elderly postmenopausal women: comparison with osteoblasts from osteosarcoma cell line. Taiwanese J. Obstet. Gynecol. 45:307-312.," , 2006.
24. Wu Chien-Wei, Li-Jung Yin, Todd Hsu, Shann-Tzong Jiang, "Comparative Study on Antibacterial Effect of Pediocin ACCEL and Nisin Against Fluorescencestained Listeria monocytogenes BCRC 14845. J. Food Sci., 70: 75-80 (SCI)," , 2005.
25. Hsu, Todd*., Kai-Ning Chang, Yi-Show Lai, Ting-Yi Jung and Gen-I Lee, "DNA mismatch binding activities in Chlorella pyrenoidosa extracts and affinity isolation of G-T mismatch binding proteins, Plant Physiol. Biochem., 43:309-313 (SCI)," , 2005.
26. Chiou L.M, M.C. Chung, P.G. Tung, T. Hsu and J.S. Yang, "The use of egg chorion glycoprotein of Epinephelus malabaricus for egg identification, J. Fish Biol., 65:1614-1621. (SCI)," , 2004.
27. Chen, F.P., T. Hsu, C.H. Hu, W.D. Wang, K.C. Wang and L.F. Teng, "Expression of estrogen receptors alpha and beta in human osteoblasts: identification of exon-2 deletion variant of estrogen receptor beta in postmenopausal women, Chang Gung Med. J., 27: 107-115.," , 2004.
28. Yeh, Fu-Lung., Shi-Ya Wang, Lung-Ying Hsu, Dar-Yi Wang and Todd Hsu*, "Cloning of the mismatch recognition protein MSH2 from zebrafish(Danio rerio) and its developmental stage-dependent mRNA expression, Biochim. Biophys. Acta (Gene Structure and Expression)., 1680: 129-136.(SCI)," , 2004.
29. Yeh, Fu-Lung., Hwei-Ling Yan, Shi-Ya Wang, Ting-Yi Jung and Todd Hsu*, "Molecular cloning of zebrafish(Danio rerio) MutS homolog 6(MSH6) and noncoordinate expression of MSH6 gene activity and G-T mismatch binding proteins in zebrafish larvae, J. Exp. Zool., 297A: 118-129. (SCI)," , 2003.
30. Hsu, T*., C.S. Cheng, C.Y. Shih and F.L. Yeh, "Detection and partial characterization of a UV-damaged-DNA binding activity highly expressed in zebrafish (Danio rerio) embryos, Fish Physiol. Biochem., 25:41-51. (SCI)," , 2002.
31. Hsu, Todd* and Fu-Lung Yeh, "Differential regulation of spontaneous and heat-induced HSP 70 expression in developing zebrafish (Danio rerio), J. Exp. Zool., 293:349-359. (SCI)," , 2002.
32. Hsu YL and Hsu T, "PCR primers specific for the detection of Penaeus monodon-type baculovirus. U.S.A. Patent No. 6,284,455," , 2001.
33. Hsu, Y. L., K. H. Wang, Y. H. Yang, M. C. Tung, C. H. Hu, C. F. Lo, C. H. Wang, T. Hsu*, "Diagnosis of Penaeus monodon-type Baculovirus by PCR and by ELISA of Occlusion Bodies, Dis. Aquat. Org., 40: 93-99. (SCI)," , 2000.
34. Yeh, Fuh-Lung and Todd Hsu*, "Detection of a spontaneous high expression of heat shock protein 70 in developing zebrafish (Danio rerio), Biosci. Biotech. Biochem., 64: 592-595. (SCI)," , 2000.
35. Hsu, Todd*., Reou-Ching Sheu and Yi-Show Lai, "Possible involvement of a 72-kDa polypeptide in nucleotide excision repair of Chlorella pyrenoidosa identified by affinity adsorption and repair synthesis assay, Plant Sci., 156: 95-102. (SCI)," , 2000.
36. Ching-Feng Weng, Shao-Hsiang Liu, Todd Hsu and Ya-Li Hsu, "The existence of interleukin-2-like molecule in the Oka (lymphoid) organ of grass prawn (Penaeus monodon). COA Fisheries Series, R.O.C. 65:195-205.," , 1999.
37. Todd Hsu*, Jhon-Chun Ho and Chih-Chung Chao, "Purification of a UV-damaged-DNA binding activity from cell-free extracts of unicellular alga Chlorella pyrenoidosa. Plant Sci. 138:137-147. (SCI)," , 1998.
38. Todd Hsu*, Hua-Mei Huang and Chin-Hwa Hu, "Differential effects of heavy metals on the binding of Xenopus upstream binding factor (xUBF) to DNA. Chemosphere 36:2367-2373. (SCI)," , 1998.
Project
1. 熱休克蛋白70對魚類胚胎中DNA錯誤配對修補蛋白之分子監護效應 (2011-08 ~ 2012-07)
2. 小球藻G-T辨識蛋白多型性分析與受基因轉殖之影響 (2006-08 ~ 2007-07)
3. 大學工程生物教育整合型研究計畫--海洋生物微光機電特色學程研究(2/2) (2005-08 ~ 2006-07)
4. 以MSH2-MSH6競爭性吸附法分析小球藻DNA配對錯誤辨識蛋白群 (2005-08 ~ 2006-07)
5. 大學工程生物教育整合型研究計畫--海洋生物微光機電特色學程研究(1/2) (2004-08 ~ 2005-07)
6. 以MSH6競爭性吸附法分析小球藻DNA配對錯誤辨識蛋白 (2004-08 ~ 2005-07)
7. 小球藻6-4光合產物辨識蛋白分離與cDNA選殖 (2003-08 ~ 2004-07)
8. 低量紫外線C 早期引誘發小球藻蛋白特性鑑定 (2002-08 ~ 2003-07)
9. 以親和性吸附及交錯連結法研究單胞藻中DNA修補蛋白群之組成 (2001-08 ~ 2002-07)
10. 小球單胞藻DNA修補蛋白之親和性純化與功能鑑定 (2000-08 ~ 2001-07)
11. 單胞藻對DNA外接鍵結物之切割修補作用 (1999-08 ~ 2000-07)
12. 單胞藻DNA修補蛋白之純化及鑑定 (1998-08 ~ 1999-07)
13. 單胞藻修補紫外光損傷DNA作用之研究 (1996-08 ~ 1997-07)
14. 鎘破壞DNA作用機制之研究 (1991-08 ~ 1992-07)