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微生物與轉譯醫學實驗室 (Lab of Microbiology and Translational Medicine)

 

主持人: 許淳茹 老師

 

研究專長:

微生物學、細胞微生物學、分子生物學、微生物致病機轉、病原菌與宿主細胞交互作用

 

學歷:

英國劍橋大學病理學系微生物學與寄生蟲學組 博士

台灣大學醫學院微生物學研究所 碩士

台灣大學理學院植物學系 學士

 

經歷:

義守大學學士後醫學系外國學生專班 助理教授

義大醫院醫學研究部 助理研究員

台灣大學醫學院微生物所 博士後研究員

 

研究室簡介:

 

致病性微生物在臨床上往往造成許多嚴重的問題,抗藥性菌株的出現及抗藥基因的傳播也使現有抗生素的使用受到限制,增加感染症治療上的困難。本實驗室研究興趣主要包括微生物致病分子機轉、微生物與宿主細胞交互作用機制、開發抗藥性細菌之替代性治療物質或療法,以及環境、腸道正常菌叢與細菌致病之關連等,主要利用分子生物學、細胞學、免疫學、蛋白質表現純化、基因體學及動物實驗等系統性和整合性方法,探討微生物與疾病相關議題,希望將微生物學研究應用於健康醫療產業,發展疾病篩檢、診斷工具、治療藥物或疫苗,希望幫助解決健康醫療實務問題。我們的研究主題與方向:

 

1.      微生物致病機轉、致病因子、病原菌與宿主細胞交互作用 (microbial pathogenesis, virulence factors, pathogen-host interactions):

找出病原菌重要之致病因子,探討致病因子作用的分子機制及調控網路,以及探討病原菌如何影響宿主細胞之反應及訊息傳遞,藉此了解病原菌致病機轉,未來可應用於設計藥物或疫苗之標的(target)

 

2. 抗藥性細菌之替代性治療物質或方法 (alternative medicine; complementary medicine):

從中草藥與天然物中開發對抗藥性細菌有殺菌/抑菌潛力之物質,研究天然化合物之抗菌力與機制,針對抗藥性問題嚴重、可使用的藥物有限之臨床致病細菌,分析in vitro抗菌力、作用機制及in vivo動物試驗,進一步探討應用於感染症治療與控制之潛力。

 

3.      環境微生物、腸道正常菌叢菌株與致病菌之抗藥性分佈、致病力與多樣性:

隨著One Health (一體化健康、防疫一體)的概念越來越受到重視,認為人類、動物、環境的健康衛生關係是息息相關,這些面向的健康都需要監控與維護以達到疾病預防及緩減。我們希望探討環境、腸道正常菌叢(microflora)與造成感染之微生物之間的關連,藉由分析環境菌株、腸道菌株與感染菌株的特性與基因體,了解其致病力、抗藥性分佈、之間可能的傳播與演化,可應用於評估腸道微生物篩檢或環境檢測。

 

 

Publications (*correspondence)

 

Journal papers:

1.          Chun-Ru Hsu, I-Wei Chang, Pei-Fang Hsieh, Tzu-Lung Lin, Pei-Yin Liu, Chen-Hsiu Huang, Kun-Tzu Li1, Jin-Town Wang*. A novel role of the Klebsiella pneumoniae Sap transporter in intestinal cell interactions, innate immune responses, liver abscess, and virulence. Journal of Infectious Diseases, 2019; 219(8): 1294–1306. (IF=5.045, 11.2% in Infectious Diseases)

2.          Yi-Jiun Pan, Tzu-Lung Lin, Yi-Yin Chen, Peng-Hsuan Lai, Yun-Ting Tsai, Chun-Ru Hsu, Pei-Fang Hsieh, Yi-Tsung Lin, Jin-Town Wang*. Identification of three podoviruses infecting Klebsiella encoding capsule depolymerases that digest specific capsular types. Microbial biotechnology, 2019; 12(3): 472–486. (IF=4.857, 14% in Biotechnology & Applied Microbiology)

3.          Chin-Chuan Tsai, Chi-Shiuan Lin, Chun-Ru Hsu, Chiu-Ming Chang, I-Wei Chang, Li-Wei Lin, Chih-Hsin Hung*, Jiun-Ling Wang*. Using the Chinese herb Scutellaria barbata against extensively drug-resistant Acinetobacter baumannii infections: in vitro and in vivo studies. BMC Complementary and Alternative Medicine, 2018; 18: 96. (IF=2.479, 29.6% in Infectious Diseases)

4.          Chun-Ru Hsu, Chun-Hsing Liao, Tzu-Lung Lin, Han-Ru Yang, Feng-Ling Yang, Pei-Fang Hsieh, Shih-Hsiung Wu, Jin-Town Wang*. Identification of a capsular variant and characterization of capsular acetylation in Klebsiella pneumoniae PLA-associated type K57. Scientific Reports, 2016; 6: 31946. (IF=4.011, 21.7% in Multidisciplinary Sciences)

5.          Pei-Fang Hsieh, Chun-Ru Hsu, Chun-Tang Chen, Tzu-Lung Lin, Jin-Town Wang*. The Klebsiella pneumoniae YfgL (BamB) lipoprotein contributes to outer membrane protein biogenesis, type-1 fimbriae expression, anti-phagocytosis, and in vivo virulence. Virulence, 2016; 7(5): 587-601. (IF=4.775, 14.6% in Infectious Diseases)

6.          Tzu-Lung Lin, Yi-Jiun Pan, Pei-Fang Hsieh, Chun-Ru Hsu, Meng-Chuan Wu, Jin-Town Wang*. Imipenem represses CRISPR-Cas interference of DNA acquisition through H-NS stimulation in Klebsiella pneumoniae. Scientific Reports, 2016; 6: 31644. (IF=4.011, 21.7% in Multidisciplinary Sciences)

7.          Chun-Ru Hsu, Yi-Jiun Pan, Ju-Yun Liu, Jin-Town Wang*. Klebsiella pneumoniae translocates across the intestinal epithelium via Rho GTPase- and Phosphatidylinositol 3-kinase/Akt-dependent cell invasion. Infection and Immunity, 2015; 83(2): 769-779. (IF=3.16, 38.2% in Infectious Diseases)

8.          Yi-Jiun Pan, Tzu-Lung Lin, Chun-Tang Chen, Yi-Yin Chen, Pei-Fang Hsieh, Chun-Ru Hsu, Meng-Chuan Wu, Jin-Town Wang*. Genetic analysis of capsular polysaccharide synthesis gene clusters in 79 capsular types of Klebsiella spp. Scientific Reports, 2015; 5:15573. (IF=4.011, 21.7% in Multidisciplinary Sciences)

9.          Yi-Jiun Pan, Tzu-Lung Lin, Yi-Tsung Lin, Po-An Su, Chun-Tang Chen, Pei-Fang Hsieh, Chun-Ru Hsu, Ching-Ching Chen, Yu-Chia Hsieh, Jin-Town Wang*. Identification of capsular types in carbapenem-resistant Klebsiella pneumoniae strains by wzc sequencing and implications in capsule depolymerase treatment. Antimicrobial Agents and Chemotherapy, 2015; 59(2): 1038-1047. (IF=4.715, 10.1% in Pharmacology & Pharmacy)

10.      Tzu-Lung Lin, Pei-Fang Hsieh, Yu-Tsung Huang, Wei-Ching Lee, Yi-Ting Tsai, Po-An Su, Yi-Jiun Pan, Chun-Ru Hsu, Meng-Chuan Wu, Jin-Town Wang*. Isolation of a bacteriophage and its depolymerase specific for K1 capsule of Klebsiella pneumoniae: implication in typing and treatment. Journal of Infectious Diseases, 2014; 210(11): 1734-1744. (IF=5.045, 11.2% in Infectious Diseases)

11.      Chun-Ru Hsu, Tzu-Lung Lin, Yi-Jiun Pan, Pei-Fang Hsieh, Jin-Town Wang. Isolation of a Bacteriophage Specific for a New Capsular Type of Klebsiella pneumoniae and Characterization of Its Polysaccharide Depolymerase. PLoS One, 2013; 8(8): e70092. (IF=2.776, 34.8% in Multidisciplinary Sciences)

12.      Yi-Jiun Pan, Tzu-Lung Lin, Yan-Hua Chen, Chun-Ru Hsu, Pei-Fang Hsieh, Mon-Chuan Wu Jin-Town Wang*. Capsular Types of Klebsiella pneumoniae Revisited by wzc Sequencing. PLoS One, 2013; 8(12): e80670. (IF=2.776, 34.8% in Multidisciplinary Sciences)

13.      Chun-Ru Hsu, Tzu-Lung Lin, Yo-Chi Chen, Jin-Town Wang*. The role of Klebsiella pneumoniae rmpA in capsular polysaccharide synthesis and virulence revisited. Microbiology-SGM, 2011; 157: 3446-3457. (IF=1.922, 75.9% in Microbiology)

14.      Yi-Jiun Pan, Tzu-Lung Lin, Chun-Ru Hsu, Jin-Town Wang*. Use of a Dictyostelium model for isolation of genetic loci associated with phagocytosis and virulence in Klebsiella pneumoniae. Infection and Immunity, 2011; 79(3): 997-1006. (IF=3.16, 38.2% in Infectious Diseases)

15.      Chun-Ru Hsu, Tsong-Ming Lu, Lengsu William Chin, Chi-Chiang Yang*. Possible DNA Viral Factors of Human Breast Cancer. Cancers 2010, 2(2):498-512. (IF=6.162, 13.5% in Oncology)

16.      Yao-Hsu Yang, Chen-Fang Hung, Chun-Ru Hsu, Li-Chieh Wang, Ya-Hui Chuang, Yu-Tsan Lin, Bor-Luen Chiang*. A nationwide survey on epidemiological characteristics of childhood Henoch-Schönlein Purpura in Taiwan. Rheumatology (Oxford) 2005, 44(5):618-22. (IF=5.149, 12.9% in Rheumatology)

 

Conference posters:

1.          Dai-Yun Du, Ping-Hsuan Hsieh, Chen-Hsiu Huang, Ya-Ling Huang, Chun-Ru Hsu*. Potential inhibitory activity of natural compounds against multidrug-resistant Klebsiella pneumoniae. 24屆細菌學研討會; 2019, August (Kaohsiung).

2.          Rong-ju Tsai, Wan-Nan U. Chen, Chun-Ru Hsu*.烏梅對臨床抗藥性克雷白氏肺炎桿菌抗菌研究。24屆細菌學研討會; 2019, August (Kaohsiung).

3.          Chun-Ru Hsu*, Chen-Hsiu Huang, Kun-Tzu Li, Wei-Chen Liao, Pei-Yin Liu, Jin-Town Wang. The effects of Klebsiella pneumoniae kpn_02265 and c-di-GMP signalling on intestinal cell interactions and in vivo survival. 24屆細菌學研討會; 2019, August (Kaohsiung).

4.          Chun-Ru Hsu*, Jie-Yu Liu, Chen-Hsiu Huang, Kun-Tzu Li, Ya-Ling Huang, Hsiu-Fang Lin, Tsun-Mei Lin. Characterization of the potentials of Chinese medical herbs and natural compounds against drug-resistant rapidly growing mycobacteria. 探討中草藥與天然化合物抑制快速生長分枝桿菌抗藥性菌株之潛力。台灣微生物學會第五十週年慶會員大會暨學術研討會; 2018, November (Taipei).

5.          Chun-Ru Hsu, Pei-Fang Hsieh, Pei-Yin Liu, Tzu-Lung Lin, and Jin-Town Wang. Identification of Klebsiella pneumoniae genes responsible for interactions with the intestinal epithelial cells. EMBO/EMBL Symposium 2017: New Approaches and Concepts in Microbiology; 2017, June (Germany).

6.          Chun-Ru Hsu, Tzu-Lung Lin, Yo-Chi Chen, and Jin-Town Wang. The role of Klebsiella pneumoniae RmpA/A2 in virulence and capsular types: different associations between the Asian and Western strains. European Conference of Microbiology and Immunology; 2011, May (Hungary).

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近三年研究計畫

 

計畫名稱

補助單位

執行期間

微生物致病機轉

第二訊息分子合成蛋白「雙鳥苷酸環化酶」多基因於克雷白氏肺炎桿菌致病力扮演之角色

科技部

2020/08/01-2021/07/31

探討克雷伯氏肺炎桿菌運輸蛋白SapTrk對宿主細胞交互作用及致病過程之影響與作用機制

科技部

2018/08/01-2020/07/31

克雷伯氏肺炎桿菌與宿主細胞之交互作用: 探討克雷伯氏肺炎桿菌存活於腸道上皮細胞之分子機轉

科技部

2016/11/01-2018/07/31

克雷柏氏菌肺炎桿菌第六型分泌系統對於致病力所扮演之角色

台大義大教研合作計畫

2018/07/01-2019/06/30

抗菌物質

天然化合物應用於克雷白氏肺炎桿菌臨床抗藥性菌株治療之潛力:評估抑菌效能及與抗生素之協同作用

義大醫院 (共同主持人)

2019/03/01-2020/2/29

探討芳香醛抑制分枝桿菌之活性與作用機轉

義大醫學生獎學金計畫

2018/07/01-2019/06/30

牛樟萃取物之抗菌效果分析

義守大學寬崎科技產學合作計畫

2018/08/01-2019/07/31

腸道菌與環境微生物

探討南台灣環境克雷伯氏肺炎桿菌抗藥性與基因體多樣性

成大義大教研合作計畫

2020/01/01-2020/12/31

義守大學外國學生之腸道克雷白氏肺炎桿菌多樣性調查

義大醫學生獎學金計畫

2019/07/01-2020/06/30

探討人類腸道正常菌叢之克雷伯氏肺炎桿攜帶率、致病性、抗藥性與基因體多樣性

義大醫院 (共同主持人)

2020/01/01-2020/12/31