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1. 研究介紹

環境毒物所引起生物體組織形態異常通常由分子層次如DNA傷害累積而成,尤其是胚胎期生物DNA分裂活動旺盛且對毒性傷害特別敏感,本實驗室以斑馬魚(Danio rerio)為模式生物,探討發育中脊椎動物之DNA錯誤配對與核酸切割修補系統之作用機制。研究重點為斑馬魚DNA錯誤配對辨識蛋白MSH2MSH6分子選殖,以全覆式原位雜交法探討配對錯誤修補蛋白基因於緊迫及非緊迫狀態下在胚胎之組織特異性表現與利用顯微注射反意核甘酸探討抑制胚胎中MSH2MSH6活性對神經系統基因表現之影響。另外本實驗室也藉親合性吸附法配合二維蛋白電泳與胜質譜對斑馬魚胚胎中可能參與核酸切割修補之蛋白進行分離及鑑定。

 
2. 期刊論文

Todd Hsu*, Hua-Mei Huang and
Chin-Hwa Hu (1998) Differential effects of heavy metals on the binding of
Xenopus upstream binding factor (xUBF) to DNA. Chemosphere 36:2367-2373. (SCI)
 

Todd Hsu*, Jhon-Chun Ho and
Chih-Chung Chao (1998) Purification of a UV-damaged-DNA binding activity from
cell-free extracts of unicellular alga Chlorella pyrenoidosa. Plant Sci.
138:137-147. (SCI)
 

Ching-Feng Weng, Shao-Hsiang Liu,
Todd Hsu and Ya-Li Hsu (1999) The existence of interleukin-2-like molecule in
the Oka (lymphoid) organ of grass prawn (Penaeus monodon). COA Fisheries
Series, R.O.C. 65:195-205.
 

Hsu, Todd*., Reou-Ching Sheu and
Yi-Show Lai (2000) Possible involvement of a 72-kDa polypeptide in nucleotide
excision repair of Chlorella pyrenoidosa identified by affinity adsorption and
repair synthesis assay, Plant Sci., 156: 95-102. (SCI)
 

Yeh, Fuh-Lung and Todd Hsu*
(2000) Detection of a spontaneous high expression of heat shock protein 70 in
developing zebrafish (Danio rerio), Biosci. Biotech. Biochem., 64: 592-595.
(SCI)
 

Hsu, Y. L., K. H. Wang, Y. H.
Yang, M. C. Tung, C. H. Hu, C. F. Lo, C. H. Wang, T. Hsu* (2000) Diagnosis of
Penaeus monodon-type Baculovirus by PCR and by ELISA of Occlusion Bodies, Dis.
Aquat. Org., 40: 93-99. (SCI)
 

Hsu YL and Hsu T (2001) PCR
primers specific for the detection of Penaeus monodon-type baculovirus. U.S.A.
Patent No. 6,284,455
 

Hsu, Todd* and Fu-Lung Yeh (2002)
Differential regulation of spontaneous and heat-induced HSP 70 expression in
developing zebrafish (Danio rerio), J. Exp. Zool., 293:349-359. (SCI)
 

Hsu, T*., C.S. Cheng, C.Y. Shih
and F.L. Yeh (2002) Detection and partial characterization of a UV-damaged-DNA
binding activity highly expressed in zebrafish (Danio rerio) embryos, Fish
Physiol. Biochem., 25:41-51. (SCI)
 

Yeh, Fu-Lung., Hwei-Ling Yan,
Shi-Ya Wang, Ting-Yi Jung and Todd Hsu* (2003) Molecular cloning of
zebrafish(Danio rerio) MutS homolog 6(MSH6) and noncoordinate expression of
MSH6 gene activity and G-T mismatch binding proteins in zebrafish larvae, J.
Exp. Zool., 297A: 118-129. (SCI)
 

Yeh,
Fu-Lung., Shi-Ya Wang, Lung-Ying Hsu, Dar-Yi Wang and Todd Hsu* (2004) Cloning
of the mismatch recognition protein MSH2 from zebrafish(Danio rerio) and its
developmental stage-dependent mRNA expression, Biochim. Biophys. Acta (Gene
Structure and Expression)., 1680: 129-136.(SCI)
 

Chen,
F.P., T. Hsu, C.H. Hu, W.D. Wang, K.C. Wang and L.F. Teng (2004) Expression of
estrogen receptors alpha and beta in human osteoblasts: identification of
exon-2 deletion variant of estrogen receptor beta in postmenopausal women,
Chang Gung Med. J., 27: 107-115.
 

Chiou L.M, M.C. Chung, P.G. Tung,
T. Hsu and J.S. Yang (2004) The use of egg chorion glycoprotein of Epinephelus
malabaricus for egg identification, J. Fish Biol., 65:1614-1621. (SCI)
 

Hsu, Todd*., Kai-Ning Chang,
Yi-Show Lai, Ting-Yi Jung and Gen-I Lee (2005) DNA mismatch binding activities
in Chlorella pyrenoidosa extracts and affinity isolation of G-T mismatch
binding proteins, Plant Physiol. Biochem., 43:309-313 (SCI)

 

Wu Chien-Wei, Li-Jung Yin, Todd
Hsu, Shann-Tzong Jiang (2005) Comparative Study on Antibacterial Effect of
Pediocin ACCEL and Nisin Against Fluorescencestained Listeria monocytogenes
BCRC 14845. J. Food Sci., 70: 75-80 (SCI)
 

Chen, Fang-Ping., Todd Hsu,
Chin-Hwa Hu, Wen-Der Wang, Kun-Chuang Wang, Li-Fen Teng (2006) Expression of
estrogen receptors alfa and beta mRNA and alkaline phosphatase in the
differentiation of osteoblasts from elderly postmenopausal women: comparison
with osteoblasts from osteosarcoma cell line. Taiwanese J. Obstet. Gynecol.
45:307-312.
 

Lai, Yi-Show., Li-Feng Chiue and
Todd Hsu* (2006)Low-molecular-weight vitellogenin 1-like proteins are
components of a UV-damaged-DNA binding activity highly expressed in zebrafish
(Danio rerio) embryos, J. Exp. Zool., 306A: 215-224. (SCI)
 

Yeh, Fu-Lung., Lung-Yin Hsu,
Bo-An Lin, Chiao-Fu Chen, I-Chen Li, Shin-Hua Tsai and Todd Hsu* (2006) Cloning
of zebrafish (Danio rerio) heat shock factor 2 (HSF2) and similar patterns of
HSF2 and HSF1 mRNA expression in brain tissues. Biochimie 88:1983-1988.(SCI)
 

Chang, J. W-C., C-L. Chou, S-F.
Huang, H-M. Wang, J-J. Hsieh, T. Hsu, Y-C. Cheung (2007) Erlotinib response of
EGFR-mutant gefitinib-resistant non-small-cell lung cancer, Lung Cancer
58:414-417. (SCI)
 

Liu, A-C., H-W Shih, T. Hsu, E-M
Lai (2008) A citrate-inducible gene, encoding a putative tricarboxylate
transporter, is downregulated by the organic solvent DMSO in Agrobacterium
tumefaciens, J. Appl. Microbiol. 105: 1372-1383. (SCI)
 

Chen, C-M., J.W-C. Chang, Y-C.
Cheung, G. Lin, J-J. Hsieh, T. Hsu, S-F. Huang (2008) Computed
tomography-guided core-needle biopsy specimens demonstrate epidermal growth
factor receptor mutations in patients with non-small-cell lung cancer, Acta
Radiologica 49:991-994. (SCI)
 

Chang, Wen-Cheng., Jia-Juan
Hsieh, Yung-Chi Shen, Kun-Yun Yeh, Cheng-Hsu Wang, Ying-Ying Li, Todd Hsu*
(2009) Bisphosphonate zoledronic acid enhances the inhibitory effects of
gefitinib on EGFR-mutated non-small cell lung carcinoma cells, Cancer Lett.
278:17-26. (SCI)
 

Katarzyna Kazłowska, Todd Hsu,
Chia-Chung Hou, Wen-Chin Yang, Guo-Jane Tsai (2010) Anti-inflammatory
properties of phenolic compounds and crude extract from Porphyra dentate, J.
Ethnopharmacol. 128: 123-130. (SCI)
 

Hsu, Lung-Yin., Fu-Lung Yeh,
Tun-Yuan Cheng, You-Hsin Chang, Shiu-Mei Liu, Todd Hsu* (2010) A spontaneous
high expression of heat shock cognate 70 (HSC 70) in zebrafish Danio rerio
larvae arising from tissue-specific translation of preexisting mRNA, Fisheries Sci.
76: 857-864.(SCI)
 

Hou, Ming-Mo., Jia-Juan Hsieh,
Nai-Jen Chang, Hsuan-Ying Huang, Hung-Ming Wang, Cheng-Keng Chang, Todd Hsu,
John Wen-Cheng Chang (2010) Response to sorafenib in a patient with metastatic
Xp11 translocation renal cell carcinoma, Clin. Drug Investig. 30:799-804.(SCI)
 

Todd Hsu*, Huei-Ting Tsai,
Kuan-Ming Huang, Mei-Chu Luan, Chang-Ruei Hsieh (2010) Sublethal levels of
cadmium down-regulate the gene expression of DNA mismatch recognition protein
MutS homolog 6 (MSH6) in zebrafish (Danio rerio) embryos, Chemosphere 81: 748-754.(SCI)
 

Hsieh, Chia-Hsun, John W-C Chang,
Jia-Juan Hsieh, Todd Hsu, Shiu-Feng Huang, Chun-Ta Liao, Hung-Ming Wang (2011)
Epidermal growth factor receptor mutations in patients with oral cavity cancer
in a bet nut chewing-prevalent area, Head and Neck 33:1758-1764.(SCI)
 

Yu, Chun-Kang, Kan-Hung Hu,
Shing-Hoa Wang*, Todd Hsu*, Huei-Ting Tsai, Chien-Chou Chen, Shiu-Mei Liu,
Tai-Yuan Lin, Chin-Hsing Chen (2011) Photocatalytic effect of anodic titanium
oxide nanotubes on various cell culture media, Appl. Phys. (A) 102:
271-274.(SCI)
 

Chang, Wen-Cheng, Nien-Chih Wei,
Hung-Ju Su, Jie-Len Huang, Tse-Ching Chen, Yi-Cheng Wu, Ming-Mo Hou, Chia-Hsun
Hsien, Jia-Juan Hsieh, Chiao-En Wu, Todd Hsu, Tzu-Hao Wang (2012)Comparison of
genomic signatures of non-small cell lung cancer recurrence between two
microarray platforms. Anticancer Res. 32: 1259-1266.(SCI)
 

Lai, Yi-Show, Feng-Ju Hsieh, Todd
Hsu* (2012) Affinity isolation and mass spectral analysis of
1,10-phenanthroline (OP)-stimulated UV-damaged-DNA binding proteins expressed
in zebrafish (Danio rerio) embryos. Fish Physiol. Biochem. 38: 1117-1129. (SCI)
NSC 95-2313-B-019-003, NSC 100-2313-B-019-009
 

Hou, Ming-Mo, Shiu-Feng Huang,
Han-Pin Kuo, Cheng-Ta Yang, Ying-Huang Tsai, Chih-Teng Yu, Horng-Chyuan Lin,
Chih-Hung Chen, Chih-Liang Wang, Fu-Tsai Chung, Jia-Juan Hsieh, Todd Hsu,
Hsin-Yi Cheng, Li-Ying Ou, Hung-ming Wang, Yung-Chang Lin, Nai-Jen Chang, John
Wen-Cheng Chang (2012)Erlotinib treatment in patients with advanced lung
adenocarcinoma with CISH-positive and CISH-negative EGFR gene alterations.
Anticancer Res. 32: 1107-1112.(SCI)
 

Kan-Hung Hu, Shing-Hoa Wang, Todd
Hsu, Chien-Chon Chen, Tai-Yuan Lin, Huei-Ting Tsai, Hsiang-Ho Chiang (2012) The
effect of Nitrogen-Doped ATO Nanotubes on Radical Multiplication of Buffer
Media by Visible Light Photocatalysis Rather UV. J. Nanomater. 2012, Article ID
510672, 6 pages.(SCI)

Todd Hsu,
Kuan-Ming Huang, Huei-Ting Tsai, Shih-Tsung Sung, Tsung-Nan Ho (2013)
Cadmium(Cd)-induced oxidative stress down-regulates the gene expression of DNA
mismatch recognition proteins MutS homolog 2 (MSH2) and MSH6 in zebrafish
(Danio rerio) embryos. Aquat. Toxicol. 126:9-16. (SCI)

Wu,Chiao-En, Chi-Wei Wang, Wen-Kuan Huang,
Cheng-Ta Yang, Yi-Cheng Wu, Ming-Mo Hou, Chia-Hsun Hsieh, Jia-Juan Hsieh,
Hsin-Yi Cheng, Todd Hsu, John Wen-Cheng Chang (2015) Cytoplasmic and nuclear
parathyroid hormone-related proteins are opposing prognostic factors in
patients with non-small-cell lung cancer who have undergone curative resection.
Jpn. J. Clin. Oncol. 45(3): 267-273. (SCI)

Tsung-Nan Ho, Shin-Tsung Sung, Kuan-Ming Huan, Todd Hsu* (2015) Comparative effects of mercury (ll) and cadmium on Muts homolog 6(Msh6)-mediate DNA mismatch binding activities in zebrafish (Danio rerio) embryo. J. Biochem. Mol. Toxicol. 29:513-520(SCI)

Li-Bin Ling, Yung Chang, Chia-Wei Liu, Po-Ling Lai, Todd Hsu*(2017)Oxidative stress intensity-related effects of cadmium (Cd) andparaquat (PQ) on UV-damaged-DNA binding and excision repair activities in zebrafish (Danio rerio) embryos. Chemosphere 167 (2017) 10-18

Yung-Chi Shen, Todd Hsu*, Lin-Bin Ling, Wen-Chian You, Chia-Wei Liu (2017) Identification of Low-molecular-weight vitellogenin 1(Vg1)-like proteins as nucleotide excision repair factors in developing zebrafish (Danio rerio) using a transcription-based DNA repair assay. Fish Physiolgy and Biochemistry.

Yung Chang, Wei-Yuan Lee, Yu-Jie Lin, Todd Hsu* (2017) Mercury (II) impairs nucleotide excision repair (NER) in zebrafish (Danio rerio) embryos by targeting primarily at the stage of DNA incision. Aquatic Toxicology.

   
3. 研究計畫

80年度專題研究計畫(一般型研究計畫) 計畫名稱:鎘破壞DNA作用機制之研究



85年度專題研究計畫(一般型研究計畫) 計畫名稱:單胞藻修補紫外光損傷DNA作用之研究



87年度專題研究計畫(新進人員研究計畫) 計畫名稱:單胞藻DNA修補蛋白之純化及鑑定



88年度專題研究計畫(新進人員研究計畫) 計畫名稱:單胞藻對DNA外接鍵結物之切割修補作用



89年度專題研究計畫(一般型研究計畫) 計畫名稱:小球單胞藻DNA修補蛋白之親和性純化與功能鑑定



90年度專題研究計畫(一般型研究計畫) 計畫名稱:以親和性吸附及交錯連結法研究單胞藻中DNA修補蛋白群之組成



91年度專題研究計畫(一般型研究計畫) 計畫名稱:低量紫外線C 早期引誘發小球藻蛋白特性鑑定



92年度專題研究計畫(一般型研究計畫 ) 計畫名稱:小球藻6-4光合產物辨識蛋白分離與cDNA選殖



93年度專題研究計畫(一般型研究計畫) 計畫名稱:MSH6競爭性吸附法分析小球藻DNA配對錯誤辨識蛋白



93年度專題研究計畫(政策導向型科學教育計畫)-共同主持人 
計畫名稱:大學工程生物教育整合型研究計畫--海洋生物微光機電特色學程研究(1/2)



94年度專題研究計畫(一般型研究計畫) 計畫名稱:MSH2-MSH6競爭性吸附法分析小球藻DNA配對錯誤辨識蛋白群



94年度專題研究計畫(政策導向型科學教育計畫)-共同主持人 計畫名稱:大學工程生物教育整合型研究計畫--海洋生物微光機電特色學程研究(2/2)



95年度專題研究計畫(一般型研究計畫) 計畫名稱:小球藻G-T辨識蛋白多型性分析與受基因轉殖之影響



100年度專題研究計畫(一般型研究計畫) 計畫名稱:熱休克蛋白70對魚類胚胎中DNA錯誤配對修補蛋白之分子監護效應